Erik Leeming Kvam
General Electric
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Publication
Featured researches published by Erik Leeming Kvam.
Antimicrobial Agents and Chemotherapy | 2012
Erik Leeming Kvam; Brian Michael Davis; Frank J. Mondello; Allen Lawrence Garner
ABSTRACT Plasma, a unique state of matter with properties similar to those of ionized gas, is an effective biological disinfectant. However, the mechanism through which nonthermal or “cold” plasma inactivates microbes on surfaces is poorly understood, due in part to challenges associated with processing and analyzing live cells on surfaces rather than in aqueous solution. Here, we employ membrane adsorption techniques to visualize the cellular effects of plasma on representative clinical isolates of drug-resistant microbes. Through direct fluorescent imaging, we demonstrate that plasma rapidly inactivates planktonic cultures, with >5 log10 kill in 30 s by damaging the cell surface in a time-dependent manner, resulting in a loss of membrane integrity, leakage of intracellular components (nucleic acid, protein, ATP), and ultimately focal dissolution of the cell surface with longer exposure time. This occurred with similar kinetic rates among methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, and Candida albicans. We observed no correlative evidence that plasma induced widespread genomic damage or oxidative protein modification prior to the onset of membrane damage. Consistent with the notion that plasma is superficial, plasma-mediated sterilization was dramatically reduced when microbial cells were enveloped in aqueous buffer prior to treatment. These results support the use of nonthermal plasmas for disinfecting multidrug-resistant microbes in environmental settings and substantiate ongoing clinical applications for plasma devices.
Archive | 2011
Zaeem Ashraf Khan; Aaron Joseph Dulgar-Tulloch; Stefan Rakuff; Philip Alexander Shoemaker; Erik Leeming Kvam; Xiaohui Chen; Jaydeep Roy
Archive | 2013
Bing Li; David Roger Moore; Erik Leeming Kvam
Archive | 2012
Brian Christopher Bales; Erik Leeming Kvam; Jason Louis Davis
Archive | 2015
John Richard Nelson; David Roger Moore; Robert Scott Duthie; Matthew Jeremiah Misner; Gregory Andrew Grossmann; Elizabeth Marie Dees; Patrick McCoy Spooner; Erik Leeming Kvam; Andrew Burns; Vicki Herzl Watkins
Lab on a Chip | 2015
Craig Patrick Galligan; Jason Michael Nichols; Erik Leeming Kvam; Patrick McCoy Spooner; Rachel Marie Gettings; Li Zhu; Christopher Michael Puleo
Archive | 2013
Bing Li; Gregory Andrew Grossmann; Erik Leeming Kvam; Brian Christopher Bales; Jason Louis Davis
Archive | 2015
Ryan Charles Heller; John Richard Nelson; Erik Leeming Kvam
Archive | 2014
Christopher Michael Puleo; Erik Leeming Kvam; Gregory Andrew Grossmann; Craig Patrick Galligan; Jason Michael Nichols; Xuefeng Wang; Jason Louis Davis
Archive | 2014
Ernest William Kovacs; Erik Leeming Kvam; Bing Li; Frank J. Mondello