Erni Suminar

Pemberdayaan Wanita Tani dalam Budidaya Kedelai Varietas Unggul Bahan Baku Pembuatan Tahu

WOMEN FARMERS EMPOWERMENT IN SOYBEAN CULTIVATION OF SUPERIOR VARIETIES RAW MATERIAL OF TOFU PRODUCTION. Soybean is one of the important crops containing high protein content. The demand of soybean increases from year to year, because the domestic production could not reach the market demand and it is still imported. Therefore, the necessary effort to increase soybean production must be done such as by expanding the planting area, planting superior varieties especially for raw materials of tofu, and the cultivation techniques. Sumedang is one of the centers of tofu production in West Java that has the suitable agro-climatic conditions for soybeanproduction as a raw material. Some of the farm communities have cultivated soybeans, however they still needs the efforts to increase the soybean production especially for the types of soybean for raw material of tofu production. The aim of IbM activity is to socialize and make a pilot demonstration plot of some of superior soybean varieties for tofu production in Sumedang. The results of these activities include: (1) the demonstration plots of soybean varieties, (2) producing the high seed quality of superior soybean varieties, (3) improving skill and knowledge of farmers in the cultivation of superior soybean varieties and also pest and disease control, (4) obtaining the samples of tofu from superior soybean varieties grown in partner locations.

Pengujian berbagai eksplan kentang (Solanum tuberosum L.) dengan penggunaan konsentrasi BAP dan NAA yang berbeda

Rendahnya produksi kentang di Indonesia disebabkan oleh ketersediaan benih kentang bermutu hasil dari metode konvensional yang kurang memadai. Metode kultur jaringan mampu menghasilkan bibit bermutu dan bebas virus, dalam jumlah banyak serta waktu yang singkat. Penelitian ini bertujuan untuk mendapatkan konsentrasi 6-benzylaminopurine (BAP) dan Naphthalene Acetic Acid (NAA) terbaik pada berbagai eksplan untuk pertumbuhan tunas meriklon kentang (Solanum tuberosum L.) Kultivar Atlantik secara in vitro. Percobaan dilaksanakan pada November 2016 sampai Februari 2017 di Laboratorium Kultur Jaringan Teknologi Benih Fakultas Pertanian Universitas Padjadjaran. Rancangan Acak Lengkap (RAL) digunakan sebagai rancangan percobaan yang terdiri dari dua unit percobaan terpisah yaitu unit eksplan meristem interkalar dan meristem apikal, masing-masing delapan perlakuan dan empat ulangan. Media Murashige and Skoog (MS) digunakan sebagai media dasar dengan penambahan BAP (0,0 mg L -1 ; 1 mg L -1 ; 1,5 mg L -1 ; 2 mg L -1 ) dan NAA (0,0 mg L -1 ; 0,5 mg L -1 ). Hasil menunjukkan konsentrasi BAP 1 mg L -1 merupakan perlakuan yang paling baik dalam menghasilkan jumlah tunas, cabang, daun dan buku pada eksplan meristem interkalar. Pada eksplan meristem apikal, penambahan BAP dan NAA belum mampu meningkatkan pertumbuhan eksplan. Meristem interkalar yang diberi auksin dan sitokinin berpotensi menjadi bahan alternatif eksplan meriklon kentang selain meristem apikal. The production of potato in Indonesia is low due to the limited number of high quality potato seeds produced from the conventional methods. The tissue culture methods can be used to produce high quality and virus-free seeds in more reliable number in a short time. Randomized Completely Design (RCD) was used as the experimental design consisted of two separate trial units namely intercalary meristem and apical meristem explants units with eight treatments and four replications of each. Murashige and Skoog (MS) was used as the base media added with BAP i.e. 0.0 mg L -1 ; 1 mg L -1 ; 1.5 mg L -1 ; 2 mg L -1 and NAA 0.0 mg L -1 and 0.5 mg L -1 . Results showed that concentration of BAP 1 mg L -1 generated the best results on the number of shoots, branches, leaves and nodes on meristem intercalary explant. Meanwhile on apical meristem explant, the addition of BAP and NAA had not yet improved the explant growth. Intercalary meristem added with auxin and cytokinin is potential as alternative of mericlone explants material of potato besides the apical meristem.

The Growth and Cultivation of Meristem From Shallot (Allium ascalonicum L.) cv. Katumi by In Vitro

This study aimed for knowing and obtaining the best concentration of kinetin and NAA interaction effects in influencing the shoot induction, knowing how the plant growth regulators in induction mediastill affect the shoot additionin the MS 0 media and also knowing the largest number of roots in rooting media for shallot by in vitro . The experiment was conducted at Laboratory of Tissue Culture Seed Technology, Faculty of Agriculture, Padjadjaran University, during January 2011 until May 2011. This experiment divided in 3 stages, namely shoot induction stage, shoot subculture to MS 0 media stage and shoot subculture to rooting media stage. Experimental method used in the shoot induction stage was factorial Completely Randomized Design with three replications. The first factor was the kinetin with four levels,0, 1, 2, and 3 mg L -1. The second factor was the NAA with three levels, as 0, 0.01, and 0.1 mg L -1 . Basic media used for each treatment was MS. The experiment result showed there was an interaction between kinetin and NAA on shoot induction stagewith the plantlet height, leaf number, and shoot addition. The best result for leaf number was gained from interaction with 2 mg L -1 kinetin without NAA,while the treatment of 2 mg L -1 kinetin with 0.01 mg L -1 NAA gave a better interaction for theshoot addition variable.