Evgeny Minin
University of Münster
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Featured researches published by Evgeny Minin.
Virchows Archiv | 2008
Daniel Palmes; Andree Zibert; Tymotheus Budny; Ralf Bahde; Evgeny Minin; Linus Kebschull; Jens Peter Hölzen; Hartmut Schmidt; Hans-Ullrich Spiegel
The remarkable capacity of the liver to regenerate after injury and the prospects of organ self-renewal have attracted much interest in the understanding and modulation of the underlying molecular events. We investigated the effect of mammalian target of rapamycin (mTOR) inhibitor rapamycin (RAPA) on liver by correlating intravital microscopy, immunohistochemistry, and reverse transcriptase polymerase chain reaction in a rat model of 2/3 hepatectomy. RAPA significantly retarded proliferation of hepatocytes, endothelial cells, and hepatic stellate cells (HSCs) mostly between days 2 and 4 after hepatectomy and down-regulated major cytokines and growth factors (tumor necrosis factor alpha, hepatocyte growth factor, platelet-derived growth factor, platelet-derived growth factor receptor, insulin-like growth factor-1, transforming growth factor beta 1) important for liver regeneration. These effects were almost absent at later time points. RAPA also had a transient, but broad effect on angiogenesis, and impaired sinusoidal density as well as mRNA levels of vascular endothelial growth factor, vascular endothelial growth factor receptor 1, vascular endothelial growth factor receptor 2, and angiopoietin-1. Activation of HSC was also transiently suppressed as observed by smooth muscle protein 1 alpha protein expression and intercellular adhesion molecule-1 mRNA levels. The rate of apoptosis in liver was significantly increased by RAPA between day 3 and day 7. The effect of RAPA on liver repair, angiogenesis, and HSC activation is confined to the phase of active cell proliferation. This transient effect might allow further exploration of mTOR inhibitors in clinical situations that involve liver regeneration, and seems to have implications beyond immunosuppression.
Journal of Surgical Research | 2008
Jens Peter Hölzen; Christian August; Ralf Bahde; Evgeny Minin; Detlef Lang; Stefan Heidenreich; Karl-Heinz Dietl; Hans-Ullrich Spiegel
BACKGROUND Cytoprotective proteins, such as heme oxygenase-1 (HO-1), play a decisive role in ischemia-reperfusion injury during kidney transplantation. The aim of this study was to investigate the impact of heme oxygenase-1 on microcirculation and on ischemia-reperfusion injury in an isogenic kidney transplantation rat model. MATERIALS AND METHODS Seventy male Lewis rats were distributed into three groups. In Group 1(control), the kidneys were only mobilized. In Groups 2 and 3, bilateral nephrectomy was performed, and a kidney from another Lewis rat was orthotopically transplanted on the left side. The donor animals in Group 3 received preconditioning with the HO-1 inductor hemin. 24 h after reperfusion graft function and morphology were examined. Microcirculation was investigated by in vivo microscopy of the renal surface 1 h after reperfusion. RESULTS HO-1 preconditioning led to significantly lower serum creatinine and serum urea, as well as less histological damage and inducible nitric oxide synthase expression. Microcirculation was improved by a significant enlargement of the vascular diameter and an increase of the capillary flow. CONCLUSIONS Treatment with hemin improves microcirculation by induction of HO-1 and reduces ischemia-reperfusion injury after kidney transplantation. HO-1 induction was shown to be a promising approach in the preconditioning of donor kidneys.
Acta Neuropathologica | 2006
Igor B. Buchwalow; Evgeny Minin; Frank-Ulrich Müller; Geertje Lewin; Vera E. Samoilova; Wilhelm Schmitz; Maren Wellner; Martin Hasselblatt; Karla Punkt; Ursula Müller-Werdan; Uta Demus; Jan Slezak; Gabriele Koehler; Werner Boecker
Duchenne and Becker muscular dystrophies (DMD and BMD) are associated with decreased total nitric oxide (NO). However, mechanisms leading to NO deficiency with consequent muscle-cell degeneration remain unknown. To address this issue, we examined skeletal muscles of DMD and BMD patients for co-expression of NO synthase (NOS) with nitrotyrosine and transcription factor CREB, as well as with enzymes engaged in NO signaling. Employing immunocytochemical labeling, Western blotting and RT-PCR, we found that, in contrast to the most commonly accepted view, neuronal NOS was not restricted to the sarcolemma and that muscles of DMD and BMD patients retained all three NOS isoforms with an up-regulation of the inducible NOS isoform, CREB and nitrotyrosine. We suggest that enhanced nitrotyrosine immunostaining in muscle fibers as well as in the vasculature of DMD and BMD specimens reflects massive oxidative stress, resulting in withdrawal of NO from its regular physiological course via the scavenging actions of superoxides.
Journal of Investigative Surgery | 2009
Daniel Palmes; Heiner Wolters; Hans-Ullrich Spiegel; Erhard M¨ller; Evgeny Minin; Hans Peter Heistermann
ABSTRACT Major bile duct lesions are usually treated by a hepaticojejunostomy which is often complicated by cholangitis and liver fibrosis. The aim of this study was to investigate the morphologic features of a neo-bile duct created from a vein and a biodegradable endoluminal stent. The neo-bile duct was created using a segment of the external jugular vein which was endoluminally stented by a biodegradable poly-lactate-acid stent. In 18 pigs, the common bile duct was resected and replaced by the vein with (n = 12) or without endoluminal stent (n = 6). Six animals served as controls. Survival, liver function and morphological changes of the neo-bile duct and the liver were observed for six months. After six months, the neo-bile duct morphologically resembled the native bile duct showing Ck7-positive columnar epithelium and newly formed capillaries in the bile duct wall. The biodegradable stent disappeared after four months. All animals survived and showed normal liver function and no cholestasis. In contrast, after sole vein reconstruction of the bile duct, four animals died due to biliary peritonitis and cholangitis. Creation of a neo-bile duct which morphologically resembles the native bile duct is feasible by using a bodys own vein and a biodegradable endoluminal stent.
Liver International | 2011
Ralf Bahde; Linus Kebschull; Sandra Stöppeler; Andree Zibert; Ramsi Siaj; Jens Peter Hölzen; Evgeny Minin; Hartmut Schmidt; Hans-Ullrich Spiegel; Daniel Palmes
Background: The regeneration capacity of cirrhotic livers might be affected by angiotensin‐1 (AT1) receptors located on hepatic stellate cells (HSC). The effect of AT1 receptor blockade on microcirculation, fibrosis and liver regeneration was investigated.
Archive | 2007
Ralf Bahde; Daniel Palmes; Oliver Gemsa; Evgeny Minin; H. de Groot; U. Rauen; Hans-Ullrich Spiegel
Optimal organ preservation remains a continuous problem in liver transplantation and preservation quality is a main factor influencing graft failure. Based on new insights in pathophysiologic mechanisms modifications of the HTK solution have been made to reduce cold-storage injury (histidine was partially substituted by N-acetyl-histidine, chloride was replaced by aspartate and lactobionate and glycine, alanine and arginine were supplemented).
Virchows Archiv | 2005
Daniel Palmes; Evgeny Minin; Tymoteusz Budny; Dirk Uhlmann; Barbara Armann; Udo Stratmann; Hermann Herbst; Hans-Ullrich Spiegel
Biochemical and Biophysical Research Communications | 2005
Igor B. Buchwalow; Evgeny Minin; Vera E. Samoilova; Werner Boecker; Maren Wellner; Wilhelm Schmitz; Joachim Neumann; Karla Punkt
Acta Histochemica | 2005
Igor B. Buchwalow; Evgeny Minin; Werner Boecker
Journal of Hepatology | 2005
Daniel Palmes; Sebastian Skawran; Udo Stratmann; Barbara Armann; Evgeny Minin; Hermann Herbst; Hans-Ullrich Spiegel