Eugênia Maria Amorim Ubiali

Zika virus RNA detection in asymptomatic blood donors during an outbreak in the northeast region of São Paulo State, Brazil, 2016: ZIKA VIRUS IN BRAZILIAN BLOOD DONORS

In 2015, there was a large Zika virus (ZIKV) outbreak in Brazil. The proportion of asymptomatic infections is very high, and it is possible for transfusion‐transmitted ZIKV (TT‐ZIKV) infection to occur. The prevalence of asymptomatic ZIKV infection among Brazilian blood donors during this epidemic outbreak is unknown.

Prevalence of Trypanosoma Cruzi antibodies in blood donors from the Sao Paulo State, Brazil, between 2012 and 2014

INTRODUCTION American tripanosomiasis (Chagas disease), the second most neglected disease in the world, is caused by the protozoan parasite Trypanosoma cruzi. Though natural transmission by insect vectors has been controlled, there is significant risk of T. cruzi transmission by blood transfusion in non-endemic regions, generally due to immigration processes from endemic areas. METHODOLOGY The objective of this study was to evaluate anti-T. cruzi seroprevalence in blood donors from the western part of São Paulo State, Brazil, by serologic and immunofluorescence confirmation tests for the period between 2012 and 2014. Currently, this region is regarded as a non-endemic area for Chagas disease. RESULTS The confirmed overall T. cruzi seroprevalence among blood donors was 0.10%, which can be considered low compared to other Brazilian regions. Nevertheless, the distribution of the anti-T. cruzi antibodies within the examined region was uneven, and some areas of significantly higher prevalence were observed. CONCLUSIONS We could consider two tendencies in the prevalence of T. cruzi: (i) residual older undiagnosed cases from São Paulo State, and (ii) immigration from endemic Brazilian or South American regions. The discordance obtained for T. cruzi prevalence by serologic and immunofluorescence methods demonstrates that more specific routine diagnosis is needed to diminish the cost of the assays and the loss of blood supply once all seropositive blood bags are immediately discarded.

Is there justification for universal leukoreduction

The Associacao Brasileira de Hematologia, Hemoterapia e Terapia Celular (ABHH), the institution that represents the Associacao Medica Brasileira (AMB) in respect to hematology, transfusion therapy and cell therapy in Brazil hereby states: Prospective randomized studies have not demonstrated any positive clinical impact with the universal use of blood components in which the initial number of leukocytes has been reduced (universal leukoreduction). For this reason, universal leukoreduction remains a technically controversial topic. The current consensus is that leukoreduction has defined indications in the prevention of three blood transfusion complications only: (i) non-hemolytic febrile reactions when the patient has had this reaction previously; (ii) platelet refractoriness caused by alloimmunization against leukocyte antigens and (iii) the transmission of cytomegalovirus

Evaluation of human T-lymphotropic virus prevalence/co-infection rates for a four-year period in a non-metropolitan blood center in Southeast Brazil

INTRODUCTION Human T-lymphotropic virus types 1/2 (HTLV-1/2) are distributed worldwide and are endemic in specific regions. METHODS Serological evaluation of the HTLV-1/2 prevalence and co-infection rate [human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV), Chagas disease, and syphilis)] for 2011-2014 was performed with volunteer blood donors from the western part of São Paulo State. RESULTS Serrana and Araçatuba had higher HTLV seroprevalence rates (0.1%); while Franca, Olimpia, and Bebedouro had lower seroprevalences (0.04%). Co-infection (HBV and syphilis) was present in 12.3% of HTLV-infected blood donors. CONCLUSIONS Our findings provide data for the prevalence of HTLV in Brazil and demonstrate the importance of regional and global hemovigilance.

Antimicrobial activity of two techniques for arm skin disinfection of blood donors in Brazil

Objective: Evaluation of the antimicrobial effect of skin disinfection techniques is essential to avoid the transmission of infectious agents during blood transfusion. The aim of this study was to examine the effectiveness of two methods of arm skin disinfection used in blood donors at a Hemotherapy Center in Brazil that represents an important centre for distributing haemocomponents to many cities in the country.

Guidelines on Beta-thalassemia major - regular blood transfusion therapy: Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular: project guidelines: Associação Médica Brasileira - 2016

he guidelines project is a joint initiative of the Associação édica Brasileira and the Conselho Federal de Medicina. It aims o bring together information in medicine to standardize onduct in order to help decision-making during treatment. he data contained in this article were prepared by and re recommended by the Associação Brasileira de Hematologia, emoterapia e Terapia Celular (ABHH). Even so, all possible conucts should be evaluated by the physician responsible for

Custo médio do Módulo de Coleta de sangue total pelo método ABC

Os procedimentos em hemoterapia sao complexos e caros. Exigem processos controlados e validados, equipamentos calibrados e monitorados e insumos qualificados, validados e inspecionados antes e durante o uso. Isto acarreta, alem dos gastos diretos, gastos indiretos especificamente relacionados a garantia da qualidade e da seguranca transfusionais, alem dos gastos indiretos usuais de qualquer produto ou servico. Procurando avaliar com maior aproximacao estes custos e buscando evitar as distorcoes das apropriacoes de custos por rateios, o presente estudo utilizou o sistema de Custeio Baseado em Atividades - ABC, para apurar o custo medio do Modulo de Coleta de sangue total no Hemocentro de Ribeirao Preto - SP, unidade sede, no primeiro semestre de 2006. O maior impacto no custo medio apurado se deveu aos custos monetarios diretos, entretanto os custos indiretos nao foram despreziveis. O custo medio obtido para desempenho das atividades que compoem o Modulo de Coleta foi de R

Guidelines on transfusion of red blood cells: Prognosis of patients who decline blood transfusions

35,20, que e 68,75% superior ao valor pago na tabela SIA/SUS para este modulo. A metodologia desenvolvida pode ser aplicada aos outros procedimentos dos servicos de hemoterapia, permitindo a avaliacao dos custos de seus processos, evitando desperdicios, aprimorando o seu funcionamento e gerando evidencias objetivas que demonstrem os custos reais da hemoterapia de qualidade para as instâncias financiadoras publicas e privadas.

Effect of red blood cell preservation by droplet freezing with non-permeable cryoprotective agents in blood group antigen reactivity

Faculdade de Ciências Médicas da Santa Casa de São Paulo (FCMSCSP), São Paulo, SP, Brazil Hemocentro de Ribeirão Preto, Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo HCFMRP-USP), Ribeirão Preto, SP, Brazil Hemocentro da Universidade Estadual de Campinas (Unicamp), Campinas, SP, Brazil Fundação Pró-Sangue, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (HCFMUSP), São Paulo, SP, Brazil Associação Médica Brasileira (AMB), São Paulo, SP, Brazil

Manual de medicina transfusional

In the last few decades, various red blood cell (RBC) freezing techniques have been developed and improved to enable the preservation of erythrocytes for future use in pre‐transfusion tests in reference immunohaematology laboratories. However, not all these techniques have been sufficiently evaluated for the preservation of blood group antigens.