Erin A. Dreelin

INFLUENCE OF LANDSCAPE ELEMENTS ON POPULATION DENSITIES AND HABITAT USE OF THREE SMALL-MAMMAL SPECIES

Abstract Corridor effects on population densities and habitat use of 3 small-mammal species were assessed during 1998–2000 in an experimentally fragmented landscape. Corridor presence did not have a statistically significant effect on population densities of cotton rats (Sigmodon hispidus) or cotton mice (Peromyscus gossypinus); however, a significant effect was observed for old-field mice (Peromyscus polionotus) during 2000. Cotton rats were captured more frequently than expected in corridors, while old-field mice were captured more frequently than expected in habitat-patch interior; and cotton mice exhibited a more uniform distribution across habitat types. These results suggest that landscape fragmentation and habitat structure may have varying effects on population densities of different species.

Coupling socioeconomic and lake systems for sustainability: a conceptual analysis using Lake St. Clair region as a case study.

Applying sustainability at an operational level requires understanding the linkages between socioeconomic and natural systems. We identified linkages in a case study of the Lake St. Clair (LSC) region, part of the Laurentian Great Lakes system. Our research phases included: (1) investigating and revising existing coupled human and natural systems frameworks to develop a framework for this case study; (2) testing and refining the framework by hosting a 1-day stakeholder workshop and (3) creating a causal loop diagram (CLD) to illustrate the relationships among the systems’ key components. With stakeholder assistance, we identified four interrelated pathways that include water use and discharge, land use, tourism and shipping that impact the ecological condition of LSC. The interrelationships between the pathways of water use and tourism are further illustrated by a CLD with several feedback loops. We suggest that this holistic approach can be applied to other case studies and inspire the development of dynamic models capable of informing decision making for sustainability.

Cryptosporidium and Giardia in Surface Water: A Case Study from Michigan, USA to Inform Management of Rural Water Systems

Cryptosporidium and Giardia pose a threat to human health in rural environments where water supplies are commonly untreated and susceptible to contamination from agricultural animal waste/manure, animal wastewater, septic tank effluents and septage. Our goals for this paper are to: (1) explore the prevalence of these protozoan parasites, where they are found, in what quantities, and which genotypes are present; (2) examine relationships between disease and land use comparing human health risks between rural and urban environments; and (3) synthesize available information to gain a better understanding of risk and risk management for rural water supplies. Our results indicate that Cryptosporidium and Giardia were more prevalent in rural versus urban environments based on the number of positive samples. Genotyping showed that both the human and animal types of the parasites are found in rural and urban environments. Rural areas had a higher incidence of disease compared to urban areas based on the total number of disease cases. Cryptosporidiosis and giardiasis were both positively correlated (p < 0.001) with urban area, population size, and population density. Finally, a comprehensive strategy that creates knowledge pathways for data sharing among multiple levels of management may improve decision-making for protecting rural water supplies.

Creating a Dialogue for Effective Collaborative Decision-making: A Case Study with Michigan Stakeholders

ABSTRACT Although widely promoted, the importance of science as the basis of natural resources policy-making has proven difficult to both implement and evaluate compared to the many other inputs to creating public policy. Our goals were to bring together a diverse group of stakeholders in Michigan and develop means to create a dialogue on the use of water science in policy-making and to address the disconnect between the science and water policy at the state and regional levels. To achieve these goals, we developed a series of workshops and facilitated discussions to encourage active, productive discussion with a group of 35 Water Resource Fellows (Fellows). The discussions examined the role of science in Michigan water policy. The Fellows were representatives from academia, local governments, state agencies, environmental groups, industry, agriculture, and business. We surveyed the Fellows about their views on the role of science and water issues in Michigan. Nearly half (45%) of the Fellows stated that not enough science is currently being used in water policy decisions. Several themes emerged from the facilitated discussions. The Fellows recommended a recursive decision-making approach to using science in policy making. The Fellows also expressed the need for science to be accessible, relevant to the policy community, and communicated to regulators and the general public on an ongoing basis. The workshop series process for encouraging discussion among stakeholders can be used in other collaborative decision-making efforts.

Effective Cross-Border Monitoring Systems for Waterborne Microbial Pathogens

This book provides a real-world analysis of how to quantify and prioritize water-based microbial threats to human health, how to design data collection systems that truly support management decisions, and how to build a comprehensive monitoring program when dealing with cross-boundary issues. International borders and cross-boundary issues complicate water resource management. Even if nations or jurisdictions agree on the nature and source of the problem, differing legal frameworks complicate cross-border management, as the procedural steps and time necessary to implement a solution vary among entities. Waterborne pathogens transcend political boundaries and challenge the use of traditional political jurisdictions in meeting public policy objectives to protect human health. Disease outbreaks caused by waterborne pathogens continue to occur, even in the developed world. There has been a pronounced trend toward new and increasingly complex institutions and policies to address regional water quality management issues. However, many questions continue to arise regarding the effective management of regional resources. These questions include how to design effective monitoring strategies and what can be learned from previous successes and failures. The Laurentian Great Lakes offers a model system for exploring these questions. This book will be a valuable reference source for researchers and graduate students working in environmental science, microbiology, engineering, and biological sciences, as well as all those concerned with water quality monitoring programs.

Isothermal amplification of environmental DNA (eDNA) for direct field-based monitoring and laboratory confirmation of Dreissena sp.

Loop-mediated isothermal amplification (LAMP) of aquatic invasive species environmental DNA (AIS eDNA) was used for rapid, sensitive, and specific detection of Dreissena sp. relevant to the Great Lakes (USA) basin. The method was validated for two uses including i) direct amplification of eDNA using a hand filtration system and ii) confirmation of the results after DNA extraction using a conventional thermal cycler run at isothermal temperatures. Direct amplification eliminated the need for DNA extraction and purification and allowed detection of target invasive species in grab or concentrated surface water samples, containing both free DNA as well as larger cells and particulates, such as veligers, eggs, or seeds. The direct amplification method validation was conducted using Dreissena polymorpha and Dreissena bugensis and uses up to 1 L grab water samples for high target abundance (e.g., greater than 10 veligers (larval mussels) per L for Dreissena sp.) or 20 L samples concentrated through 35 μm nylon screens for low target abundance, at less than 10 veligers per liter water. Surface water concentrate samples were collected over a period of three years, mostly from inland lakes in Michigan with the help of a network of volunteers. Field samples collected from 318 surface water locations included i) filtered concentrate for direct amplification validation and ii) 1 L grab water sample for eDNA extraction and confirmation. Though the extraction-based protocol was more sensitive (resulting in more positive detections than direct amplification), direct amplification could be used for rapid screening, allowing for quicker action times. For samples collected between May and August, results of eDNA direct amplification were consistent with known presence/absence of selected invasive species. A cross-platform smartphone application was also developed to disseminate the analyzed results to volunteers. Field tests of the direct amplification protocol using a portable device (Gene-Z) showed the method could be used in the field to obtain results within one hr (from sample to result). Overall, the direct amplification has the potential to simplify the eDNA-based monitoring of multiple aquatic invasive species. Additional studies are warranted to establish quantitative correlation between eDNA copy number, veliger, biomass or organismal abundance in the field.