Eun Kyung Park

The Enzymatic Regulatory Effects of Laninaria japonica Fucoidan Extract in Hepatotoxicity

The purpose of this study was to investigated the effects of Laminaria japonica fucoidan extract (LJFE) through the enzymatic regulation against the hepatotoxicity-inducing carbon tetrachloride (CCl 4 ) in LJFE and CCl 4 -treated rats. LJFE of 100 mg/kg concentration was intraperitoneally administered into rats at dose of 1.5ml/kg for 14 days. On the day 15, 3.3ml/kg of CCl 4 dissolved in olive oil (1:1) was injected 12 hours before anesthetization. We examined the levels of glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT) in serum of rats, superoxide dismutase(SOD) in mitochondrial fraction, and catalase(CAT), glutathione peroxidase(GPx) in liver homogenate. CCl 4 -treatment markedly increased the levels of GOT and GPT, and significantly decreased those of SOD, CAT and GPx. But LJFE pretreatment decreased the levels of GOT and GPT, by 40% and 64%, respectively and increased those of SOD, CAT and GPx, by 114%, 36.1% and 55.9%, respectively. These results showed the LJFE had the enzymatic regulatory effects against the hepatotoxicity-inducing CCl 4 in the preventive way.