F.H. Jonker

Serum amyloid-A (SAA) and haptoglobin (Hp) plasma concentrations in newborn calves

The concentrations of 2 major bovine acute-phase proteins, haptoglobin (Hp) and serum amyloid-A (SAA), were measured in plasma obtained shortly after birth from 22 healthy calves. In a separate group of diseased calves (n = 8), Hp and SAA concentrations were measured to determine whether newborn calves (up to 4 d old) are able to produce SAA and Hp. In blood samples taken directly after birth, the Hp plasma concentrations were all below the limit of detection. The mean SAA concentration was independent of weight (r = 0.063), degree of acidosis (r = -0.125), sex (p > 0.05), and were not different in calves born after different types of obstetrical help (p > 0.05). In the group of diseased calves, an increased Hp concentration was measured in only 2 of 8 animals, whereas the mean SAA concentration was significantly higher (p < 0.05) than in the healthy newborn calves. These data suggest that prenatal stress due to parturition does not form a stimulus for the production of acute-phase proteins in the fetal calf. The low Hp plasma concentrations might indicate that either it takes a few days to establish a detectable concentration of this protein, or that Hp production is not fully developed in newborn calves.

Umbilical cord clamping in term piglets: A useful model to study perinatal asphyxia?

Perinatal asphyxia results in tissue and cellular changes during the reperfusion period and clinical signs like perinatal mortality and decreased vitality at birth in newborn piglets. This study aimed to develop and validate a model of birth asphyxia, mimicking the evolvement of birth asphyxia in natural farrowings by conducting umbilical cord clamping (UCC) in term piglets during caesarean sections under general anaesthesia. In total 23 piglets were subjected to 5-8min of UCC and 24 piglets served as controls. Acid-base balance values and heart rates measured before UCC remained fairly constant throughout the surgical procedure, indicating nearly identical starting conditions of piglets within and between litters. UCC resulted in a significant, mild, mixed respiratory-metabolic acidosis (pH 7.22, pCO(2) 9.8kPa, BE(ecf) 2mmol/L, lactate 6.5mmol/L; controls: pH 7.31, pCO(2) 8.5kPa, BE(ecf) 5mmol/L, lactate 4mmol/L) at 10min after birth (defined as simultaneous cutting of the umbilical cord and removal of a plastic bag that had been placed over the head to avoid air intake). Heart rates were significantly decreased during UCC (range: 83-107beats/min versus 128-134beats/min in controls). Rectal temperatures and changes in body weight until 72h of life were not affected by UCC. Interestingly, four control and seven clamped piglets did not survive as no independent respiration could be attained. Birth weights and duration of UCC of these piglets did not differ significantly from those in surviving control and clamped piglets. In conclusion the mixed respiratory-metabolic acidosis arising in the surviving clamped piglets is not as severe as can be expected in highly asphyxiated, vaginally delivered newborn piglets. Repeatability of the model is compromised by considerable variation in the individual response to UCC.

Ationship between ultrasonic characteristics of the corpus luteum, plasma progesterone concentration and early pregnancy diagnosis in friesian mares

The purpose of the present study was to evaluate the change in cross-sectional area of the early corpus luteum (CL) and progesterone production in relation to subsequent pregnancy diagnosis. The cross-sectional area of the CL of 75 Friesian brood mares was measured by ultrasonography on Day 1 or 2 and Day 8 or 9 after ovulation. The change in cross-sectional area was expressed in a volume ratio. Plasma progesterone concentrations were measured on Days 8 to 9, and ultrasonography to determine pregnancy status was carried out on Day 17. The data obtained were analyzed by using a multiple logistic regression model. There were significant differences in the age, volume ratio and progesterone concentration between pregnant and nonpregnant mares. Pregnancy on Day 17 was related to the change in size of the CL up to Days 8 to 9 and progesterone concentration on Days 8 to 9. These differences between pregnant and nonpregnant mares might reflect the first luteal response to pregnancy.

Feasibility of continuous recording of fetal heart rate in the near term bovine fetus by means of transabdominal doppler

A transabdominal Doppler technique for the recording of fetal heart rate (FHR) was investigated in the bovine fetus. During the last 2 weeks of gestation recordings were made once or twice per week in eight cows by placing a 1.5 MHz Doppler transducer on the right ventral abdominal wall. Continuous FHR recordings with a duration of more than 30 min were obtained in 29 of the 35 sessions. Major displacements of the fetus were the main cause of recording failure. The overall mean baseline FHR was 105 +/- 1.5 bpm with a range of 90 to 125 bpm. The mean bandwidth of the baseline FHR was 10.8 +/- 0.7 bpm with a range of < 5 to 20 bpm. Periods with different bandwidths alternated. The alternation of bovine FHR patterns pointed to the existence of different fetal behavioural states. The mean number of accelerations of FHR was 7.1 +/- 1.0 per hour. Many of the accelerations coincided with visible or perceptible fetal body movements. Decelerations of FHR occurred less frequently (range 0 to 4 per h). A period of tachycardia accompanied by an increased number of fetal movements occurred in 3 of the 29 FHR recordings. This non-invasive technique appears to be suitable to study FHR patterns during gestation and to investigate the presence and characteristics of behavioural states in the bovine fetus. It also provides the possibility to study effects on the fetus of drugs administered to the dam.

Fetal movements during late gestation in the pig: A longitudinal ultrasonographic study

Repeated ultrasonographic observation of fetal movements was used to distinguish movement patterns and to investigate the rate of occurrence and temporal organisation of these patterns (rest-activity cycles) during the last three weeks of gestation in the pig. By means of transabdominal ultrasonography with a 3.5MHz linear array transducer, motility in ten different fetuses (one per sow) was studied. Six (median; range 4-6) 1h recordings were made per fetus at 3-5 day intervals. Fifty-five 1h recordings were available for analysis. The occurrence of fetal general movements (GM), isolated head (HM), forelimb movements (LM), and rotations (ROT) was analysed from video tapes. For each movement pattern, the trend in occurrence over time was assessed by multilevel analysis. The temporal association between different movement patterns was studied by calculation of the kappa value. ROT occurred very infrequently and showed no particular trend over time. GM, HM, and LM showed a significant decreasing trend towards parturition (P<0.01). Total fetal activity (i.e., the sum of the four movement incidences) declined from an average of 25% of recording time to 9% over the last three weeks of pregnancy. Periods of fetal quiescence gradually increased with progressing gestation (P<0.05). There was no evidence of concordant association between the periods of rest and activity of GM, HM, and LM or of improved temporal linkage between these movement patterns with time. Fetal bodily activity decreases towards parturition mainly due to prolonged periods of rest. Fetal movement patterns show rest-activity cycles, but each pattern appears to cycle independently from the other throughout late gestation. The present results of spontaneous fetal movements in the pig provide reference data for future studies of fetal activity under different zoo technical conditions or pharmacological interventions.

Ultrasound observations of fetal movements in the pig: an exploratory study

Abstract We studied the feasibility and practical conditions of repeated, non-invasive ultrasound scanning for fetal movements during late gestation in the pig. It appeared that, after a short training period of the sow, good quality recordings of fetal movements can be obtained for 60 min when using a 3.5 MHz ultrasound transducer of 12 or 18 cm long. Four different movement patterns were discerned in the fetal pig: general movement, isolated head and (fore)limb movements, and rotation. Head movements were the predominant types of fetal activity. Preliminary data are provided on their rate of occurrence. The observed large variation in movement incidence (% of time or number per hour) was found to be related to the gestational age at recording and in part to the size of the transducer. There was evidence of a decreasing trend in fetal activity towards parturition. This exploratory study demonstrated that longitudinal study on fetal movements in the pig is feasible. This approach also permits quantitative analysis of fetal motility under different (patho-) physiological and management conditions.