Erik A. Johannessen

Toward a miniature wireless integrated multisensor microsystem for industrial and biomedical applications

This paper presents our work toward the integration of a multisensor microsystem with wireless communication, using system-on-chip (SoC) methodology. Four different forms of microelectronic sensors have been fabricated on two separate 5/spl times/5 mm/sup 2/ silicon chips measuring pH, conductivity, dissolved oxygen concentration, and temperature. The sensors are integrated with a sensor fusion chip comprising analog circuitry for sensor operation and signal amplification prior to digital decoding and transmission. The microsystem prototype will be packaged in a miniature capsule, which measures 16 mm /spl times/55 mm including batteries and dissipates 6.3 mW for a minimal life cycle of 12 h.

Heat conduction nanocalorimeter for pl-scale single cell measurements

An ultrasensitive nanocalorimeter for use with pl-scale biological samples using silicon microfabrication technology has been developed in which a 720 pl reaction vessel, a calibration heater, and a thermoelectric transducer of 125 μK sensitivity were integrated into a single multilayer thin-film configuration. The resolution of the system ranged from 10 to 25 nW depending on the heat capacity, conductance and power density of the samples studied. The device has been used in heat conduction measurements of the energy released from the enzyme catalyzed hydrolysis of hydrogen peroxide using purified catalase, and for the determination of the catalase activity within a single mouse hepatocyte. The nanocalorimeter has the potential for integration in a high-density array format, where the change in temperature from ultralow volume cellular assays could be used as a generic analytical tool for high throughput screening of bioactive compounds.

Integrated micro-instrumentation for dynamic monitoring of the gastro-intestinal tract

The introduction of microsystem technology into diagnostic devices is a rapidly growing field where low form-factor can significantly improve device access or patient comfort. In this paper we present our results on a lab-in-a-pill device that uses laboratory-on-a-chip and system-on-chip technology to deliver analytical data from a range of sensors, and the methodology employed to build the device.

Biocompatibility of a Lab-on-a-Pill Sensor in Artificial Gastrointestinal Environments

In this paper, we present a radiotelemetry sensor, designed as a lab-in-a-pill, which incorporates a two-channel microfabricated sensor platform for real-time measurements of temperature and pH. These two parameters have potential application for use in remote biological sensing (for example they may be used as markers that reflect the physiological environment or as indicators for disease, within the gastrointestinal tract). We have investigated the effects of biofouling on these sensors, by exploring their response time and sensitivity in a model in vitro gastrointestinal system. The artificial gastric and intestinal solutions used represent a model both for fasting, as well as for the ingestion of food and subsequent digestion to gastrointestinal chyme. The results showed a decrease in pH sensitivity after exposure of the sensors for 3 h. The response time also increased from an initial measurement time of 10 s in pure GI juice, to ca. 25 s following the ingestion of food and 80 s in simulated chyme. These in vitro results indicate that changes in viscosity in our model gastrointestinal system had a pronounced effect on the unmodified sensor

An integrated sensor microsystem for industrial and biomedical applications

There is considerable interest in the development of ultra-miniature and low-power sensor microsystems for use in applications such as medical diagnostics, environmental monitoring and other industrial applications. Such ultra-miniature sensor microsystems must contain a large diversity of complex electronics, including sensor interfaces, signal conditioning, a microprocessor core, digital signal processing, and wireless transmission technology. In this paper, we will describe the first steps towards the development of a System on Chip for such a sensor microsystem and the methodology employed to build such a microsystems.

Implementation of radiotelemetry in a lab-in-a-pill format.

A miniaturised lab-in-a-pill device has been produced incorporating a temperature and pH sensor with wireless communication using the 433.92 MHz ISM band. The device has been designed in order to enable real time in situ measurements in the gastrointestinal (GI) tract, and accordingly, issues concerning the resolution and accuracy of the data, and the lifetime of the device have been considered. The sensors, which will measure two key parameters reflecting the physiological environment in the GI (as indicators for disease) were both controlled by an application specific integrated circuit (ASIC). The data were sampled at 10-bit resolution prior to communication off chip as a single interleaved data stream. This incorporated a power saving serial bitstream data compression algorithm that was found to extend the service lifetime of the pill by 70%. An integrated on-off keying (OOK) radio transmitter was used to send the signal to a local receiver (base station), prior to acquisition on a computer. A permanent magnet was also incorporated in the device to enable non-visual tracking of the system. We report on the implementation of this device, together with an initial study sampling from within the porcine GI tract, showing that measurements from the lab-on-a-pill, in situ, was within 90% of literature values.

An Ingestible Electronic Pill for Real Time Analytical Measurements of the Gastro-Intestinal Tract

A state-of-the-art electronic “pill” has been developed for in situ studies of the gastrointestinal (GI) tract using integrated circuit and system level integration technologies. The measurement parameters include real time analysis of temperature, pH, conductivity and dissolved oxygen.

A sensor system on chip for wireless microsystems

Recent years have seen the rapid development of microsensor technology, system on chip design, wireless technology and ubiquitous computing. When assembled into a complex microsystem the technologies become powerful tools in medical diagnostics, environmental monitoring and personal connectivity. In this paper we describe the demonstration of a silicon chip that has all the attributes required of a microsystem for use in these applications. The design methodology we have employed is a variant of the system on chip approach whereby many intellectual property blocks are integrated at a high level in the design flow. Our intellectual property blocks include the analogue sensor instrumentation for temperature and pH, a data multiplexing and conversion module, a digital platform based around an 8-bit microcontroller, data encoding for spread-spectrum wireless transmission and a RF section requiring very few off-chip components. The chip has been fully evaluated and tested by connection to external sensors. Each block has well defined interfaces so that they can be easily reused in future designs targeted to different applications

Bridging the Gap Between Micro and Nanotechnology: Using Lab-on-a-Chip to Enable Nanosensors for Genomics, Proteomics, and Diagnostic Screening

The growing need for accurate and fast methods of DNA and protein determination in the post human genome era has generated considerable interest in the development of new microfluidic analytical platforms, fabricated using methods adapted from the semi-conductor industry. These methods have resulted in the development of the Lab-on-a-Chip concept, a technology which often involves having a miniaturised biochip (as an analytical device), with rather larger instrumentation associated with the control of the associated sensors and of fluidics. This talk will explore the development of new Lab-on-a-Chip platforms for DNA, protein and cell screening, using microfluidics as a packaging technology in order to enable advances in nanoscale science to be implemented in a Lab-on-a-Chip format. The talk will also show how system on a chip methods can be integrated with Lab-on-a-Chip devices to create remote and distributed intelligent sensors, which can be used in a variety of diagnostic applications, including for example chemical sensing within the GI tract.

Ultra-Low Volume Nanocalorimetric Measurements for Screening Cellular Metabolic Activty

A microsystems sensor technology for cellular screening of bioactive compounds is presented which combines extreme sensitivity with low volume, fast response time and improved ease of manufacturing.