Evgeny V. Lyubin

Cellular viscoelasticity probed by active rheology in optical tweezers

Abstract. A novel approach to probe viscoelastic properties of cells based on double trap optical tweezers is reported. Frequency dependence of the tangent of phase difference in the movement of the opposite erythrocyte edges while one of the edges is forced to oscillate by optical tweezers appeared to be highly dependent on the rigidity of the cellular membrane. Effective viscoelastic parameters characterizing red blood cells with different stiffnesses (normal and glutaraldehyde-fixed) are determined. It is shown that the photo-induced effects caused by laser trapping at the power level used in the experiments are negligible giving the possibility to use the offered technique for dynamic monitoring of soft materials viscoelastic properties.

Optical tweezers study of red blood cell aggregation and disaggregation in plasma and protein solutions

Abstract. Kinetics of optical tweezers (OT)-induced spontaneous aggregation and disaggregation of red blood cells (RBCs) were studied at the level of cell doublets to assess RBC interaction mechanics. Measurements were performed under in vitro conditions in plasma and fibrinogen and fibrinogen + albumin solutions. The RBC spontaneous aggregation kinetics was found to exhibit different behavior depending on the cell environment. In contrast, the RBC disaggregation kinetics was similar in all solutions qualitatively and quantitatively, demonstrating a significant contribution of the studied proteins to the process. The impact of the study on assessing RBC interaction mechanics and the protein contribution to the reversible RBC aggregation process is discussed.

Direct measurements of forces induced by Bloch surface waves in a one-dimensional photonic crystal

An experimental study of the interaction between a single dielectric microparticle and the evanescent field of the Bloch surface wave in a one-dimensional (1D) photonic crystal is reported. The Bloch surface wave-induced forces on a 1 μm polystyrene sphere were measured by photonic force microscopy. The results demonstrate the potential of 1D photonic crystals for the optical manipulation of microparticles and suggest a novel approach for utilizing light in lab-on-a-chip devices.

Composite SERS-based satellites navigated by optical tweezers for single cell analysis

Herein, we have designed composite SERS-active micro-satellites, which exhibit a dual role: (i) effective probes for determining cellular composition and (ii) optically movable and easily detectable markers. The satellites were synthesized by the layer-by-layer assisted decoration of silica microparticles with metal (gold or silver) nanoparticles and astralen in order to ensure satellite SERS-based microenvironment probing and satellite recognition, respectively. A combination of optical tweezers and Raman spectroscopy can be used to navigate the satellites to a certain cellular compartment and probe the intracellular composition following cellular uptake. In the future, this developed approach may serve as a tool for single cell analysis with nanometer precision due to the multilayer surface design, focusing on both extracellular and intracellular studies.

Trap position control in the vicinity of reflecting surfaces in optical tweezers

Shift of the trap position from the laser beam waist of optical tweezers is studied experimentally in the presence of a reflecting surface in the vicinity of the focal plane. A standing wave is formed owing to the interference of waves forming the waist and reflected from the surface. The standing wave is shown to affect significantly the resulting trap position. The distance between the surface and the stable optical trap as a function of the trapped particle size is studied numerically. A new method to stabilize the position of the microparticle relative to the surface is proposed. The localization accuracy is determined by the Brownian fluctuations in optical tweezers and is about 10 nm for effective trap stiffness of 4 × 10−5 N/m.

Probing of pair interaction of magnetic microparticles with optical tweezers

Magnetic interaction of paramagnetic Brownian submicron-sized particles is studied by optical tweezers technique. Correlation analysis allows one to extract magnetic interaction of two particles 0.4 μm in size, which are optically trapped at the distance of 3 μm one from each other and placed in a static magnetic field of 30 Oe, from the background of their Brownian motion. The magnetic interaction force is estimated to be of approximately 100 fN. Two configurations of the mutual orientation of the magnetic field vector and the line connecting two centers of optical traps are used in the experiment. For field vector orientation parallel/perpendicular to this line, the magnetic interaction is detected by the cross-correlation function increase/decrease in comparison with the absence of magnetic field on the time scales of 1 ms.

Near-field probing of Bloch surface waves in a dielectric multilayer using photonic force microscopy

The potential of photonic force microscopy (PFM) for probing the optical near-field in the vicinity of a dielectric multilayer is demonstrated. An experimental study of Bloch surface waves (BSWs) using PFM is described in detail. The applied technique is based on measuring the BSW-induced gradient force acting on a probe particle combined with precise control of the distance between the particle and the multilayer surface. The BSW-induced potential profile measured using PFM is presented. The force interaction between the probe and the BSW evanescent field is numerically studied. The results indicate that a polystyrene particle with a diameter of 1 μm does not significantly perturb the BSW field and can be used to probe the optical near-field intensity in an elegant, noninvasive manner.

Direct measurements of magnetic interaction-induced cross-correlations of two microparticles in Brownian motion

The effect of magnetic interactions on the Brownian motion of two magnetic microparticles is investigated. The cross-correlations of the thermal fluctuations of the two magnetic microbeads are directly measured using double-trap optical tweezers. It is experimentally demonstrated that the cross-correlation function is governed by the gradient of the magnetic force between the microparticles. The magnetic forces are measured with femtonewton precision, and the magnetic dipole moments of individual microparticles are determined within an accuracy on the order of fA-m2.

Detection of Brownian Torque in a Magnetically-Driven Rotating Microsystem

Thermal fluctuations significantly affect the behavior of microscale systems rotating in shear flow, such as microvortexes, microbubbles, rotating micromotors, microactuators and other elements of lab-on-a-chip devices. The influence of Brownian torque on the motion of individual magnetic microparticles in a rotating magnetic field is experimentally determined using optical tweezers. Rotational Brownian motion induces the flattening of the breakdown transition between the synchronous and asynchronous modes of microparticle rotation. The experimental findings regarding microparticle rotation in the presence of Brownian torque are compared with the results of numerical Brownian dynamics simulations.

Photonic force microscopy of surface electromagnetic waves in a one-dimensional photonic crystal

The potential of photonic force microscopy (PFM) to directly probe the field of the Bloch surface wave (BSW) in a one-dimensional photonic crystal is considered. Optical forces acting on a dielectric microparticle in the evanescent field of the BSW are estimated in the dipole approximation and calculated by finite-difference time-domain (FDTD) analysis. Technical details of the PFM measurements are described.