Erol Alver

One step effective removal of Congo Red in chitosan nanoparticles by encapsulation

Chitosan nanoparticles (CNPs) were prepared with ionotropic gelation between chitosan and tripolyphosphate for the removal of Congo Red. The production of chitosan nanoparticles and the dye removal process was carried out in one-step. The removal efficiency of Congo Red by encapsulation within chitosan from the aqueous solution and its storage stability are examined at different pH values. The influence of some parameters such as the initial dye concentration, pH value of the dye solution, electrolyte concentration, tripolyphosphate concentration, mixing time and speed on the encapsulation is examined. Congo Red removal efficiency and encapsulation capacity of chitosan nanoparticles were determined as above 98% and 5107mg Congo Red/g chitosan, respectively.

Fabrication and characterization of gold-nanoparticles/chitosan film: a scaffold for L929-fibroblasts

Abstract The objective of the present study was to fabricate a gold nanoparticle crosslinked chitosan (Ch/AuNPs) composite film simple and to evaluate its use as a carrier matrix for L929- fibroblasts. L929- fibroblasts were seeded either onto Ch or Ch/AuNPs scaffolds. The Ch/AuNPs scaffold exhibited a higher cell proliferation and growth rate. The cytotoxicity test determined trypan blue staining indicated that Ch scaffolds devoid of AuNPs expressed almost no toxicity while the Ch/AuNPs composite scaffolds expressed a very limited toxicity only at higher doses. The Ch/AuNPs scaffold promotes cell attachment, growth and proliferation with almost no cytotoxicity.

DETERMINATION OF POLYCYCLIC AROMATIC HYDROCARBONS IN CIGARETTE FILTER TAR BY MEANS OF HOLLOW-FIBER LIQUID PHASE MICROEXTRACTION-HPLC-UV SYSTEM

A high-pressure liquid chromatographic method was developed to determine polycyclic aromatic hydrocarbons (PAHs) in cigarette filter tar using hollow fiber-liquid-phase microextraction (HF-LPME) for the clean-up and enrichment steps. The donor phase for HF-LPME is a mixture of acetonitrile and water. Before analyzing real cigarette samples, some preliminary experiments were necessary to find the optimum starting HF-LPME conditions, including donor phase composition (acetonitrile/water), pH, acceptor phase type, mixing rate, and extraction time. These experiments were performed with model PAH solutions. The chromatographic analysis of the acceptor solution was performed using a C18 reversed-phase column and a UV detector system. Under optimum conditions, percent recoveries and enrichment factors ranged from 63 to 97% and from 208 to 320, respectively, for model PAH solutions. The recoveries from real samples were between 8 and 71%, whereas the enrichment factors ranged from 27 to 234. The limit of detection (LOD) of the six PAHs was found to be between 0.040 and 0.136 ng mL−1. The concentrations of the six PAHs in real samples (various brands of cigarettes) were determined. The total amounts of PAHs (six) found in the filter tar of the three brands filter tar were 165.49, 50.49, and 51.04 ng/cigarette, respectively.