Ethan S. Simon

Enzyme-catalyzed synthesis of carbohydrates

A review about the potential of enzymes as catalysts for the synthesis of carbohydrates: advances in the enzyme-catalysed construction of natural and unnatural monosaccharides; methods for enzyme-catalysed formation of glycosyl bonds by Lenoir and non-Lenoir pathways

D-Lactate dehydrogenase: substrate specificity and use as a catalyst in the synthesis of homochiral 2-hydroxy acids

This note compares the substrate specificity of D-lactate dehydrogenase (D-LDH, EC 1.1.1.28) to that of L-lactate dehydrogenase (L-LDH, EC 1.1.1.27), illustrates three procedures that use D-LDH in synthesis and two methods for recycling NADH, and provides experimental details illustrating the use of D-LDH in organic synthesis.

Synthesis of 3-Deoxy-D-manno-2-octulosonate-8-phosphate (KDO-8-P) fromD-Arabinose: Generation of D-Arabinose-5-Phosphate using Hexokinase☆

Abstract Three enzymes (used in soluble form enclosed in a dialysis membrane) efficiently produce 3-deoxy-D-manno-2-octulosonate-8-phosphate (KDO-8-P, 4) from D-arabinose (Ara, 1) and phosphoenolpyruvate (PEP 3); production of D-Ara-5-P 2from Ara using hexokinase is the key step in the synthesis.

D-lactate dehydrogenase

This note compares the substrate specifity of D-lactate dehydrogenase (D-LDH, EC 1.1.1.28) to that of L-lactate dehydrogenase (L-LDH, EC 1.1.1.27), illustrates three procedures that use D-LDH in synthesis and two methods for recycling NADH, and provides experimental details illustrating the use of D-LDH in organic synthesis.

Generation of cytidine 5'-triphosphate using adenylate kinase

Abstract A membrane-enclosed enzyme reactor containing adenylate kinase (E.C. 2.7.4.3, rabbit muscle) and pyruvate kinase (E.C. 2.7.1.40) converts cytidine 5′-monophosphate (CMP) and phosphoenolpyruvate (PEP) to cytidine 5′-triphosphate (CTP) and pyruvate on a gram scale.