Erik Fløjgaard Kristensen

The impact of polychaete (Nereis virens Sars) burrows on nitrification and nitrate reduction in estuarine sediments

Abstract The influence of Nereis virens Sars burrows on nitrification, denitrification and total nitrate reduction was assessed in poor (0.7% organic matter) and rich (2.0% organic matter) sediment from the estuary, Norsminde Fjord. The experiments were performed as assays of potential activity, since natural conditions proved impossible to simulate in the unpredictable burrow environment. The measurements were made in two microprofiles, extending 15 mm into the sediment from the surface and from the burrow wall lining. Both sediment types showed higher potential nitrification in the wall linings than in the surface sediment. This was positively correlated with the content of silt + clay particles and organic matter (i.e. the mucous lining of burrow walls). An elevated nitrate reduction activity was evident in the oxic layer of surface sediment. No such activity pattern was observed in the burrow walls. Denitrification accounted for 27–53 % of the total nitrate reduction. An empirical relationship between the ratio of predicted oxygen penetration into surface and wall sediment and the contribution of nereid burrows to bulk actual nitrification and nitrate reduction is presented. The burrow contribution to bulk nitrification was, in contrast to bulk nitrate reduction, very sensitive to variable oxygen penetrations. Thus, possible short-time changes in nitrate exchange across the wall lining will apparently be regulated by changes in nitrification activity rather than nitrate reduction activity.

Effect of natural concentrations on nutrient exchange between a polychaete burrow in estuarine sediment and the overlying water

The exchange of inorganic nutrients; ammonium, nitrate and reactive phosphate between burrows of the infaunal polychaete Nereis virens Sars and the overlying water was assessed using V-shaped sediment cores. Exchange was determined by monitoring ventilation current and nutrient concentration of in- and excurrent water. Ammonium supply appeared independent of overlying water concentrations, showing a constant release of 0.5 μmol·h−1 (for a 2-g individual + burrow system) at concentrations from 2 to 87 μM. Of this release ≈40% originated from worm excretion, and the rest from microbial mineralization. Nitrate and phosphate exchange appeared very sensitive to overlying water concentrations, having equilibrium (zero flux) at 10–15 and 3 μM, respectively. Below these concentrations nitrate showed a slight release (due to nitrification), whereas phosphate was released at a rate of 3.2 × 10−2 μmol·h−1 at 1 μM (mineralization and desorption). Above equilibrium they both were removed during water passage through worm burrows, reaching 0.4 μmol·h−1 for nitrate at 107 μM (nitrate reduction) and 3.7 × 10−2 μmol·h−1 for phosphate at 5.6 μM (adsorption processes). The burrow system apparently acted as a buffer for phosphate and, to some degree, nitrate in the overlying water. At the study site (Norsminde Fjord estuary) nereid burrows were estimated to increase the sediment-water interface 150%. About 17% of the water column was cycled through the sediment by Nereis each day. The worm + burrow system was estimated to release 95 μmol· m−2·h−1 ammonium to the overlying water, which was ≈76–90% of the total release of ammonium from the sediment (30–36% was worm excretion).

Direct measurement of ventilation and oxygen uptake in three species of tubicolous polychaetes (Nereis spp.)

SummaryThe ventilation of three species ofNereis: N. virens, N. succinea andN. diversicolor are measured by a direct flowsensing technique. The weight dependence of ventilation suggests for all three species a direct proportionality (Fig. 3). Oxygen uptake during active ventilation is approximately 6 times higher than at rest for all three species (Fig. 4). This great difference is assumed a consequence of a low conversion efficience and a high degree of internal work, since only 0.1–0.3‰ of the metabolic increase during activity is transformed to the movement of water in polyethylene tubes (Table 3).