Eun Jung Choy

CONDITION, REPRODUCTIVE ACTIVITY, AND GROSS BIOCHEMICAL COMPOSITION OF THE MANILA CLAM, TAPES PHILIPPINARUM IN NATURAL AND NEWLY CREATED SANDY HABITATS OF THE SOUTHERN COAST OF KOREA

Abstract Sand was added to the mudflat in a small bay on the southern coast of Korea in an attempt to create a new habitat for the Manila clam (Tapes philippinarum) in the muddy intertidal zone. To evaluate whether the newly created sandy habitat was functionally similar to natural ones, seasonal variations in condition, reproductive activity, and biochemical composition of clams in created and natural conditions were compared from May 2000 to October 2001. Clams reared in the newly created and natural habitats had similar patterns and levels with respect to condition and tissue dry weight. Standardized animal condition and tissue dry weight of clams peaked in spring, when protein and carbohydrate reserves were at maximum levels, and declined progressively throughout the summer-autumn period to October, as a result of continuous spawning. Condition and tissue weight were quickly restored during the winter-spring period, concurrently with accumulation of protein and carbohydrate reserves. Similar biochemical compositions and reproductive cycles for the clam stocks in the two habitats are likely to be related to their similar environmental conditions, in particular food availability. Comparison of the isotopic signatures of T. philippinarum tissues and potential food resources suggested that food availability in the study area was mostly dependent on resuspension of microphytobenthos, along with seasonal dynamics of phytoplankton. These observations clearly show that newly created sandy habitats may provide habitat functions that enable Manila clams to have similar biological cycles to those in natural habitats.

Linking Intertidal and Subtidal Food Webs: Consumer-Mediated Transport of Intertidal Benthic Microalgal Carbon.

We examined stable carbon and nitrogen isotope ratios for a large variety of consumers in intertidal and subtidal habitats, and their potential primary food sources [i.e., microphytobenthos (MPB), phytoplankton, and Phragmites australis] in a coastal bay system, Yeoja Bay of Korea, to test the hypothesis that the transfer of intertidal MPB-derived organic carbon to the subtidal food web can be mediated by motile consumers. Compared to a narrow δ13C range (−18 to −16‰) of offshore consumers, a broad δ13C range (−18 to −12‰) of both intertidal and subtidal consumers indicated that 13C-enriched sources of organic matter are an important trophic source to coastal consumers. In the intertidal areas, δ13C of most consumers overlapped with or was 13C-enriched relative to MPB. Despite the scarcity of MPB in the subtidal, highly motile consumers in subtidal habitat had nearly identical δ13C range with many intertidal foragers (including crustaceans and fish), overlapping with the range of MPB. In contrast, δ13C values of many sedentary benthic invertebrates in the subtidal areas were similar to those of offshore consumers and more 13C-depleted than motile foragers, indicating high dependence on phytoplankton-derived carbon. The isotopic mixing model calculation confirms that the majority of motile consumers and also some of subtidal sedentary ones depend on intertidal MPB for more than a half of their tissue carbon. Finally, although further quantitative estimates are needed, these results suggest that direct foraging by motile consumers on intertidal areas, and thereby biological transport of MPB-derived organic carbon to the subtidal areas, may provide important trophic connection between intertidal production and the nearshore shallow subtidal food webs.

Stable isotope analysis of a newly established macrofaunal food web 1.5 years after the Hebei Spirit oil spill

We examined trophic relationships in a newly established community 1.5 years after the Hebei Spirit oil spill on the west coast of Korea. Carbon and nitrogen stable isotope ratios in consumers and their potential food sources were compared between the oil-spill site and reference site, located 13.5 km from the oil-spill spot. The isotopic mixing model and a novel circular statistics rejected the influx of petrogenic carbon into the community and identified spatial consistencies such as the high contributions of microphytobenthos, food-chain length, and the isotopic niche of each feeding guild between sites. We suggested that high level of trophic plasticity and the prevalence of omnivory of consumers may promote the robustness of food web against the oil contamination. Furthermore, we highlighted the need of holistic approaches including different functional groups to quantify changes in the food web structure and assess the influence of different perturbations including oil spill.

Effects of ocean acidification driven by elevated CO2 on larval shell growth and abnormal rates of the venerid clam, Mactra veneriformis

The venerid clam (Mactra veneriformis Reeve 1854) is one of the main cultured bivalve species in intertidal and shallow subtidal ecosystems along the west coast of Korea. To understand the effects of ocean acidification on the early life stages of Korean clams, we investigated shell growth and abnormality rates and types in the D-shaped, umbonate veliger, and pediveliger stages of the venerid clam M. veneriformis during exposure to elevated seawater pCO2. In particular, we examined abnormal types of larval shell morphology categorized as shell deformations, shell distortions, and shell fissures. Specimens were incubated in seawater equilibrated with bubbled CO2-enriched air at (400±25)×10-6 (ambient control), (800±25)×10-6 (high pCO2), or (1 200±28)×10-6 (extremely high pCO2), the atmospheric CO2 concentrations predicted for the years 2014, 2084, and 2154 (70-year intervals; two human generations), respectively, in the Representative Concentration Pathway (RCP) 8.5 scenario. The mean shell lengths of larvae were significantly decreased in the high and extremely high pCO2 groups compared with the ambient control groups. Furthermore, under high and extremely high pCO2 conditions, the cultures exhibited significantly increased abundances of abnormal larvae and increased severity of abnormalities compared with the ambient control. In the umbonate veliger stage of the experimental larvae, the most common abnormalities were shell deformations, distortions, and fissures; on the other hand, convex hinges and mantle protuberances were absent. These results suggest that elevated CO2 exerts an additional burden on the health of M. veneriformis larvae by impairing early development.