Etsuji Matsunaga

The Proteoglycans in Hypertrophic Scar

Sulfated proteoglycans of hypertrophic scar (6 months old after a burn) were extracted with 6 M hot urea, 0.02 M phosphate buffer, pH 7.0, containing protease inhibitors. Two types of proteoglycans, dermatan sulfate proteoglycans and chondroitinsulfate rich proteoglycans, were recovered. The dermatan sulfate proteoglycans, which are capable of binding to concanavalin A, were composed of Mr=53000 core protein and iduronate rich dermatan sulfate. The chondroitin sulfate rich proteoglycans did not bind to concanavalin A but had the same size core proteins as the dermatan sulfate proteoglycans, based on SDS‐polyacrylamide gel electrophoresis. The tryptic peptide mappings showed that the core proteins from dermatan sulfate have the same structural sequences as those from chondroitin sulfate rich proteoglycans.

Acidic glycosaminoglycans, isolation and structural analysis of a proteodermatan sulfate from dermatosparactic calf skin.

Dermatan sulfate and hyaluronic acid are the major glycosaminoglycan components of dermatosparactic and normal calf skin but the ratio of hyaluronic acid to dermatan sulfate is 50-70% higher in dermatosparactic calf skin than that in normal calf skin. Hyaluronic acid in normal calf skin could be extracted to 93% of the total by NaCl and guanidine hydrochloride, while in dermatosparactic calf skin 57% of hyaluronic acid remained in the insoluble material after NaCl and guanidine hydrochloride extraction. The recovery of proteodermatan sulfate in NaCl extracts was similar in normal and dermatosparactic skin. The amount of proteodermatan sulfate extracted in guanidine hydrochloride form normal calf skin was 2-4 fold higher than that from dermatosparactic calf skin. No significant difference between dermatosparactic and normal calf skin proteodermatan sulfate could be detected in terms of size of the core protein (Mr = 55,000) by sodium dodecyl sulfate polyacrylamide gel electrophoresis, amino acid analysis, immunological cross-reactivity using polyclonal antibodies against either dermatosparactic or normal calf skin core protein, tryptic peptides or size of dermatan sulfate chains (Mr = 17,000-18,000) on gel chromatography.

Delayed Cutaneous Metastasis of Gastric Carcinoma

This paper describes a case of skin cancer resembling carcinoma en cuirasse associated with multiple bone lesions, which were both judged to be due to metastasis of poorly differentiated carcinoma of the stomach, the primary lesion excised ten years previously. Overt bone involvement had been observed for more than two years and a half. Histologic examination of a skin biopsy specimen showed that carcinoma cells had infiltrated into the middle and lower dermis, forming clusters and strands in an abundunce of fibrous stromata. No primary lesion was detected in the remnant stomach at autopsy.

Age-related changes of the dermatan sulfate containing small proteoglycans in bovine tendon.

Sulfated proteoglycans of fetal, newborn and adult bovine tendon are extracted with neutral salt and then with 4 M guanidine HCl in the presence of proteinase inhibitors. Dermatan sulfate containing small proteoglycans are separated from chondroitin sulfate rich proteoglycans by CsCl-gradient centrifugation, by affinity chromatography using concanavalin A and by molecular sieve chromatography. These proteoglycans are comprised of a core protein with an average Mr of about 53,000 on polyacrylamide gel electrophoresis with SDS and of dermatan sulfate side chains with Mr 25,000-38,000 by gel chromatography. The tryptic peptides patterns observed in the small proteoglycans from newborn calf and adult bovine tendon are identical but are distinct from those of embryonic calf tendon. The patterns of the tryptic peptides of the core proteins from embryonic calf tendon are similar to those from the dermatan sulfate proteoglycans of calf skin. These results indicated that genetically independent genes of the core proteins from dermatan sulfate proteoglycans in tendon tissues were expressed by aging.

A case of subacute cutaneous lupus erythematosus with a high titer of RNP antibody.

A 30‐year‐old man developed severe erosive lesions in his oral cavity and transient Raynauds phenomenon followed by discoid lupus erythematosus (DLE)‐like lesions and non‐scarring erythema. He had a high titer of RNP antibody as well. Widespread DLE (w‐DLE), subacute cutaneous lupus erythematosus (SCLE) and mixed connective tissue disease (MCTD) were suspected as his condition. Finally we diagnosed him as having SCLE accompanied with a high titer of RNP antibody.

Statistical studies on basal cell epitherioma at the Medical College of Oita Hospital, Oita prefecture.

我々は, Aggressive BCE (Basal Cell Epitheliolna) を (1) 臨床的に再発例, (2) 組織学的に筋層, 軟骨あるいは骨へ達する例のうちいずれかを満たすものと定義づけし, 大分医大皮膚科における59例のBCEをAggressive BCE (14例) とNon-Aggressive BCE (45例) の2群に分け組織学的に比較検討した。その結果, Aggressive BCEではirregular spiky pattern, micronodular nest, peripheral palisading irregularityが組織学的特徴として認められた。炎症細胞浸潤の程度, 潰瘍形成は二つのグループの間に差を認めなかった。これらの結果にもとづき, 当科ではAggressive BCEに対して通常のBCEに対する切除範囲では不十分と考え, SCCに準じた皮膚広範囲切除を行っている。