Erika Seki Kioshima
Universidade Estadual de Maringá
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Publication
Featured researches published by Erika Seki Kioshima.
Molecules | 2014
Melyssa Negri; Tânia Pereira Salci; Cristiane Suemi Shinobu-Mesquita; Isis Regina Grenier Capoci; Terezinha I.E. Svidzinski; Erika Seki Kioshima
Nosocomial infections caused by fungi have increased greatly in recent years, mainly due to the rising number of immunocompromised patients. However, the available antifungal therapeutic arsenal is limited, and the development of new drugs has been slow. Therefore, the search for alternative drugs with low resistance rates and fewer side effects remains a major challenge. Plants produce a variety of medicinal components that can inhibit pathogen growth. Studies of plant species have been conducted to evaluate the characteristics of natural drug products, including their sustainability, affordability, and antimicrobial activity. A considerable number of studies of medicinal plants and alternative compounds, such as secondary metabolites, phenolic compounds, essential oils and extracts, have been performed. Thus, this review discusses the history of the antifungal arsenal, surveys natural products with potential antifungal activity, discusses strategies to develop derivatives of natural products, and presents perspectives on the development of novel antifungal drug candidates.
Journal of Nano Research | 2014
L. F. Cótica; V. F. Freitas; Daniel Matos Silva; Karina Honjoya; Karen Honjoya; I. A. Santos; Verusca Cristina Pizzatto Fontanive; Rubiana Mara Mainardes; Erika Seki Kioshima; Ruyan Guo; A. S. Bhalla
In the search to reduce the side effects, toxicity and assuring the desired effectiveness of the drugs, many efforts has been made to improve specific drugs’ delivery characteristics. Several carrier nanoparticles have been used to assist the drugs incorporation, absorption and transport through the bloodstream. However, most chemical synthesis routes are multistep and time-consuming treatments and, also, many carrier nanoparticles have toxic effects. In this work, we report a simple one-pot approach for the synthesis of CoFe2O4 nanoparticles (20 to 100 nm). The magnetic measurements revealed nanoparticles with a magnetic saturation nearly one third of that for bulk CoFe2O4. In vitro assays showed no hemolytic potential and negligible toxicity. By in vivo experiments using adult male mice we found no potential risk alterations by the nanoparticles administration. Therefore, the CoFe2O4 nanoparticles, synthesized by the current approach, can be a model drug-carrier, which makes them useful for the biomedical applications.
Journal of Medical Microbiology | 2017
Daniella Renata Faria; Karina Mayumi Sakita; Luciene Akimoto-Gunther; Erika Seki Kioshima; Terezinha Inez Estivalet Svidzinski; Patrícia de Souza Bonfim-Mendonça
The present study aimed to characterize cell damage caused by vaginal Candida albicans isolates from women with different symptomatologies. It was evaluated 12 clinical isolates of C. albicans from vaginal samples: 4 from asymptomatic women (AS), 4 from women with a single episode of vulvovaginal candidiasis (VVC) and 4 from women with recurrent vulvovaginal candidiasis (RVVC). We evaluated the ability of C. albicans to adhere to human cervical cancer cells (SiHa), the yeast-SiHa cell interactions and cell damage. All of the clinical isolates presented a high adhesion capacity on SiHa cells. However, clinical isolates from symptomatic women (VVC and RVVC) had higher filamentation after contact (24 h) with SiHa cells and a greater capacity to cause cell damage (>80 %). Clinical isolates from symptomatic women had greater potential to invade SiHa cells, suggesting that they are more pathogenic than AS isolates.
Future Microbiology | 2017
Juliana Galletti; Flávia Tobaldini-Valerio; Sónia Carina Silva; Erika Seki Kioshima; Larissa Trierveiler-Pereira; Marcos Luciano Bruschi; Melyssa Negri; Terezinha Inez Estivalet Svidzinski
AIM The present study evaluated the capacity of three species of Fusarium isolated from onychomycosis to form biofilms and the antibiofilm effect of propolis extract on these biofilms. MATERIALS & METHODS The biofilms and antibiofilm effects were evaluated by quantifying the colony-forming units, mitochondrial metabolic activity assays, total biomass by crystal violet staining and scanning electron microscopy. RESULTS Propolis extract demonstrated significant antibiofilm efficiency on Fusarium spp. isolates and reduced F. solani, F. oxysporum and F. subglutinans mature biofilms. CONCLUSION Propolis extract can be an alternative topical treatment of onychomycosis caused by Fusarium spp.
Revista Do Instituto De Medicina Tropical De Sao Paulo | 2015
Isis Regina Grenier Capoci; Michele Milano da Cunha; Patrícia de Souza Bonfim-Mendonça; Luciana Dias Ghiraldi-Lopes; Lilian Cristiane Baeza; Erika Seki Kioshima; Terezinha Inez Estivalet Svidzinski
Dermatophytosis is a common zoonosis in urban centers. Dogs and cats have played an important role as its disseminators. Environmental decontamination is essential for the prevention of its propagation to humans and animals. However, sanitizers or disinfectants with antifungal activity, currently available, have high toxicity. The present study evaluated the in vitro effects of an extract of citronella (Cymbopogon nardus) on 31 Microsporum canis isolates from animals and home environments. Susceptibility tests were performed based on document M38-A2 (2008) of the Clinical and Laboratory Standards Institute with modifications for natural products. Although susceptibility variation was observed between the fungus tested, the concentrations that inhibited the growth of 50 and 90% of the microorganisms were low (19.5 and 78 µg/mL, respectively). Thus, this citronella extract showed potent fungistatic and fungicide activities against M. canis isolated from animals and home environments. Therefore, it could be an alternative for dermatophytosis prophylaxis in the home environment.
Drug Discovery Today | 2018
Tânia Pereira Salci; Melyssa Negri; Ana Karina R Abadio; Terezinha Inez Estivalet Svidzinski; Erika Seki Kioshima
Invasive fungal infections are a complex challenge throughout the world because of their high incidence, mainly in critically ill patients, and high mortality rates. The antifungal agents currently available are limited; thus, there is a need for the rapid development of new drugs. In silico methods are a modern strategy to explore interactions between new compounds and specific fungal targets, but they depend on precise genetic information. Here, we discuss the main Candida spp. target genes, including information about null mutants, virulence, cytolocalization, co-regulatory genes, and compounds that are related to protein expression. These data will provide a basis for the future in silico development of antifungal drugs.
Letters in Applied Microbiology | 2017
Leticia da Silva Portes; Erika Seki Kioshima; Zoilo Pires de Camargo; Wagner L. Batista; Patricia Xander
Paracoccidioidomycosis (PCM) is a systemic granulomatous disease endemic in Latin America whose aetiologic agents are the thermodimorphic fungi Paracoccidioides brasiliensis and Paracoccidioides lutzii. Despite technological advances, some problems have been reported for the fungal antigens used for serological diagnosis, and inconsistencies among laboratories have been reported. The use of synthetic peptides in the serological diagnosis of infectious diseases has proved to be a valuable strategy because in some cases, the reactions are more specific and sensitive. In this study, we used a subtractive selection with a phage display library against purified polyclonal antibodies for negative and positive PCM sera caused by P. brasiliensis. The binding phages were sequenced and tested in a binding assay to evaluate its interaction with sera from normal individuals and PCM patients. Synthetic peptides derived from these phage clones were tested in a serological assay, and we observed a significant recognition of LP15 by sera from PCM patients infected with P. brasiliensis. Our results demonstrated that subtractive phage display selection may be useful for identifying new epitopes that can be applied to the serodiagnosis of PCM caused by P. brasiliensis.
Antimicrobial Agents and Chemotherapy | 2017
Mariane C. Bagatin; Arethusa Lobo Pimentel; Débora Carina Biavatti; Ernani A. Basso; Erika Seki Kioshima; Flavio Augusto Vicente Seixas; Gisele F. Gauze
ABSTRACT This work evaluated new potential inhibitors of the enzyme homoserine dehydrogenase (HSD) of Paracoccidioides brasiliensis, one of the etiological agents of paracoccidioidomycosis. The tertiary structure of the protein bonded to the analogue NAD, and l-homoserine was modeled by homology. The model with the best output was subjected to gradient minimization, redocking, and molecular dynamics simulation. Virtual screening simulations with 187,841 molecules purchasable from the Zinc database were performed. After the screenings, 14 molecules were selected and analyzed by the use of absorption, distribution, metabolism, excretion, and toxicity criteria, resulting in four compounds for in vitro assays. The molecules HS1 and HS2 were promising, exhibiting MICs of 64 and 32 μg · ml−1, respectively, for the Pb18 isolate of P. brasilensis, 64 μg · ml−1 for two isolates of P. lutzii, and also synergy with itraconazole. The application of these molecules to human-pathogenic fungi confirmed that the HSD enzyme may be used as a target for the development of drugs with specific action against paracoccidioidomycosis; moreover, these compounds may serve as leads in the design of new antifungals.
Expert Opinion on Drug Discovery | 2014
Zanni Pc; Melyssa Negri; Tânia Pereira Salci; Bonfim-Mendonça Pde S; Erika Seki Kioshima; Svidzinski TIe; Marcia Edilaine Lopes Consolaro
Introduction: Vaginal atrophy (VA) is an inflammation of the vagina that develops when there is a significant decrease in levels of the estrogen. Prolonged periods of hypoestrogenism may induce severe VA and treatment is essential. This is a significant problem which requires more focused attention for the development of existing and future therapies. Areas covered: This review evaluates the suitable animal models of VA, including: mice, rodents and non-human primates. It focuses particularly on the possibilities and limitations of these in vivo models for the effective development of VA therapies. Expert opinion: Hormone replacement therapy (HRT) has been prescribed and successfully used for VA. However, some studies have shown that HRT may be linked to an increased risk of breast cancer, coronary heart diseases and others risks. Thus, there is a growing interest in effective and safe alternatives to VA symptoms. There are, however, a number of things that must be considered for future drug discovery efforts. One major consideration is what animal model should be used and whether the model is appropriate for the study aim. Similarly, research studies must also consider the influencing factors on these animal models, so that these models can effectively mimic the actual disease. The authors also highlight the need to standardize research parameters to produce more reliable and reproducible data.
Anais Brasileiros De Dermatologia | 2002
Ana Carla Pozzi Oliveira; Eliana Guilhermetti; Erika Seki Kioshima; Márcia Rejane Pedra; Terezinha Inez Estivalet Svidzinski