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Dive into the research topics where Éverson Miguel Bianco is active.

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Featured researches published by Éverson Miguel Bianco.


Marine Drugs | 2010

Effect of Elatol, Isolated from Red Seaweed Laurencia dendroidea, on Leishmania amazonensis

Adriana Oliveira dos Santos; Phercyles Veiga-Santos; Tânia Ueda-Nakamura; Benedito Prado Dias Filho; Daniela Bueno Sudatti; Éverson Miguel Bianco; Renato Crespo Pereira; Celso Vataru Nakamura

In the present study, we investigated the antileishmanial activity of sesquiterpene elatol, the major constituent of the Brazilian red seaweed Laurencia dendroidea (Hudson) J.V. Lamouroux, against L. amazonensis. Elatol after 72 h of treatment, showed an IC50 of 4.0 μM and 0.45 μM for promastigote and intracellular amastigote forms of L. amazonensis, respectively. By scanning and transmission electron microscopy, parasites treated with elatol revealed notable changes compared with control cells, including: pronounced swelling of the mitochondrion; appearance of concentric membrane structures inside the organelle; destabilization of the plasma membrane; and formation of membrane structures, apparently an extension of the endoplasmic reticulum, which is suggestive of an autophagic process. A cytotoxicity assay showed that the action of the isolated compound is more specific for protozoa, and it is not toxic to macrophages. Our studies indicated that elatol is a potent antiproliferative agent against promastigote and intracellular amastigote forms, and may have important advantages for the development of new anti-leishamanial chemotherapies.


Marine Drugs | 2011

4-Acetoxydolastane Diterpene from the Brazilian Brown Alga Canistrocarpus cervicornis as Antileishmanial Agent

Adriana Oliveira dos Santos; Elizandra Aparecida Britta; Éverson Miguel Bianco; Tania Ueda-Nakamura; Benedito Prado Dias Filho; Renato Crespo Pereira; Celso Vataru Nakamura

Natural marine products have shown an interesting array of diverse and novel chemical structures with potent biological activities. Our study reports the antiproliferative assays of crude extracts, fraction and pure compound (4R,9S,14S)-4α-acetoxy-9β,14α-dihydroxydolast-1(15),7-diene (1) obtained from brown alga Canistrocarpus cervicornis showing the antileishmanial activity. We showed that 1 had a dose-dependent activity during 72 h of treatment, exhibiting IC50 of 2.0 μg/mL, 12.0 μg/mL, and 4.0 μg/mL for promastigote, axenic amastigote and intracellular amastigote forms of Leishmania amazonensis, respectively. A cytotoxicity assay showed that the action of the isolated compound 1 was 93.0 times less toxic to the macrophage than to the protozoan. Additionally, compound 1 induced ultrastructural changes, including extensive mitochondrial damage; decrease in Rh123 fluorescence, suggesting interference with the mitochondrial membrane potential; and lipid peroxidation in parasite cells. The use of 1 from C. cervicornis against L. amazonensis parasites might be of great interest as a future alternative to the development of new antileishmanial drugs.


Journal of Thrombosis and Thrombolysis | 2011

Anticoagulation and antiplatelet effects of a dolastane diterpene isolated from the marine brown alga Canistrocarpus cervicornis

Laura de Andrade Moura; Éverson Miguel Bianco; Renato Crespo Pereira; Valéria Laneuville Teixeira; André L. Fuly

Marine brown algae of the family Dictyotaceae are rich sources of monocyclic, bicyclic, and tricyclic diterpenes. These molecules are responsible for a wide range of pharmacological and ecological functions, as antitumor and antiviral. Here, we analyzed the effect of the dolastane diterpene (4R, 9S, 14S)-4α-Acetoxy-9β,14α-dihydroxydolast-1(15),7-diene, isolated from the marine brown alga, Canistrocarpus cervicornis on blood clotting and platelet aggregation. The dolastane diterpene was able to inhibit either plasma or fibrinogen coagulation induced by thrombin as well as delayed coagulation in the recalcification test. The dolastane diterpene impaired, in a concentration-dependent manner platelet aggregation induced by collagen or adenosine diphosphate with no lysis on such cells. Thus, the dolastane diterpene maybe a promising source of natural inhibitors for hemostatic disturbs (clotting and platelet aggregation) leading to the discovery of drugs of potential use as antithrombotic and antiplatelet. In addition, the dolastane diterpene may be used as a molecular model for development of new antithrombotic agents giving new approaches to the management to the treatment of thrombotic disturbs.


Molecules | 2013

Anti-Infective Potential of Marine Invertebrates and Seaweeds from the Brazilian Coast

Éverson Miguel Bianco; Simone Quintana de Oliveira; Caroline Rigotto; Maiko L. Tonini; Tatiana da Rosa Guimarães; Francine Bittencourt; Lidiane Gouvêa; Cassandra Aresi; Maria Tereza Rojo de Almeida; Maria Izabel G. Moritz; Cintia Dalcuche Leal Martins; Fernando Scherner; João L. F. Carraro; Paulo Antunes Horta; Flávio Henrique Reginatto; Mário Steindel; Cláudia Maria Oliveira Simões; Eloir Paulo Schenkel

This manuscript describes the evaluation of anti-infective potential in vitro of organic extracts from nine sponges, one ascidian, two octocorals, one bryozoan, and 27 seaweed species collected along the Brazilian coast. Antimicrobial activity was tested against Staphylococcus aureus (ATCC 25923), Enterococcus faecalis (ATCC 29212), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922) and Candida albicans (ATCC 10231) by the disk diffusion method. Antiprotozoal activity was evaluated against Leishmania braziliensis (MHOM/BR/96/LSC96-H3) promastigotes and Trypanosoma cruzi (MHOM/BR/00/Y) epimastigotes by MTT assay. Activity against intracellular amastigotes of T. cruzi and L. brasiliensis in murine macrophages was also evaluated. Antiviral activity was tested against Herpes Simplex Virus type 1 (HSV-1, KOS strain) by the plaque number reduction assay (IC50). Cytotoxicity on VERO cells was evaluated by the MTT assay (CC50). The results were expressed as SI = CC50/IC50. The most promising antimicrobial results were obtained against S. aureus and C. albicans with Dragmacidon reticulatum. Among the seaweeds, only Osmundaria obtusiloba showed moderate activity against P. aeruginosa. Concerning antiprotozoal activity, Bugula neritina, Carijoa riseii, Dragmaxia anomala and Haliclona (Halichoclona) sp. showed the most interesting results, mainly against extracellular promastigote forms of L. braziliensis (66, 35.9, 97.2, and 43.6% inhibition, respectively). Moreover, six species of seaweeds Anadyomene saldanhae, Caulerpa cupressoides, Canistrocarpus cervicornis, Dictyota sp., Ochtodes secundiramea, and Padina sp. showed promising results against L. braziliensis (87.9, 51.7, 85.9, 93.3, 99.7, and 80.9% inhibition, respectively), and only Dictyota sp. was effective against T. cruzi (60.4% inhibition). Finally, the antiherpes activity was also evaluated, with Haliclona (Halichoclona) sp. and Petromica citrina showing the best results (SI = 11.9 and SI > 5, respectively). All the active extracts deserve special attention in further studies to chemically characterize the bioactive compounds, and to perform more refined biological assays.


PLOS ONE | 2012

Screening of Dengue Virus Antiviral Activity of Marine Seaweeds by an In Situ Enzyme-Linked Immunosorbent Assay

Andrea Cristine Koishi; Paula Rodrigues Zanello; Éverson Miguel Bianco; Juliano Bordignon; Claudia Nunes Duarte dos Santos

Dengue is a significant public health problem worldwide. Despite the important social and clinical impact, there is no vaccine or specific antiviral therapy for prevention and treatment of dengue virus (DENV) infection. Considering the above, drug discovery research for dengue is of utmost importance; in addition natural marine products provide diverse and novel chemical structures with potent biological activities that must be evaluated. In this study we propose a target-free approach for dengue drug discovery based on a novel, rapid, and economic in situ enzyme-linked immunosorbent assay and the screening of a panel of marine seaweed extracts. The in situ ELISA was standardized and validated for Huh7.5 cell line infected with all four serotypes of DENV, among them clinical isolates and a laboratory strain. Statistical analysis showed an average S/B of 7.2 and Z-factor of 0.62, demonstrating assay consistency and reliability. A panel of fifteen seaweed extracts was then screened at the maximum non-toxic dose previously determined by the MTT and Neutral Red cytotoxic assays. Eight seaweed extracts were able to reduce DENV infection of at least one serotype tested. Four extracts (Phaeophyta: Canistrocarpus cervicornis, Padina gymnospora; Rhodophyta: Palisada perforate; Chlorophyta: Caulerpa racemosa) were chosen for further evaluation, and time of addition studies point that they might act at an early stage of the viral infection cycle, such as binding or internalization.


Molecules | 2014

The in Vitro Biological Activity of the Brazilian Brown Seaweed Dictyota mertensii against Leishmania amazonensis

Amanda Silva dos Santos Aliança; Keicyanne Fernanda Lessa dos Anjos; Thiago Nogueira de Vasconcelos Reis; Taciana Mirely Maciel Higino; Maria Carolina Accioly Brelaz-de-Castro; Éverson Miguel Bianco; Regina C. B. Q. Figueiredo

Seaweeds present a wide variety of interesting bioactive molecules. In the present work we evaluated the biological activity of the dichloromethane/methanol (2:1) extract (DME) from the brown seaweed Dictyota mertensii against Leishmania amazonensis and its cytotoxic potential on mammalian cells. The extract showed significant inhibitory effect on the growth of promastigote forms (IC50 = 71.60 μg/mL) and low toxicity against mammalian cells (CC50 = 233.10 μg/mL). The DME was also efficient in inhibiting the infection in macrophages, with CC50 of 81.4 μg/mL and significantly decreased the survival of amastigote forms within these cells. The selectivity index showed that DME was more toxic to both promastigote (SI = 3.25) and amastigote (SI = 2.86) forms than to macrophages. Increased NO production was observed in treated macrophages suggesting that besides acting directly on the parasites, the DME also shows an immunomodulatory effect on macrophages. Drastic ultrastructural alterations consistent with loss of viability and cell death were observed in treated parasites. Confocal microscopy and cytometry analyzes showed no significant impairment of plasma membrane integrity, whereas an intense depolarization of mitochondrial membrane could be observed by using propidium iodide and rhodamine 123 staining, respectively. The low toxicity to mammalian cells and the effective activity against promastigotes and amastigotes, point to the use of DME as a promising agent for the treatment of cutaneous leishmaniasis.


Marine Drugs | 2013

Anti HSV-1 Activity of Halistanol Sulfate and Halistanol Sulfate C Isolated from Brazilian Marine Sponge Petromica citrina (Demospongiae)

Tatiana da Rosa Guimarães; Carlos Guillermo Quiroz; Caroline Rigotto; Simone Quintana de Oliveira; Maria Tereza Rojo de Almeida; Éverson Miguel Bianco; Maria Izabel Goulart Moritz; João L. F. Carraro; Jorge A. Palermo; Gabriela M. Cabrera; Eloir Paulo Schenkel; Flávio Henrique Reginatto; Cláudia Maria Oliveira Simões

The n-butanol fraction (BF) obtained from the crude extract of the marine sponge Petromica citrina, the halistanol-enriched fraction (TSH fraction), and the isolated compounds halistanol sulfate (1) and halistanol sulfate C (2), were evaluated for their inhibitory effects on the replication of the Herpes Simplex Virus type 1 (HSV-1, KOS strain) by the viral plaque number reduction assay. The TSH fraction was the most effective against HSV-1 replication (SI = 15.33), whereas compounds 1 (SI = 2.46) and 2 (SI = 1.95) were less active. The most active fraction and these compounds were also assayed to determine the viral multiplication step(s) upon which they act as well as their potential synergistic effects. The anti-HSV-1 activity detected was mediated by the inhibition of virus attachment and by the penetration into Vero cells, the virucidal effect on virus particles, and by the impairment in levels of ICP27 and gD proteins of HSV-1. In summary, these results suggest that the anti-HSV-1 activity of TSH fraction detected is possibly related to the synergic effects of compounds 1 and 2.


Marine Drugs | 2014

Polyoxygenated Steroids from the Octocoral Leptogorgia punicea and in Vitro Evaluation of Their Cytotoxic Activity

Maria Izabel G. Moritz; Lucas Lourenço Marostica; Éverson Miguel Bianco; Maria Tereza Rojo de Almeida; João L. F. Carraro; Gabriela M. Cabrera; Jorge A. Palermo; Cláudia Maria Oliveira Simões; Eloir Paulo Schenkel

Five new polyoxygenated marine steroids—punicinols A–E (1–5)—were isolated from the gorgonian Leptogorgia punicea and characterized by spectroscopic methods (IR, MS, 1H, 13C and 2-D NMR). The five compounds induced in vitro cytotoxic effects against lung cancer A549 cells, while punicinols A and B were the most active, with IC50 values of 9.7 μM and 9.6 μM, respectively. The synergistic effects of these compounds with paclitaxel, as well as their effects on cell cycle distribution and their performance in the clonogenic assay, were also evaluated. Both compounds demonstrated significant synergistic effects with paclitaxel.


Biomedicine & Pharmacotherapy | 2018

TSH fraction from Petromica citrina: A potential marine natural product for the treatment of sepsis by Methicillin-resistant Staphylococcus aureus (MRSA)

Larissa Constantino; Tatiana da Rosa Guimarães; Simone Quintana de Oliveira; Éverson Miguel Bianco; Luiza Gabriela de Souza Pessoa; Monique Michels; Eloir Paulo Schenkel; Felipe Dal Pizzol; Flávio Henrique Reginatto

BACKGROUND AND PURPOSE Antibiotic resistance is now a worldwide public health problem. A potential alternative source in the search for new antibiotics is the bioactive molecules obtained from marine products, as the halistanol trisulfate, obtained from Petromica citrina, and herein investigated from its antimicrobial and anti-inflammatory properties. EXPERIMENTAL APPROACH The antimicrobial activity of the fractionation products (TSH fraction, halistanol sulfate (HS) and halistanol sulfate C (HS-C)) of the marine sponge Petromica citrina was evaluated against twenty bacteria and two fungi strains by the disk diffusion and microdilution methods. After initial in vitro tests, an in vivo assay was proposed, to evaluate survival and inflammatory parameters in an animal model of peritonitis mediated by MRSA. The animals are treated with TSH fraction (1, 2.5 and 5 mg kg-1) or Vancomycin (30 mg kg-1) twice (6 and 18 h after induction) until organ removal for evaluation of the inflammatory profile, or for 3 days, 12 h each (6 h, 18 h, 30 h, 42 h, 54 h and 66 h after induction) in animals which were followed-up for by five days, for the evaluation of survival. KEY RESULTS The BF fraction, TSH fraction, HS and HS-Cinhibited, in vitro, the Enterococcus faecalis, Staphylococcus aureus and Candida albicans growth. Moreover, these samples were effective against S. aureus (MSSA), MRSA and Vancomycin-Resistence Enterococcus (VRE). The in vivo results demonstrated that TSH fraction reduced mortality when compared to the saline group. To evaluate the role of inflammation in outcomes of peritonitis, cytokines (IL-1β, IL-6, TNF-α) and MPO activity were measured. In general, anti-inflammatory activity was detected in animals treated with TSH in different doses. CONCLUSION AND IMPLICATIONS These data suggest that TSH may be an interesting alternative for the treatment infections by Gram-positive resistant bacteria, due to its antimicrobial profile associated with its anti-inflammatory properties.


Planta Medica | 2009

Effect of the marine brown alga Canistrocarpus cervicornis on promastigote forms of Leishmania (L.) amazonensis

Adriana Oliveira dos Santos; Elizandra Aparecida Britta; Tânia Ueda-Nakamura; B.P. Dias Filho; Éverson Miguel Bianco; Vl Teixeira; Rodrigues Pereira; Cv Nakamura

Leishmaniasis is a disease resulting from infection by protozoan parasites of the genus Leishmania. Pentavalent antimonials, used clinically for more than 50 years, are still the first-choice drugs for the treatment of leishmaniasis, but they are toxic, require long-term treatment, and are prone to stimulate drug resistance [1]. Marine brown algae (Phaeophyceae) belonging to the order Dictyotales have emerged as an exceptionally rich source of diterpenoids, which form part of a defensive strategy against herbivores in the marine environment [2]. We have investigated the activity of crude extracts, a fraction, and an isolated compound (4R, 9S, 14S)-4α-Acetoxy-9β,14α-dihydroxydolast-1(15),7-diene of the brown alga Canistrocarpus cervicornis against promastigote forms of Leishmania amazonensis. The antiproliferative assays showed a dose-dependent effect against promastigotes with IC50 values in the range between 20.0 and 80.0µg/mL for crude extracts, 5.0µg/mL for the fraction and 2.0µg/mL for the isolated compound from C. cervicornis. We also investigated targets in the parasite by means of electron microscopy. Ultrastructural alterations were mainly observed in the mitochondrion of parasites treated with the isolated compound. Based on the current study, compounds from C. cervicornis appear to be an alternative for the development of new antiparasitic chemotherapies. However, further in vitro and in vivo studies are necessary to elucidate the mechanism of action of this compound. Acknowledgements: CNPq, FINEP, PRONEX/Fundacao Araucaria. References: [1] Croft, S.L. et al. (2006) Indian J. Med. Res. 123:399–410. [2] Garcia, D.G. et al. (2009) Phytother. Res., in press.

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Renato Crespo Pereira

Federal Fluminense University

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Celso Vataru Nakamura

Universidade Estadual de Maringá

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Tânia Ueda-Nakamura

Universidade Estadual de Maringá

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Maria Tereza Rojo de Almeida

Facultad de Ciencias Exactas y Naturales

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Adriana Oliveira dos Santos

Universidade Estadual de Londrina

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Benedito Prado Dias Filho

Universidade Estadual de Maringá

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Suzi Meneses Ribeiro

Federal University of Rio de Janeiro

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Valéria Laneuville Teixeira

Federal University of Rio de Janeiro

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Gabriela M. Cabrera

Facultad de Ciencias Exactas y Naturales

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Jorge A. Palermo

Facultad de Ciencias Exactas y Naturales

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