Esther Pérez-Torrero

Chemical Analysis of Nutritional Content of Prickly Pads (Opuntia ficus indica) at Varied Ages in an Organic Harvest

Opuntia ficus indica, also known as prickly pads, are an important part of the human diet and are also used as forage for livestock. This is an interesting vegetable due the environmental conditions in which it grows and its resistance to climatic extremes; however, little is known about its nutritional properties, especially in the later stages of maturity. The objective of this study was to determine the composition of organic prickly pads (Opuntia ficus indica) at differing stages of growth maturity. Chemical proximate analysis and mineral constituent analysis at different maturation stages were carried out in this investigation. As a result, older prickly pads were found to be an important source of nutritional components such as calcium.

Effects of acute prenatal ethanol exposure on Bergmann glia cells early postnatal development

The effects of acute ethanol exposure during the prenatal phase of Bergmann glia cell (Bgc) generation were evaluated in three postnatal days. Ethanol exposed rats showed Bgc with reduced soma size, decreased number and width of their fibers, and increased fiber length, when compared with control animals. These differences, however, were significant at postnatal day 12. Our results demonstrate that acute, prenatal exposure to ethanol during critical stages of brain development disrupts Bgc early postnatal development.

Effects of perinatal undernourishment on neuronal development of the facial motor nucleus in the rat

The facial nucleus (FN) of the rat is composed of multipolar neurons generated between gestational days G12 and G15. This nucleus is involved in the mechanisms underlying muscle contraction during the sucking reflex. After birth, the neuronal substrate of this reflex is gradually organized to allow the performance of other functions such as gnawing, chewing, swallowing and drinking. Undernourishment is known to produce different degrees of delayed brain development, the greatest of which are similar to the characteristics of the premature syndrome. Neuronal morphological alterations are associated with sucking-reflex deficiencies, which interfere with feeding by the newborn. The current study shows that perinatal undernourishment leads to dendritic arbor hypoplasia and small alterations of soma size in Golgi--Cox impregnated FN neurons of rats. The data suggest that these morphological alterations of FN neurons, may be associated with shifts in the input and integration of signals, and may finally modify the elaboration of motoneuron discharges partly modulating bucolabial muscle contraction during sucking movements and facial expression. Additionally, neonatal nutritional rehabilitation modifies the effects on FN neuronal development, ameliorating the influence of early adverse nutritional conditions.

A Paradigm of Undernourishing and Neonatal Rehabilitation in the Newborn Rat

Abstract Perinatal undernutrition as a deficiency of nutrient availability, affects body and brain developmental processes and promotes recurrent health problems. Thus, altered mother-litter bonds and deficient environmental interactions may interfere with the brain pluripotential capabilities of the newborn. To gather information concerning the mechanisms underlying perinatal undernutrition we designed a paradigm of undernutrition and neonatal rehabilitation in the rat. An underfed group came from pregnant Wistar rats fed with 50% of the diet from G6 to G12 and with 60% from G13 until G21. After birth, pups were daily undernourished during 12 h daily by rotating a pair of lactating well-nourished dams which had one of their nipples subcutaneously ligated. The rehabilitated animals were undernourished pups neonatally fed by a pair of normally lactating dams. Controls received plenty of food during the pre- and neonatal periods. Pups were sacrificed at 12, 20 and 30 days of age. Perinatal underfeeding significantly reduced body and brain weights and neuronal morphometric parameters. Normal neonatal feeding in the newborn ameliorated the damages associated to food deprivation. The current undernourishing paradigm may be helpful to assess brain development alterations, as well as to study the compensatory mechanisms associated to salutary epigenetic influences.

Neonatal Nutritional Rehabilitation of Morphological Features in Facial Motoneurons Altered by Prenatally Food Deprivation in the Rat

Abstract Undernutrition alters facial motoneurons development. Here, we evaluated the plastic characteristics of facial motoneurons in response to neonatal rehabilitation. Prenatally undernourished rat pups derived from pregnant rats fed with 50% of the diet from gestational days G6 to G12, and with 60% of food from G13 to G21 and rehabilitated after birth by a pair of control lactating foster dams. Morphological features of 640 Golgi-Cox impregnated motoneurons from 12, 20 and 30 days old pups were analyzed. Neonatal food rehabilitation increased the number and extension of the dendritic branches (main neuronal reception area) without altering significantly soma measurements (main neuronal firing area), suggesting that food and sensory maternal disponibility after birth ameliorates the damage due to prenatal deprivation. Findings showed that neonatal rehabilitation in addition to intense maternal polysensorial stimuli from the foster mothers partially diminished the morphological alterations associated to prenatal undernutrition, and provide evidence of plastic properties of facial motoneurons to the influence of environmental cues.

Early undernourishment interferes with the maternal aggressive response triggered by an intruder entering the homing cage

Abstract The maternal aggressive response (MAR) against intruders is temporarily expressed during lactation in association with the rearing and protection of offspring to promote their survival and growth in the nest. This normal component of maternal behaviour requires both the hormonal changes occurring at the end of pregnancy and the presence of pups for its establishment. Because early food restriction in the rat results in long-term maternal deficiencies, we analysed in Wistar rats the effects of perinatal undernutrition on the MAR to an intruder at days 1, 4, and 8 postpartum. The data showed that undernourished dams exhibited significant reductions of sniffing frequency on days 4 and 8 and significant increases of biting on day 4 and of lateral attacks on postpartum days 4 and 8. The finding of an altered MAR during the lactating period may be relevant for the survival and long-term behavioural development of the progeny.

Mineral Content and Physicochemical Properties in Female Rats Bone During Growing Stage

The atomic absorption spectrophotometry (AAS) is a technique extensively used for trace and ultra trace analysis of organic and inorganic materials. AAS is a novel method that determines in liquid samples the presence of metals such as: Ca, Fe, Cu, Al, Pb, Zn, and Cd from different sources. The determination of minerals is important in environmental and biological studies as well as in the clinical practice. The determination of mineral content is a key to understand changes in some metabolism that conduct to diseases as result of the increase or decrease of mineral components in diet and consequently, to develop new models in the field of animal and human nutrition. In the case of biological samples including plant leaves, fruits, vegetables, organic vegetables i.e. nopal (Opuntia ficus indica), Rodriguez-Garcia et al., (2007) reported that the major mineral components in this cactus was as follows: Calcium, Magnesium, Sodium, Manganese, Iron, Zinc, Potassium, and minor mineral components were: Lithium, Vanadium, Cobalt, Arsenic, Selenium, Cadmium, Thallium (Hernandez-Urbiola et al., 2010, 2011). AAS has been used also to analyze muscle tissue, blood, urine, hair, bones, among others (Martinez-Flores et al., 2002; Christian, 1972). In most cases complex nature of biological materials requires dry ashing followed by wet digestion with oxidizing acids, i.e. HNO3 and HCl4.