Eugenio Cazzato

Quality, yield and nitrogen fixation of faba bean seeds as affected by sulphur fertilization

Abstract Faba bean (Vicia faba L.) is an important source of plant protein for humans and animals; however, nutritional value of seeds is notoriously deficient in sulphur (S)-containing amino acids. In this article, the effect of S fertilization on faba beans capability of N2 fixation, grain yield and chemical characteristics in terms of protein fractions, fatty acids and minerals composition is reported. A randomized, complete block design with three replicates was used, and three S applications (0, 30 and 60 kg ha−1, respectively) for faba bean were performed. The S fertilization was split into two applications: 50% before sowing and 50% in the beginning of March as K2SO4. At the same time, both the legume and oat crops were fertilized uniformly with 10 kg N ha−1 as 15N NH4 15NO3 (10% 15N atomic excess) in solution form. In a Mediterranean climate under optimal spring rainfall situations, faba bean produced high yield of grain and protein. Sulphur application resulted in an increase in overall plant yield and N2 fixation. In addition, S fertilization enhanced the protein quality, increasing its degradable fraction. Fertilizing faba bean with 30 kg ha−1 of S resulted in a more appropriate dose in order to obtain a quantitative and qualitative crop improvement. From our findings, it can be concluded that S fertilization to faba bean should be recommended to soils with suboptimal S levels to obtain maximum seed and protein yields.

Suitability of partly destoned exhausted olive cake as by-product feed ingredient for lamb production

The effect of diets with different levels of partly destoned exhausted olive cake (PDEOC) on growth performance and carcass traits of Gentile di Puglia breed lambs was studied. Sixty lambs (16.5 ± 0.5 kg) at weaning were randomly allocated to 3 isocaloric and isonitrogenous diets for 50 d. Pelleted total mixed rations (TMR) were formulated to provide olive by-product at 3 different levels: 1) a control diet without olive by-product (PDEOC-0), 2) an experimental corn-based diet containing 10% by-product (PDEOC-10) replacing part of the oat hay and sunflower meal, and 3) an experimental corn-based diet containing 20% PDEOC (PDEOC-20) replacing part of the oat hay and soybean meal. To evaluate in vivo digestibility of the diets, adult rams (n = 3) were placed in metabolic cages, their individual feces and urine were collected, and differences were observed for DM and fiber fractions. Results from the growth trial of the lambs showed that performance was influenced by olive by-product inclusion in diet (P < 0.05). At the end of the feeding period, lambs were slaughtered, and none of the variables studied were influenced by dietary treatment except for cold carcass dressing (P = 0.027) and half-carcass weight (P = 0.019), which were improved in lambs fed the PDEOC-20 diet. As a result, the current study confirms that olive by-product can be used in lamb finishing rations, resulting in a valuable ingredient as replacement for conventional feeds, which could reduce feeding costs because of the lower cost of the olive by-product. Use of olive by-products as animal feed may become economically feasible for producers where the olive oil industries play an important economic role.

Risk assessment of heavy metal and metalloid toxicity through a contaminated vegetable (Cucurbita maxima) from wastewater irrigated area: A case study for a site-specific risk assessment in Jhang, Pakistan

ABSTRACT The present research was conducted in district Jhang, Pakistan, to evaluate the concentration of metals/metalloids in soil and pumpkin (Cucurbita maxima) irrigated with domestic wastewater. Data revealed that the levels of metals and metalloids in soil samples from two different sites were below the safe limits except Cd, whereas, in the vegetable, the concentrations of As, Se, Ni, Mo, Pb, Mn, and Cu were above the safe limits. The levels of 12 metals and metalloids in the soil were ranged between 0.14 to 22.76 mg/kg at site-I and 0.16 to 22.13 mg/kg at site-II. The levels of these metals in the vegetable were found 0.35 to 61.13 mg/kg at site-I and 0.31 to 53.63 mg/kg at site-II. The transfer factor at both sites was highest for As and Co. The pollution load index recorded for Se, Cu, Cd, Mo, Pb, and Co was greater than 1. The daily intake of As, Mn, and Mo was above the oral reference dose, which reflects that the intake of pumpkin is not safe for the inhabitants of the selected sites. The control measures should be taken to phytoextract heavy metals and metalloids from polluted sites so as to reduce the health risks.

Effects of harvest period, nitrogen fertilization and mycorrhizal fungus inoculation on triticale (×Triticosecale Wittmack) forage yield and quality

The practice of grazing winter cereals before heading and subsequently harvesting the forage is common in some countries. Triticale (× Triticosecale Wittmack) is particularly interesting due to its nutritional characteristics, and forage yield and quality are strongly influenced by agronomic techniques. The effect of two modes of utilization (cut at heading stage and cut during winter grazing simulation with regrowth cut at heading stage), three nitrogen (N) fertilization levels [0 and 100 kg ha −1 split in two phases; and 100 kg ha −1 before sowing, using a fertilizer with 3,4-dimethylpyrazole phosphate (DMPP) as a nitrification inhibitor (NI)] and mycorrhizal fungus inoculation (mixed inoculation of Glomus intraradices and Glomus moseae ) on forage yield and quality, protein fractions and in vitro digestibility of triticale was determined in southern Italy. Mode of utilization and fertilization affected significantly forage yield and quality, while mycorrhizal fungus inoculation influenced positively some quality parameters. Cutting at heading stage of triticale led to high dry matter (DM) production compared to the winter cut simulating grazing, but no differences in protein yield (PY). N fertilization increased total DM and PYs, but no differences were reported among the application modalities of N fertilizers. Triticale protein fractions and protein and fiber digestibility were improved by N fertilization. Mycorrhizal fungus inoculation positively influenced forage quality at heading stage, which resulted in more digestible fiber and protein. As a result, when triticale was grown under Mediterranean conditions, mode of utilization and N fertilization resulted in greater forage yield and quality, while mycorrhizal fungus inoculation influenced positively forage nutritional quality parameters.

Effects of harvest date, wilting and inoculation on yield and forage quality of ensiling safflower (Carthamus tinctorius L.) biomass

BACKGROUND Safflower (Carthamus tinctorius L.), usually grown as a source of oil crop, can be used as fodder either for hay or ensiling purposes, particularly in semi-arid regions. RESULTS A 2-year trial was conducted in southern Italy to evaluate the production and forage quality of safflower biomass cv. Centennial, harvested at three different stages: 1, at complete appearance of primary buds (PB); 2, at complete appearance of secondary and tertiary buds (STB); and 3, at 25% of flowering stage (FS). For each stage of growth, 50% of the biomass was ensiled in 4 L glass jars without and with inoculation (Lactobacillus plantarum, LAB), and the other 50% was field wilted for 24 h before ensiling. Dry matter (DM) content and yield (DMY), pH, buffering capacity (BC) and water soluble carbohydrates (WSC) were determined on fresh forage. On safflower silages were also evaluated ammonia-N, crude protein (CP), fibre fractions, fat, lactic and acetic acids, Ca and P, and gas losses. DMY ranged from 4.5 t ha(-1) (PB harvesting) to 11.6 t ha(-1) (FS harvesting). DM content varied from 129 g kg(-1) (PB not wilted) to 630 g kg(-1) (FS wilted). The WSC in forage before ensiling with not wilting ranged from 128 (PB stage) to 105 and 100 g kg(-1) DM at STB and FS stages, respectively. The wilted safflower forage showed a lower WSC compared to wilted forage. The high sugar substrate allowed lactic acid fermentation and a good conservation quality in all the harvesting stages. Silages quality was strongly influenced by the treatment performed. Wilting practice increased DM, pH and NDF contents but reduced lactic acid, acetic acid and NH(3) -N values. Inoculation reduced DM, pH and NDF contents, but increased lactic and acetic acids, CP and ash. CONCLUSION As result, wilting the forage for 1 day was very effective in the early harvesting stage because this practice significantly increased DM, reducing on the same time the intensive fermentation and proteolysis processes of silage. When harvesting is performed at the beginning of the flowering stage wilting is not necessary.

Potential Contribution of Retama raetam (Forssk.) Webb & Berthel as a Forage Shrub in Sinai, Egypt

The shortage of forage in arid areas is an important concern and it leads us to explore alternative options as nonconventional feed resources. Among potential forage species, samples of Retama raetam (R. raetam) were collected from six different locations representing four habitat types in two diverse phytogeographical regions in Egypt during the summer and winter seasons. Soil characteristics, growth performance, and nutritional traits of R. raetam were evaluated to assess the potential value of this legume as an alternative forage source in the Mediterranean ecosystem. Data showed that soil texture across the study sites had sandy, loamy, and clayey textures, whereas pH ranged from neutral to alkaline and organic carbon was low at all sites. Growth and production of R. raetam were significantly affected by seasonal variation in rainfall and, based on our study results, this effect was more important than specific site property variations such as soil texture and organic matter contents. Moreover, data indicate that R. raetam had wide ecological amplitude and growth performance as a function of site and season. Nutritional value of R. raetam was high enough to meet the nutrient requirements of several different grazing animals. Comparing the R. raetam nutritional value with those of the other wild plants, it can be concluded that R. raetam has strong potential as forage crop with valuable nutritional quality for browsing animals. Moreover, R. raetam may represent an alternative feedstuff to the conventional forage and a promising substitute fodder in Mediterranean ecosystem.

Influence of different soil management practices on ground-flora vegetation in an almond orchard

This paper reports a survey on the weed flora and seed bank in an almond orchard sited in Apulia region (Southern Italy), where the following soil management practices have been compared for over 30 yrs: no-tillage, keeping the soil totally weed-free throughout the year by using pre-emergence herbicides to prevent plant emergence or post-emergence herbicides in case of weeds already emerged; no-tillage, with post-emergence herbicides; no-tillage, with mowing of natural weed flora in spring; cover cropping, with faba bean sown in November and green manured in springtime; conventional soil tillage. The different management techniques influenced significantly the weed flora in experimental plots, both in terms of quantity and quality. The seed bank was clearly impoverished after the long-term applications of pre-emergence herbicides, both in terms of richness and of diversity. During the fall period, the plots of conventional tillage or pre-emergence herbicides had less natural ground-flora than the others. During springtime, prior to the sward control practices, the plots treated by foliar herbicides or mowing had the highest total weed cover. We conclude that post-emergence weed control by mowing or using chemical herbicides or the green manure of the cover crop may be proposed to reduce impact to the soil and to promote the growth of abundant and sufficiently diversified and balanced flora. If appropriately managed, this flora can provide potential ecological services, without competing with the orchard, as suggested by the literature. During the autumn, natural flora can uptake soil nitrogen thus preventing leaching in the rainy season. In springtime, after the sward has been destroyed, natural flora can supply a substantial amount of biomass to the soil. Indicator species analysis was also used to find the species characterizing each treatment and some of their combinations. Weeds belonging to the Poaceae botanical family were significantly associated with post-emergence herbicides and mowing treatments. These species produce a substantial amount of biomass and have bunched roots; consequently, they supply beneficial effects by improving porosity and structure of the soil and reducing erosion hazard.

Forage yield and quality of emmer (Triticum dicoccum Schübler) and spelt (Triticum spelta L.) as affected by harvest period and nitrogen fertilization

Emmer (Triticum dicoccum Schübler) and spelt (Triticum spelta L.) are two ancient cereal crops which have been traditionally grown in the semiarid areas of the Mediterranean basin. The renewed interest in these species has its origin in favorable quality parameters of grain. Unfortunately, there is a lack of information on hulled wheat characteristics when a whole plant is used as forage. Therefore, a field trial was carried out in southern Italy during 2010–2011 to evaluate the effect of N applications (0, 40, and 80 kg ha−1) and harvest period (cut at boots just swollen and cut at early dough) on forage yield and quality of two emmer varieties (“Padre Pio” and “Giovanni Paolo”) and one of spelt (“Altamura”) on their biomass yield and nutritional traits as well as carbohydrates fractionation. Data indicated that the harvest stage and genotype affected forage yield and quality significantly, while N fertilization has influenced some qualitative parameters. Old wheat cultivars tested had considerable yield and forage quality, and they seem to be adapted to marginal lands in the absence of N fertilization. Therefore, from our findings we can conclude that the emmer could be recommended for hay production harvested at booting stage given its valuable nutritional characteristics, whereas spelt results to be more adaptable for silage production when harvested at the early dough stage.

Quantitative and Qualitative Traits of Natural Ecotypes of Perennial Grasses (Dactylis glomerata L., Festuca arundinacea Schreb., Phalaris tuberosa L., Brachypodium rupestre (Host) R. et S.) Collected in Southern Italy

In 1999, natural populations of Dactylis glomerata L., Festuca arundinacea Schreb., Phalaris tuberosa L. and the macrotherm species Brachypodium rupestre (Host) R. et S., were collected as seed in Southern Italy (Apulia, Basilicata and Campania regions) to evaluate the potential as forage of native germplasm under dry conditions, using available commercial cultivars as controls. The persistence of accessions, biometric, phenologic, productive and qualitative parameters of forage have been studied for a 3-year period (2000–2002) in a typical Mediterranean environment. D. glomerata is widely present in the studied area with two subspecies (subsp. glomerata L., subsp. hispanica (Roth) Nyman). The research pointed out significant differences in plant size, earliness and single plant dry matter (DM) production. All the natural ecotypes belonging to this species showed higher persistence than the control cultivars. Some natural ecotypes appeared to have potential for improving DM yield (subsp. glomerata ecotypes) and reducing neutral detergent fibre (NDF) content (subsp. hispanica ecotypes). Natural ecotypes belonging to F. arundinacea, P. tuberosa, and B. rupestre are sporadically present in the studied area. Among them, two ecotypes of P. tuberosa, showing higher winter growth and earliness compared to cv. ‘Holdfast’, seem to be interesting for a future breeding programme.

Effects of Conservative Tillage and Nitrogen Management on Weed Seed Bank after a Seven-Year Durum Wheat—Faba Bean Rotation

Conservative agriculture includes a range of management strategies with low energy inputs such as no-tillage, minimum tillage, and low application of fertilizers. Weed flora in arable fields is strictly affected by agronomic practices such as tillage and fertilization management. This study was conducted seven years after the beginning of a long-term—durum wheat–faba bean—rotation. It analyzes the combined effects on the soil seed bank of three different tillage systems (conservative, reduced, and conventional tillage) and two levels of nitrogen fertilization. The effects were investigated both using stepwise discriminant analysis and analysis of variance in order to find statistical differences among main factors and their interactions. The seed bank of Conyza canadensis, Papaver rhoeas, Solanum nigrum, Fallopia convolvulus, and Fumaria officinalis was higher in conservative or reduced tillage plots. The magnitude of the response to nitrogen supply varied among weed species. Conyza canadensis seemed to be favored by low nitrogen supply, whereas Sinapis arvensis by higher doses of nitrogen. Anagallis arvensis showed the lowest seed bank in conventionally tilled plots, without distinction of nitrogen supply. The results suggest that different tillage systems and, to a lesser extent, different nitrogen supply, produce changes in the seed bank size and composition, along the soil profile.