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Dive into the research topics where Erkang Wang is active.

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Featured researches published by Erkang Wang.


ACS Nano | 2011

Hemin-Graphene Hybrid Nanosheets with Intrinsic Peroxidase-like Activity for Label-free Colorimetric Detection of Single-Nucleotide Polymorphism

Yujing Guo; Liu Deng; Jing Li; Shaojun Guo; Erkang Wang; Shaojun Dong

This paper demonstrated for the first time a simple wet-chemical strategy for synthesizing hemin-graphene hybrid nanosheets (H-GNs) through the π-π interactions. Significantly, this new material possesses the advantages of both hemin and graphene and exhibits three interesting properties. First, H-GNs have intrinsic peroxidase-like activity, which can catalyze the reaction of peroxidase substrate, due to the existence of hemin on the graphene surface. Second, their dispersion follow the 2D Schulze-Hardy rule, that is to say, the coagulation of H-GNs in electrolyte solution results from the interplay between van der Waals attraction and electric double-layer repulsion. Third, H-GNs exhibit the ability to differentiate ss- and ds-DNA in optimum electrolyte concentration, owing to the different affinities of ss- and ds-DNA to the H-GNs. On the basis of these unique properties of the as-prepared H-GNs, we have developed a label-free colorimetric detection system for single-nucleotide polymorphisms (SNPs) in disease-associated DNA. To our knowledge, this is the first report concerning on SNPs detection using functionalized graphene nanosheets. Owing to its easy operation and high specificity, it was expected that the proposed procedure might hold great promise in the pathogenic diagnosis and genetic diseases.


Biosensors and Bioelectronics | 2001

Amplification of antigen–antibody interactions based on biotin labeled protein–streptavidin network complex using impedance spectroscopy

Renjun Pei; Zhiliang Cheng; Erkang Wang; Xiurong Yang

Antibody was covalently immobilized by amine coupling method to gold surfaces modified with a self-assembled monolayer of thioctic acid. The electrochemical measurements of cyclic voltammetry and impedance spectroscopy showed that the hexacyanoferrate redox reactions on the gold surface were blocked due to the procedures of self-assembly of thioctic acid and antibody immobilization. The binding of a specific antigen to antibody recognition layer could be detected by measurements of the impedance change. A new amplification strategy was introduced for improving the sensitivity of impedance measurements using biotin labeled protein-streptavidin network complex. This amplification strategy is based on the construction of a molecular complex between streptavidin and biotin labeled protein. This complex can be formed in a cross-linking network of molecules so that the amplification of response signal will be realized due to the big molecular size of complex. The results show that this amplification strategy causes dramatic improvement of the detection sensitivity of hIgG and has good correlation for detection of hIgG in the range of 2-10 microg/ml.


Proceedings of the National Academy of Sciences of the United States of America | 2011

Quantifying the Waddington landscape and biological paths for development and differentiation.

Jin Wang; Kun Zhang; Li Xu; Erkang Wang

We developed a theoretical framework to prove the existence and quantify the Waddington landscape as well as chreode-biological paths for development and differentiation. The cells can have states with the higher probability ones giving the different cell types. Different cell types correspond to different basins of attractions of the probability landscape. We study how the cells develop from undifferentiated cells to differentiated cells from landscape perspectives. We quantified the Waddington landscape through construction of underlying probability landscape for cell development. We show the developmental process proceeds as moving from undifferentiated to the differentiated basins of attractions. The barrier height of the basins of attractions correlates with the escape time that determines the stability of cell types. We show that the developmental process can be quantitatively described and uncovered by the biological paths on the quantified Waddington landscape from undifferentiated to the differentiated cells. We found the dynamics of the developmental process is controlled by a combination of the gradient and curl force on the landscape. The biological paths often do not follow the steepest descent path on the landscape. The landscape framework also quantifies the possibility of reverse differentiation process such as cell reprogramming from differentiated cells back to the original stem cell. We show that the biological path of reverse differentiation is irreversible and different from the one for differentiation process. We found that the developmental process described by the underlying landscape and the associated biological paths is relatively stable and robust against the influences of environmental perturbations.


Biosensors and Bioelectronics | 2001

Capacitive detection of glucose using molecularly imprinted polymers

Zhiliang Cheng; Erkang Wang; Xiurong Yang

A novel glucose biosensor based on capacitive detection has been developed using molecularly imprinted polymers. The sensitive layer was prepared by electropolymerization of o-phenylenediamine on a gold electrode in the presence of the template (glucose). Cyclic voltammetry and capacitive measurements monitored the process of electropolymerization. Surface uncovered areas were plugged with 1-dodecanethiol to make the layer dense, and the insulating properties of the layer were studied in the presence of redox couples. The template molecules and the nonbound thiol were removed from the modified electrode surface by washing with distilled water. A capacitance decrease could be obtained after injection of glucose. The electrode constructed similarly but with ascorbic acid or fructose only showed a small response compared with glucose. The stability and reproducibility of the biosensor were also investigated.


ACS Nano | 2011

Ion-Tuned DNA/Ag Fluorescent Nanoclusters As Versatile Logic Device

Tao Li; Libing Zhang; Jun Ai; Shaojun Dong; Erkang Wang

A novel kind of versatile logic device has been constructed utilizing ion-tuned DNA/Ag fluorescent nanoclusters, with K(+) and H(+) as two inputs. A well-chosen hairpin DNA with a poly-C loop serves as the template for synthesizing two species of Ag nanoclusters. Several G-tracts and C-tracts on its two terminals enable the hairpin DNA to convert into the G-quadruplex and/or i-motif structures upon input of K(+) and H(+). Such a structural change remarkably influences the spectral behaviors of Ag nanoclusters. In particular, different species of Ag nanoclusters have distinct fluorescence responses to the input of K(+) and H(+). These unique features of DNA/Ag nanoclusters enable multiple logic operations via multichannel fluorescence output, indicating the versatility as a molecular logic device. By altering the specific sequence of the hairpin DNA, more logic gates can be constructed utilizing Ag nanoclusters.


Accounts of Chemical Research | 2011

Functional Micro/Nanostructures: Simple Synthesis and Application in Sensors, Fuel Cells, and Gene Delivery

Shaojun Guo; Erkang Wang

In order to develop new, high technology devices for a variety of applications, researchers would like to better control the structure and function of micro/nanomaterials through an understanding of the role of size, shape, architecture, composition, hybridization, molecular engineering, assembly, and microstructure. However, researchers continue to face great challenges in the construction of well-defined micro/nanomaterials with diverse morphologies. At the same time, the research interface where micro/nanomaterials meet electrochemistry, analytical chemistry, biomedicine, and other fields provides rich opportunities to reveal new chemical, physical, and biological properties of micro/nanomaterials and to uncover many new functions and applications of these materials. In this Account, we describe our recent progress in the construction of novel inorganic and polymer nanostructures formed through different simple strategies. Our synthetic strategies include wet-chemical and electrochemical methods for the controlled production of inorganic and polymer nanomaterials with well-defined morphologies. These methods are both facile and reliable, allowing us to produce high-quality micro/nanostructures, such as nanoplates, micro/nanoflowers, monodisperse micro/nanoparticles, nanowires, nanobelts, and polyhedron and even diverse hybrid structures. We implemented a series of approaches to address the challenges in the preparation of new functional micro/nanomaterials for a variety of important applications This Account also highlights new or enhanced applications of certain micro/nanomaterials in sensing applications. We singled out analytical techniques that take advantage of particular properties of micro/nanomaterials. Then by rationally tailoring experimental parameters, we readily and selectively obtained different types of micro/nanomaterials with novel morphologies with high performance in applications such as electrochemical sensors, electrochemiluminescent sensors, gene delivery agents, and fuel cell catalysts. We expect that micro/nanomaterials with unique structural characteristics, properties, and functions will attract increasing research interest and will lead to new opportunities in various fields of research.


Biochimica et Biophysica Acta | 2002

Direct electron transfer between hemoglobin and a glassy carbon electrode facilitated by lipid-protected gold nanoparticles

Xiaojun Han; Wenlong Cheng; Zheling Zhang; Shaojun Dong; Erkang Wang

We synthesized a kind of gold nanoparticle protected by a synthetic lipid (didodecyldimethylammonium bromide, DDAB). With the help of these gold nanoparticles, hemoglobin can exhibit a direct electron transfer (DET) reaction. The formal potential locates at -169 mV vs. Ag/AgCl. Spectral data indicated the hemoglobin on the electrode was not denatured. The lipid-protected gold nanoparticles were very stable (for at least 8 months). Their average diameter is 6.42 nm. It is the first time to use monolayer-protected nanoparticles to realize the direct electrochemistry of protein.


Electrophoresis | 2002

New technique for capillary electrophoresis directly coupled with end‐column electrochemiluminescence detection

Weidong Cao; Jifeng Liu; Xiurong Yang; Erkang Wang

A new end‐column electrochemiluminescence (ECL) detection technique coupling to capillary electrophoresis (CE) is characterized. A 300 νm diameter Pt working electrode was used to directly couple with a 75 νm inner diameter separation capillary without an electric field decoupler. The hydrodynamic cyclic voltammogram (CV) of Ru(bpy)32+ showed that electrophoretic current did not affect the ECL reaction. The presence of high‐voltage (HV) field only resulted in the shift of the ECL detection potential. The distance of capillary to electrode was an important parameter for optimizing detection performance as it determined the characteristics of mass transport toward the electrode and the actual concentration of Ru(bpy)32+ in the detection region. The optimum distance of capillary to electrode was decided by the inner diameter of the capillary, too. For a 75 νm capillary, the working electrode should be placed away from the capillary outlet at a distance within the range of 220–260 νm. The effects of pH value of ECL solution and molecular structure of analytes on peak height and theoretical plate numbers were discussed. Using the 75 νm capillary, under the optimum conditions, the method provided a linear range for tripropylamine (TPA) between 1×10−10 and 1×10−5 mol/L with correlation coefficient of 0.998. The detection limit (signal‐to‐noise ratio S/N = 3) was 5.0×10−11 mol/L. The relative standard deviation in peak height for eight consecutive injections was 5.6%. By this new technique lidocaine spiked in a urine sample was determined. The method exhibited the linear range for lidocaine from 5.0×10−8 to 1.0×10−5 mol/L with correlation efficient of 0.998. The limit of detection (S/N = 3) was 2.0×10−8 mol/L.


Analytical Chemistry | 2011

A nanoparticle autocatalytic sensor for Ag+ and Cu2+ ions in aqueous solution with high sensitivity and selectivity and its application in test paper.

Xuan Yang; Erkang Wang

A novel nanoparticle autocatalytic sensor for the detection of Ag(+) and Cu(2+) has been constructed based on the oxidative ability of Ag(+) and Cu(2+) toward o-phenylenediamine (OPDA). Ag(+) and Cu(2+) can be reduced to zerovalent silver and copper, respectively, and then such zerovalent Ag and Cu species form silver and copper nanoparticles that can catalyze the reaction between OPDA and Ag(+) and Cu(2+). In the reaction, OPDA is oxidized to 2,3-diaminophenazine (OPDAox), which has a fluorescence emission at 568 nm. Under the optimal conditions, Ag(+) and Cu(2+) can be detected in the concentration ranges from 60 nM to 60 μM and from 2.5 nM to 25 μM, respectively. Through this facile approach, Ag(+) and Cu(2+) can be detected down to 60 nM and 2.5 nM, respectively. In addition, the sensor is utilized for the detection of Ag(+) and Cu(2+) in sewage, and we have obtained very good results that are consistent with those of inductively coupled plasma-mass spectroscopy (ICP-MS). Moreover, such a nanoparticle autocatalytic sensor is applied to test paper for the detection of Ag(+) and Cu(2+) with the naked eye. With such test paper, Ag(+) and Cu(2+) could be detected at levels as low as 0.06 nmol and 0.3 nmol, respectively, with detection ranges of 0.06-60 nmol for Ag(+) and 0.3-60 nmol for Cu(2+), under the irradiation of UV light (365 nm). The test paper could be potentially used in the rapid detection of Ag(+) and Cu(2+) in real samples.


Journal of Chromatography A | 2003

Determination of biogenic amines by capillary electrophoresis with pulsed amperometric detection

Xiuhua Sun; Xiurong Yang; Erkang Wang

The biogenic amines, putrescine, cadaverine, spermidine and spermine were separated and quantified by capillary electrophoresis with pulsed amperometric detection. Detection potential of the pulsed amperometric detection was optimized as 0.6 V. Optimal separation of the biogenic amines was achieved using a separation buffer of 30 mM citrate at pH 3.5, while keeping the buffer in the detection cell as 20 mM NaOH. Using these conditions, the four biogenic amines were baseline separated. Extrapolated limits of detection for putrescine, cadaverine, spermidine and spermine were 400, 200, 100 and 400 nM for the standard mixture (polyamines dissolved in running buffer), respectively. These are lower than ultraviolet detection and comparable or even lower than laser-induced fluorescence detection results as reported in the literature. The number of theoretical plates was maintained at the 10(5) level, which is absolutely higher than any reported method. When applying capillary electrophoresis-pulsed amperometric detection to milk analysis, only spermidine was found in amounts varying between 0.1 and 0.5 mg/kg.

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Shaojun Dong

Chinese Academy of Sciences

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Xiurong Yang

Chinese Academy of Sciences

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Xue-Bo Yin

Chinese Academy of Sciences

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Xiaojun Han

Chinese Academy of Sciences

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Jifeng Liu

Chinese Academy of Sciences

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Jilin Tang

Chinese Academy of Sciences

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Yan Du

Chinese Academy of Sciences

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Shaojun Guo

Chinese Academy of Sciences

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Tao Li

University of Science and Technology of China

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