F Buono
University of Pisa
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Publication
Featured researches published by F Buono.
Biochemical Journal | 2004
Mario Cappiello; Alessandra Lazzarotti; F Buono; Andrea Scaloni; Chiara D'Ambrosio; Pietro Amodeo; Blanca López Méndez; Paolo Pelosi; Antonella Del Corso; Umberto Mura
A manganese-dependent cysteinyl-glycine hydrolysing activity has been purified to electrophoretic homogeneity from bovine lens. The characterization of the purified enzyme (molecular mass of the native protein, molecular mass of the subunit and extensive primary structure analysis) allowed the unequivocal attribution of the cysteinyl-glycine hydrolysing activity, which is usually associated with alanyl aminopeptidase (EC 3.4.11.2) or membrane-bound dipeptidase (EC 3.4.13.19), to LAP (leucyl aminopeptidase; EC 3.4.11.1). Analysis of the pH dependence of Cys-Gly hydrolysis catalysed by LAP, supported by a molecular modelling approach to the enzyme-substrate conformation, gave insights into the ability of the enzyme to recognize Cys-Gly as a substrate. Due to the effectiveness of LAP in hydrolysing Cys-Gly (K(m)=0.57 mM, kcat=6.0x10(3) min(-1) at pH 7.4 and 25 degrees C) with respect to other dipeptide substrates, a new role for this enzyme in glutathione turnover is proposed.
Experimental Eye Research | 2000
Antonella Del Corso; Luca Costantino; Giulio Rastelli; F Buono; Umberto Mura
Biochemical Journal | 1998
Dal Monte M; I. Cecconi; F Buono; P.G. Vilardo; Del Corso A; Umberto Mura
Journal of Biochemical and Biophysical Methods | 2006
Antonella Del Corso; Mario Cappiello; F Buono; Roberta Moschini; Aldo Paolicchi; Umberto Mura
Investigative Ophthalmology & Visual Science | 2001
Umberto Mura; Mario Cappiello; I Cecconi; F Buono; M Dal Monte; S Banditelli; Roberta Moschini; I Marini; A Del Corso
Archive | 2003
Umberto Mura; Mario Cappiello; Del Corso A; F Buono
Italian Journal of Biochemistry | 2002
F Buono; Gm De Donatis; Mario Cappiello; A Del Corso; Umberto Mura
45° Congresso Nazionale SIB | 2000
S Banditelli; Enrico Boldrini; I Cecconi; Roberta Moschini; F Buono; Catia Barsotti; Massimo Dal Monte; Mario Cappiello; Antonella Del Corso; Umberto Mura
Riunione Annuale Sezione Toscana-Umbria-Marche Società Italiana di Biochimica | 1999
Pg Vilardo; Mario Cappiello; I Cecconi; M Dal Monte; S Banditelli; Roberta Moschini; Catia Barsotti; F Buono; I Marini; A Del Corso; Umberto Mura
XII Convegno Nazionale del Gruppo Struttura e Funzione delle Proteine | 1997
Massimo Dal Monte; I Cecconi; F Buono; Mario Cappiello; Pg Vilardo; I Marini; Antonella Del Corso; Umberto Mura
