Ewald Glossner

A PC-at based video device for flow cytometrically triggered cell imaging in flow.

A personal computer-based system of imaging in flow is described which takes cell images directly from the transducer of a flow cytometer. Imaging is triggered by the pulses of the flow system. The movement of the cells is frozen by ultra-short flashes from pulsed light emitting diodes (LED). The images are taken up by a video camera, transferred to an add-in board of the PC and then stored on disk. A novel method has been developed for capturing the video images which normally arise in asynchronism with the video frame. Images of different cells and particles exposed during flow analysis are shown.