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Dive into the research topics where Esperanza Alonso is active.

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Featured researches published by Esperanza Alonso.


Pflügers Archiv: European Journal of Physiology | 2015

Kv1.3 channels modulate human vascular smooth muscle cells proliferation independently of mTOR signaling pathway

Pilar Cidad; Eduardo Miguel-Velado; Christian Ruiz-McDavitt; Esperanza Alonso; Laura Jiménez-Pérez; Agustín Asuaje; Yamila Carmona; Daniel García-Arribas; Javier López; Yngrid Marroquín; Mirella Fernández; Mercè Roqué; M. Teresa Pérez-García; José R. López-López

Phenotypic modulation (PM) of vascular smooth muscle cells (VSMCs) is central to the process of intimal hyperplasia which constitutes a common pathological lesion in occlusive vascular diseases. Changes in the functional expression of Kv1.5 and Kv1.3 currents upon PM in mice VSMCs have been found to contribute to cell migration and proliferation. Using human VSMCs from vessels in which unwanted remodeling is a relevant clinical complication, we explored the contribution of the Kv1.5 to Kv1.3 switch to PM. Changes in the expression and the functional contribution of Kv1.3 and Kv1.5 channels were studied in contractile and proliferating VSMCs obtained from human donors. Both a Kv1.5 to Kv1.3 switch upon PM and an anti-proliferative effect of Kv1.3 blockers on PDGF-induced proliferation were observed in all vascular beds studied. When investigating the signaling pathways modulated by the blockade of Kv1.3 channels, we found that anti-proliferative effects of Kv1.3 blockers on human coronary artery VSMCs were occluded by selective inhibition of MEK/ERK and PLCγ signaling pathways, but were unaffected upon blockade of PI3K/mTOR pathway. The temporal course of the anti-proliferative effects of Kv1.3 blockers indicates that they have a role in the late signaling events essential for the mitogenic response to growth factors. These findings establish the involvement of Kv1.3 channels in the PM of human VSMCs. Moreover, as current therapies to prevent restenosis rely on mTOR blockers, our results provide the basis for the development of novel, more specific therapies.


Journal of Biological Chemistry | 2016

Molecular Determinants of Kv1.3 Potassium Channels-induced Proliferation.

Laura Jiménez-Pérez; Pilar Cidad; I Alvarez-Miguel; Alba Santos-Hipólito; Rebeca Torres-Merino; Esperanza Alonso; Miguel A. de la Fuente; José R. López-López; M. Teresa Pérez-García

Changes in voltage-dependent potassium channels (Kv channels) associate to proliferation in many cell types, including transfected HEK293 cells. In this system Kv1.5 overexpression decreases proliferation, whereas Kv1.3 expression increases it independently of K+ fluxes. To identify Kv1.3 domains involved in a proliferation-associated signaling mechanism(s), we constructed chimeric Kv1.3-Kv1.5 channels and point-mutant Kv1.3 channels, which were expressed as GFP- or cherry-fusion proteins. We studied their trafficking and functional expression, combining immunocytochemical and electrophysiological methods, and their impact on cell proliferation. We found that the C terminus is necessary for Kv1.3-induced proliferation. We distinguished two residues (Tyr-447 and Ser-459) whose mutation to alanine abolished proliferation. The insertion into Kv1.5 of a sequence comprising these two residues increased proliferation rate. Moreover, Kv1.3 voltage-dependent transitions from closed to open conformation induced MEK-ERK1/2-dependent Tyr-447 phosphorylation. We conclude that the mechanisms for Kv1.3-induced proliferation involve the accessibility of key docking sites at the C terminus. For one of these sites (Tyr-447) we demonstrated the contribution of MEK/ERK-dependent phosphorylation, which is regulated by voltage-induced conformational changes.


Journal of the American College of Cardiology | 2015

ROLE OF KV1.3 CHANNEL IN VASCULAR SMOOTH MUSCLE CELLS PROLIFERATION IN A PORCINE MODEL OF RESTENOSIS

Mercè Roqué; Manel Garabito; Solanes Nuria; Montserrat Rigol; Pilar Cidad; M. Teresa Pérez-García; Victor Ramos; José Ramón López; Salvador Borrós; Esperanza Alonso; Laura Jimenez Perez

Vascular smooth muscle cells (VSMCs) switch from a contractile to a proliferative phenotype, in response to arterial injury, such as after percutaneous coronary interventions (PCI). This phenotypic switch implies a profound change in the expression of contractile proteins and ion channels. In a


Archive | 2004

GENETIC AND GEOGRAPHIC VARIATION IN SEED TRAITS OF COMMON WALNUT AMONG TWENTY POPULATIONS FROM THE WEST OF SPAIN

Rocío Díaz; Esperanza Alonso; J Fernandez Lopez


Space Science Reviews | 2018

ExoMars Trace Gas Orbiter (TGO) Science Ground Segment (SGS)

Leo Metcalfe; M. Aberasturi; Esperanza Alonso; Rosa Alvarez; Mike Ashman; Isa Barbarisi; J. Brumfitt; A. Cardesín; D. Coia; Marc Costa; Ramos Fernández; D. J. Frew; J. Gallegos; J. J. García Beteta; Bernhard Geiger; D. Heather; T. Lim; Patricia Martín; C. Muñoz Crego; M. Muñoz Fernandez; A. Villacorta


Biophysical Journal | 2018

Voltage-Dependent Conformational Changes of KV1.3 Potassium Channels are an Essential Element for KV1.3-induced cell proliferation

M. Teresa Pérez-García; Pilar Cidad; Esperanza Alonso; Pablo Fernández-Velasco; Miguel A. de la Fuente; José R. López-López


Biophysical Journal | 2016

Voltage-Dependent Accessibility of Phosphorylation Sites at the Carboxy-Terminal Domain of Kv1.3 Channels Determines Kv1.3-Induced Cell Proliferation

Teresa Pérez-García; Pilar Cidad; Laura Jiménez-Pérez; I Alvarez-Miguel; Alba Santos-Hipólito; Esperanza Alonso; Miguel A. de la Fuente; José R. López-López


Proceedings of The Physiological Society | 2014

Molecular mechanisms involved in the effects of Kv1.3 and Kv1.5 channels on cell proliferation

Laura Jiménez-Pérez; Pilar Cidad; I Alvarez-Miguel; R Torres-Merino; Esperanza Alonso; Ma de la Fuente; Jr L; M Perez-Garcia


Proceedings of The Physiological Society | 2014

Changes in the expression and the function of vascular TRPC3 channels in a mouse model of essential hypertension

I Alvarez-Miguel; Pilar Cidad; Esperanza Alonso; M Perez-Garcia; Jr L


Journal of the American College of Cardiology | 2012

ROLE OF KV1.3 CHANNELS IN INTIMAL HYPERPLASIA

Mercè Roquè. Laura Novensa; Pilar Cidad; Montserrat Batlle; Eduardo Miguel-Velado; Esperanza Alonso; Teresa Pérez-García; José Ramón López; Magda Heras

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Pilar Cidad

University of Valladolid

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M. Teresa Pérez-García

Spanish National Research Council

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Eduardo Miguel-Velado

Spanish National Research Council

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