F.L. Schanbacher

Electroimmunodiffusion on cellulose acetate: A rapid method for analysis of bovine lactoferrin in chromatography effluents☆

Abstract The cellulose acetate EID method is described in terms of the dynamic behavior of the system relative to electrophoresis time, antiserum concentration, and antigen concentration. As expected, the behavior of the system is virtually identical to the agarose EID system described by Laurell (4). The method has been applied to the direct quantitation of the milk iron-binding protein lactoferrin and its use in determining the elution profile of this nonenzymatic protein from chromatography effluents has been demonstrated. Guidelines are given for the development of the assay toward other proteins.