Erik Dahl Kjær

Towards domestication of Jatropha curcas

Jatropha curcas L. attracts a lot of interest as a biofuel crop, triggering large investments and rapid expansion of cultivation areas, and yet, it should still be considered as a (semi-)wild, undomesticated plant. To use the full potential of Jatropha and to support further expansion and systematic selection, breeding and domestication are a prerequisite. This review reveals and identifies gaps in knowledge that still impede domestication of Jatropha. Prebreeding knowledge is limited. In particular, the regeneration ecology and the degree of genetic diversity among and within natural populations in and outside the center of origin are poorly studied. There is only a limited understanding of the Jatropha breeding system and the effect of inbreeding and outbreeding. This review presents all currently available and relevant information on the species distribution, site requirements, regeneration ecology, genetic diversity, advances in selection, development of varieties and hybridization. It also describes possible routes to a better Jatropha germplasm, gives recommendations for tackling current problems and provides guidance for future research. We also discuss the participatory domestication strategy of Jatropha integration in agroforestry.

Adaptive potential of ash (Fraxinus excelsior) populations against the novel emerging pathogen Hymenoscyphus pseudoalbidus.

An emerging infectious pathogen Hymenoscyphus pseudoalbidus has spread across much of Europe within recent years causing devastating damage on European common ash trees (Fraxinus excelsior) and associated plant communities. The present study demonstrates the presence of additive genetic variation in susceptibility of natural F. excelsior populations to the new invasive disease. We observe high levels of additive variation in the degree of susceptibility with relatively low influence of environmental factors (narrow‐sense heritability = 0.37–0.52). Most native trees are found to be highly susceptible, and we estimate that only around 1% has the potential of producing offspring with expected crown damage of <10% under the present disease pressure. The results suggest that the presence of additive genetic diversity in natural F. excelsior populations can confer the species with important ability to recover, but that low resistance within natural European populations is to be expected because of a low frequency of the hypo‐sensitive trees. Large effective population sizes will be required to avoid genetic bottlenecks. The role of artificial selection and breeding for protection of the species is discussed based on the findings.

Hybridization and genetic variation in Danish populations of European crab apple (Malus sylvestris)

The aim of the present study was to investigate the genetic variation in Danish populations of the endangered European crab apple (Malus sylvestris). Special emphasis was given to hybridization between the wild species and its cultivated relative Malus ×domestica. A total of 178 wild individuals from four Danish populations were studied along with a reference sample of 29 old cultivars. The genetic variation within and among samples was studied at ten microsatellite marker loci. Additionally, a morphological analysis was carried out to identify hybrids and test for correspondence between phenotypic and genotypic indices of hybridization. From application of ordination and a model-based cluster analysis to the molecular data, two clusters were identified consisting of wild and cultivated individuals, respectively. This indicates that pronounced admixture between the two species is not present. At the population level, a high correspondence was found between geographic isolation from M. ×domestica and genotypic and morphological indices of hybridization. As expected, isolated populations appeared less affected by hybridization than poorly isolated populations. Isolated “pure” M. sylvestris populations could thus be identified. However, morphological and molecular evidences of hybridization were found to be divergent at the individual level. This is suggestive of some historical introgression into the M. sylvestris gene pool and indicates that relying exclusively on either morphological or molecular characters as diagnostic markers in studies of hybridization between M. ×domestica and M. sylvestris might lead to fallible results. Combined application of genetic and morphological markers is therefore recommended.

Estimation of effective population number in a Picea abies (Karst.) seed orchard based on flower assessment

Equations for estimation of effective population numbers from relative gamete contribution are discussed. Numbers of male and female strobili were observed in years with good, medium and poor flowering in a clonal seed orchard of Picea abies (Karst.). Genetic variation in flowering intensity was measured, and the inbreeding and variance effective population number (N e (i) and N e (v)) were calculated based on the relative flowering. The inbreeding effective population number was approximately one half of the actual number of clones (the census number) in both the year with poor and the year with abundant flowering, but three‐quarters of the census number in a year with intermediate flowering. The variance effective population number fluctuated according to the abundance of flowers. N e (v) was 37% of the census number in the year with poor flowering, but 130% in the year with abundant flowering, and close to the census number (90%) in the year with intermediate flowering.

A greener Greenland? Climatic potential and long-term constraints on future expansions of trees and shrubs.

Warming-induced expansion of trees and shrubs into tundra vegetation will strongly impact Arctic ecosystems. Today, a small subset of the boreal woody flora found during certain Plio-Pleistocene warm periods inhabits Greenland. Whether the twenty-first century warming will induce a re-colonization of a rich woody flora depends on the roles of climate and migration limitations in shaping species ranges. Using potential treeline and climatic niche modelling, we project shifts in areas climatically suitable for tree growth and 56 Greenlandic, North American and European tree and shrub species from the Last Glacial Maximum through the present and into the future. In combination with observed tree plantings, our modelling highlights that a majority of the non-native species find climatically suitable conditions in certain parts of Greenland today, even in areas harbouring no native trees. Analyses of analogous climates indicate that these conditions are widespread outside Greenland, thus increasing the likelihood of woody invasions. Nonetheless, we find a substantial migration lag for Greenlands current and future woody flora. In conclusion, the projected climatic scope for future expansions is strongly limited by dispersal, soil development and other disequilibrium dynamics, with plantings and unintentional seed dispersal by humans having potentially large impacts on spread rates.

Advances in domestication of indigenous fruit trees in the West African Sahel

Fruit trees play an important nutritional role for livelihoods of rural people in the West African Sahel through provision of energy and nutrients such as vitamins, minerals, and proteins. Research on the domestication of local fruit trees has started recently through projects concentrating on some of the most important indigenous species of dry West Africa, i.e. Adansonia digitata, Parkia biglobosa, Tamarindus indica, Vitellaria paradoxa and Ziziphus mauritiana. We present a status of finalised and ongoing domestication research with the aim of defining research gaps that would need to be covered by future research activities to obtain higher yields and better quality fruits. Germplasm collection in central West Africa has been intense compared to elsewhere in the species’ distribution areas, but conservation status of the material is poor since it is only planted in few trials. Knowledge of genetic parameters, especially for fruit traits, is almost absent, but characterisation of genotypes is underway for some of the species. Mating systems and patterns are still unknown for many species. Efficient vegetative propagation based on simple techniques was shown to be possible for all species except P. biglobosa. In order to secure immediate as well as long term gains, we recommend combining clonal propagation of selected plus individuals with recombination and breeding of selected genotypes. We discuss whether local institutions in the Sahel have the financial capacity to carry out long term breeding programmes, and suggest that efforts should be made to find new ways of disseminating improved germplasm.

Genome sequence and genetic diversity of European ash trees

Ash trees (genus Fraxinus, family Oleaceae) are widespread throughout the Northern Hemisphere, but are being devastated in Europe by the fungus Hymenoscyphus fraxineus, causing ash dieback, and in North America by the herbivorous beetle Agrilus planipennis. Here we sequence the genome of a low-heterozygosity Fraxinus excelsior tree from Gloucestershire, UK, annotating 38,852 protein-coding genes of which 25% appear ash specific when compared with the genomes of ten other plant species. Analyses of paralogous genes suggest a whole-genome duplication shared with olive (Olea europaea, Oleaceae). We also re-sequence 37 F. excelsior trees from Europe, finding evidence for apparent long-term decline in effective population size. Using our reference sequence, we re-analyse association transcriptomic data, yielding improved markers for reduced susceptibility to ash dieback. Surveys of these markers in British populations suggest that reduced susceptibility to ash dieback may be more widespread in Great Britain than in Denmark. We also present evidence that susceptibility of trees to H. fraxineus is associated with their iridoid glycoside levels. This rapid, integrated, multidisciplinary research response to an emerging health threat in a non-model organism opens the way for mitigation of the epidemic.

Innovation in input supply systems in smallholder agroforestry: seed sources, supply chains and support systems

Institutional innovation in providing inputs and services is a central element for smallholder development. Agroforestry is an important income generating activity for millions of smallholders in the tropics, yet access to quality planting material—germplasm—of valuable tree species remains a major hurdle for improving farm productivity. We discuss requirements and possibilities for institutional innovation in developing more efficient delivery systems for tree germplasm as one aspect of improved input supply. We describe a simple model for delivery to farmers that identifies the major types of germplasm sources and discuss how this model can be used to identify relevant interventions to address bottlenecks in current systems. Our analysis leads to eight input supply configurations for smallholder agroforestry, typified by three major models. Lessons from the evolution of smallholder crop seed delivery systems can be applied to tree germplasm supply and indicate that a commercial, decentralised model holds most promise for sustainability. However, current emphasis in agroforestry on government and NGO models of delivery hinder the development of this approach. The application of prevailing classification approaches may also create a barrier to the development of appropriate supply systems that effectively service smallholders. An important implication of our analysis is that current actors in agroforestry input supply systems must redefine their roles in order for effective delivery to take place. We chose a case study from Kenya to illustrate our points.

Molecular markers for tolerance of European ash (Fraxinus excelsior) to dieback disease identified using Associative Transcriptomics.

Tree disease epidemics are a global problem, impacting food security, biodiversity and national economies. The potential for conservation and breeding in trees is hampered by complex genomes and long lifecycles, with most species lacking genomic resources. The European Ash tree Fraxinus excelsior is being devastated by the fungal pathogen Hymenoscyphus fraxineus, which causes ash dieback disease. Taking this system as an example and utilizing Associative Transcriptomics for the first time in a plant pathology study, we discovered gene sequence and gene expression variants across a genetic diversity panel scored for disease symptoms and identified markers strongly associated with canopy damage in infected trees. Using these markers we predicted phenotypes in a test panel of unrelated trees, successfully identifying individuals with a low level of susceptibility to the disease. Co-expression analysis suggested that pre-priming of defence responses may underlie reduced susceptibility to ash dieback.

A systematic approach to the conservation of genetic resources of trees and shrubs in Denmark

Abstract A Strategy for the Conservation of Genetic Resources of Trees and Shrubs in Denmark was prepared in 1991/93. The objective of the strategy is to secure the ability of the species covered to adapt to environmental changes, and to maintain the basis for future improvement work. The strategy includes 75 tree and shrub species of actual or potential use for planting in Denmark. Unless protective measures are taken, the size and constitution of the genetic variation of these species will, as a result of present human influence, most likely be affected in the medium-to-long term in a way that may reduce their adaptability and utility significantly. The article focuses on the systematic approach to gene resource conservation taken in Denmark. This approach provides an overview of the conservation effort required, estimated as a number of conservation stands with a minimum size and a specified geographical distribution for each species. A review of human influence on the forest genetic resources since early forest development provides the relevant background. Emphasis is given to prevailing silvicultural practices. The genetic resources in Denmark will, in general, be conserved in evolutionary conservation stands, in situ or ex situ. For most species a network of conservation stands is required to cover the spectrum of assumed genecological variability. A preliminary genecological zonation, the biology and the distribution of each species have served to estimate the required number and distribution of conservation stands. From 2 to 15 conservation stands are considered adequate for the different species. The estimated number of conservation stands to conserve the genetic variation of the 75 species is approximately 600. Around 500 will be in situ and around 100 ex situ. The total area required is approximately 1800 ha (excluding isolation zones) or 0.4% of the total forest area and 5% of the natural forest area in Denmark. The size of the area corresponds approximately to the area of certified seed stands/seed production areas in Denmark. The conservation of genetic resources is not pursued by imposing restrictions on the use of the genetic material. The philosophy of the strategy is to effect an insurance and keep all options open. The wise use of the genetic resources will be encouraged by other means. Where target species can be identified and (gene) ecological zones defined, the approach described may be used elsewhere to provide a quick and realistic overview of conservation needs and costs.