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Dive into the research topics where Eva Sevcsik is active.

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Featured researches published by Eva Sevcsik.


Biophysical Journal | 2008

Interaction of LL-37 with Model Membrane Systems of Different Complexity: Influence of the Lipid Matrix ☆

Eva Sevcsik; Georg Pabst; Walter Richter; Sabine Danner; Heinz Amenitsch; Karl Lohner

As the main difference between bacterial and mammalian cell membranes is their net charge, the focal point of consideration in many model membrane experiments with antimicrobial peptides is lipid headgroup charge. We studied the interaction of the human multifunctional peptide LL-37 with single phospholipid monolayers, bilayers, and bilayers composed of binary mixtures of the four phospholipid species predominantly used in model membrane experiments (phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylserine). We found that 1), the effects on single lipid monolayers are not comparable to those on the corresponding bilayers; 2), there are four different effects of LL-37 on bilayers of the four lipids; 3), the preference of LL-37 for the specific lipids is roughly inversely related to chain packing density; and 4), in the binary lipid mixtures, one lipid-and not necessarily the charged one--generally governs the mode of lipid/peptide interaction. Thus, our results show that lipid net charge is not the decisive factor determining the membrane-perturbing mechanism of LL-37, but only one of several parameters, among them packing density, the ability to form intermolecular H-bonds, and lipid molecular shape, which emphasizes how profoundly the choice of the model system can influence the outcome of a study of lipid/peptide interaction.


Nature Communications | 2015

GPI-anchored proteins do not reside in ordered domains in the live cell plasma membrane

Eva Sevcsik; Mario Brameshuber; Martin Fölser; Julian Weghuber; Alf Honigmann; Gerhard J. Schütz

The organization of proteins and lipids in the plasma membrane has been subject of a long-lasting debate. Membrane rafts of higher lipid chain order were proposed to mediate protein interactions, but have thus far not been directly observed. Here, we use protein micropatterning combined with single-molecule tracking to put current models to the test: we rearranged lipid-anchored raft proteins (glycosylphosphatidylinositol(GPI)-anchored mGFP) directly in the live cell plasma membrane and measured the effect on the local membrane environment. Intriguingly, this treatment does neither nucleate the formation of an ordered membrane phase, nor result in any enrichment of nanoscopic ordered domains within the micropatterned regions. In contrast, we find that immobilized mGFP-GPIs behave as inert obstacles to the diffusion of other membrane constituents without influencing their membrane environment over distances beyond their physical size. Our results indicate that phase partitioning is not a fundamental element of protein organization in the plasma membrane.


BioEssays | 2016

With or without rafts? Alternative views on cell membranes

Eva Sevcsik; Gerhard J. Schütz

The fundamental mechanisms of protein and lipid organization at the plasma membrane have continued to engage researchers for decades. Among proposed models, one idea has been particularly successful which assumes that sterol‐dependent nanoscopic phases of different lipid chain order compartmentalize proteins, thereby modulating protein functionality. This model of membrane rafts has sustainably sparked the fields of membrane biophysics and biology, and shifted membrane lipids into the spotlight of research; by now, rafts have become an integral part of our terminology to describe a variety of cell biological processes. But is the evidence clear enough to continue supporting a theoretical concept which has resisted direct proof by observation for nearly twenty years? In this essay, we revisit findings that gave rise to and substantiated the raft hypothesis, discuss its impact on recent studies, and present alternative mechanisms to account for plasma membrane heterogeneity.


Advances on Planar Lipid Bilayers and Liposomes | 2008

Chapter Five Liposome-Based Biomembrane Mimetic Systems: Implications for Lipid–Peptide Interactions

Karl Lohner; Eva Sevcsik; Georg Pabst

Abstract The fundamental structural unit of biological membranes is mostly a highly dynamic, liquid-crystalline phospholipid bilayer that acts as a permeability barrier. The concept of a characteristic lipid composition for a given cell membrane is well-accepted and is of considerable interest, when studying the molecular mechanism(s) of membrane damage by membrane-active agents such as toxins or antimicrobial peptides. Despite the wealth of information and experimental data we still do not fully understand at a molecular level how these peptides disrupt the barrier function of cell membranes. Therefore, lipid model membranes mimicking the more complex biological membranes have attracted scientists from various fields. Structural and thermodynamic characterization of these biomembrane mimetic systems such as liposomes is a prerequisite for the understanding of lipid–peptide interactions. The focus of this contribution will be on how X-ray scattering techniques contribute to the characterization of liposomes and in turn to the elucidation of the mechanisms of peptide–membrane interaction. First, we summarize the current models for the mode of action of antimicrobial peptides as well as general aspects of model membranes followed by a detailed description of X-ray scattering in combination with a global data analysis. The applicability of this new approach is exemplary shown on selected model membrane and lipid–peptide systems demonstrating a tight coupling between the peptide properties and those of the lipid bilayer.


Cytotechnology | 2007

Serum-free transfection of CHO-cells with tailor-made unilamellar vesicles.

Hannes Reisinger; Eva Sevcsik; Karola Vorauer-Uhl; Karl Lohner; Hermann Katinger; Renate Kunert

At present, a number of transfection techniques are available to introduce foreign DNA into cells, but still minimal intrusion or interference with normal cell physiology, low toxicity, reproducibility, cost efficiency and successful creation of stable transfectants are highly desirable properties for improved transfection techniques.For all previous transfection experiments done in our labs, using serum-free cultivated host cell lines, an efficiency value of ∼0.1% for selection of stable cell lines has not been exceeded, consequently we developed and improved a transfection system based on defined liposomes, so-called large unilamellar vesicles, consisting of different lipid compositions to facilitate clone selection and increase the probability for creation of recombinant high-production clones. DNA and DOTAP/DOPE or CHEMS/DOPE interact by electrostatic means forming so-called lipoplexes (Even-Chen and Barenholz 2000) and the lipofection efficiency of those lipoplexes has been determined via confocal microscopy.In addition, the expression of the EGFP was determined by FACS to investigate transient as well as stable transfection and the transfection efficiency of a selection of different commercially available transfection reagents and kits has been compared to our tailor-made liposomes.


Nature Immunology | 2018

Monomeric TCRs drive T cell antigen recognition

Mario Brameshuber; Florian Kellner; Benedikt K. Rossboth; Haisen Ta; Kevin Alge; Eva Sevcsik; Janett Göhring; Markus Axmann; Florian Baumgart; Nicholas R. J. Gascoigne; Simon J. Davis; Hannes Stockinger; Gerhard J. Schütz; Johannes B. Huppa

T cell antigen recognition requires T cell antigen receptors (TCRs) engaging MHC-embedded antigenic peptides (pMHCs) within the contact region of a T cell with its conjugated antigen-presenting cell. Despite micromolar TCR:pMHC affinities, T cells respond to even a single antigenic pMHC, and higher-order TCRs have been postulated to maintain high antigen sensitivity and trigger signaling. We interrogated the stoichiometry of TCRs and their associated CD3 subunits on the surface of living T cells through single-molecule brightness and single-molecule coincidence analysis, photon-antibunching-based fluorescence correlation spectroscopy and Förster resonance energy transfer measurements. We found exclusively monomeric TCR–CD3 complexes driving the recognition of antigenic pMHCs, which underscores the exceptional capacity of single TCR–CD3 complexes to elicit robust intracellular signaling.Higher-order TCRs have been postulated to maintain high antigen sensitivity and trigger signaling. Huppa and colleagues use various investigative techniques and find exclusively monomeric TCR–CD3 complexes that drive the recognition of antigenic pMHC.


Biophysical Journal | 2016

Oxidized Phospholipids Inhibit the Formation of Cholesterol-Dependent Plasma Membrane Nanoplatforms

Mario Brameshuber; Eva Sevcsik; Benedikt K. Rossboth; Christina Manner; Hans Peter Deigner; Begüm Peksel; Mária Péter; Zsolt Török; Albin Hermetter; Gerhard J. Schütz

We previously developed a single-molecule microscopy method termed TOCCSL (thinning out clusters while conserving stoichiometry of labeling), which allows for direct imaging of stable nanoscopic platforms with raft-like properties diffusing in the plasma membrane. As a consensus raft marker, we chose monomeric GFP linked via a glycosylphosphatidylinositol (GPI) anchor to the cell membrane (mGFP-GPI). With this probe, we previously observed cholesterol-dependent homo-association to nanoplatforms diffusing in the plasma membrane of live CHO cells. Here, we report the release of this homo-association upon addition of 1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphocholine (POVPC) or 1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphocholine, two oxidized phospholipids (oxPLs) that are typically present in oxidatively modified low-density lipoprotein. We found a dose-response relationship for mGFP-GPI nanoplatform disintegration upon addition of POVPC, correlating with the signal of the apoptosis marker Annexin V-Cy3. Similar concentrations of lysolipid showed no effect, indicating that the observed phenomena were not linked to properties of the lipid bilayer itself. Inhibition of acid sphingomyelinase by NB-19 before addition of POVPC completely abolished nanoplatform disintegration by oxPLs. In conclusion, we were able to determine how oxidized lipid species disrupt mGFP-GPI nanoplatforms in the plasma membrane. Our results favor an indirect mechanism involving acid sphingomyelinase activity rather than a direct interaction of oxPLs with nanoplatform constituents.


Journal of Physics D | 2016

Monte Carlo simulations of protein micropatterning in biomembranes: effects of immobile sticky obstacles

Andreas Arnold; Eva Sevcsik; Gerhard J. Schütz

Single molecule trajectories of lipids and proteins can yield valuable information about the nanoscopic organization of the plasma membrane itself. The interpretation of such trajectories, however, is complicated, as the mobility of molecules can be affected by the presence of immobile obstacles, and the transient binding of the tracers to these obstacles. We have previously developed a micropatterning approach that allows for immobilizing a plasma membrane protein and probing the diffusional behavior of a putative interaction partner in living cells. Here, we provide guidelines on how this micropatterning approach can be extended to quantify interaction parameters between plasma membrane constituents in their natural environment. We simulated a patterned membrane system and evaluated the effect of different surface densities of patterned immobile obstacles on the relative mobility as well as the surface density of diffusing tracers. In the case of inert obstacles, the size of the obstacle can be assessed from its surface density at the percolation threshold, which in turn can be extracted from the diffusion behavior of the tracer. For sticky obstacles, two-dimensional dissociation constants can be determined from the tracer diffusion or surface density.


Journal of Liposome Research | 2009

Topological transformation of liposomes by a membrane-affecting domain of recombinant human erythropoietin

Stefanie Strobach; Renate Kunert; Johannes Stadlmann; Paul Messner; Eva Sevcsik; Gabriele Lhota; Hermann Katinger; Karola Vorauer-Uhl

Recombinant human erythropoietin (rh-Epo) is well accepted as a hematopoietic drug, but many other pleiotropic properties are currently under investigation. Rh-Epo-induced receptor-mediated signal transductions are accompanied with membrane dynamic processes, which facilitate the activation of individual pathways. However, its direct effect on membrane dynamics is still unknown. In the present study, we have proven the capability of rh-Epo to associate to and transform artificial lipid membranes. Association studies using neutral, negatively, and positively charged liposomes with the native as well as modified rh-Epo were performed and analyzed by transmission electron microscopy and differential scanning calorimetry. By these studies, we demonstrated that rh-Epo has the capability to transform negatively charged unilamellar vesicles into so-called disc-like micelles. Rh-Epo association to the negatively charged head groups via lysine and arginine initiates this transformation. At physiological temperatures, conformational changes within the rh-Epo structure expose a defined amino-acid sequence, which is able to induce the formation of discoid membrane structures. Enzymatic digestion, analysis, and isolation of related peptides by rp-HPLC and characterization by MS/MS enabled the identification of the membrane-affecting domain of rh-Epo (MAD-E) that represents the exposed helix B of rh-Epo. Finally, association studies performed with these peptides confirmed that the MAD-E is responsible for the formation of disc-like micelles. Since this helix B of rh-Epo has recently been supposed to be involved in the activation of neuroprotective pathways, we believe that the membrane-transforming capacity of rh-Epo participates in the proliferative activity of rh-Epo.


Langmuir | 2018

Tunable DNA Hybridization Enables Spatially and Temporally Controlled Surface-Anchoring of Biomolecular Cargo

Roland Hager; Andreas Arnold; Eva Sevcsik; Gerhard J. Schütz; Stefan Howorka

The controlled immobilization of biomolecules onto surfaces is relevant in biosensing and cell biological research. Spatial control is achieved by surface-tethering molecules in micro- or nanoscale patterns. Yet, there is an increasing demand for temporal control over how long biomolecular cargo stays immobilized until released into the medium. Here, we present a DNA hybridization-based approach to reversibly anchor biomolecular cargo onto micropatterned surfaces. Cargo is linked to a DNA oligonucleotide that hybridizes to a sequence-complementary, surface-tethered strand. The cargo is released from the substrate by the addition of an oligonucleotide that disrupts the duplex interaction via toehold-mediated strand displacement. The unbound tether strand can be reloaded. The generic strategy is implemented with small-molecule or protein cargo, varying DNA sequences, and multiple surface patterning routes. The approach may be used as a tool in biological research to switch membrane proteins from a locally fixed to a free state, or in biosensing to shed biomolecular receptors to regenerate the sensor surface.

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Gerhard J. Schütz

Vienna University of Technology

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Mario Brameshuber

Vienna University of Technology

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Karl Lohner

Austrian Academy of Sciences

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Andreas Arnold

Vienna University of Technology

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Benedikt K. Rossboth

Vienna University of Technology

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Christina Manner

Vienna University of Technology

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Gerhard J. Schuetz

Vienna University of Technology

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Julian Weghuber

Johannes Kepler University of Linz

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Martin Fölser

Vienna University of Technology

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