F. R. P. Souza

Evaluation of TFAM and FABP4 gene polymorphisms in three lines of Nellore cattle selected for growth.

We analyzed the polymorphisms TFAM HaeIII, TFAM MboI and FABP4 MspA1I in three Nellore lines selected for growth in order to evaluate how selection affects the frequencies of these polymorphisms and evaluate their association with growth and carcass traits in Zebu cattle. Birth, weaning and yearling weights, rump height, longissimus muscle area, backfat thickness, and rump fat thickness were analyzed. The sample was constituted of animals from two lines selected for yearling weight (NeS and NeT), and a control line (NeC), established in 1980, at the São Paulo Instituto de Zootecnia. Two hundred and seventy-two heifers were genotyped for TFAM gene SNPs, and 325 heifers were genotyped for the FABP4 SNP. High frequencies were observed for the alleles A (TFAM HaeIII), C (TFAM MboI) and C (FABP4 MspA1I). Significant differences in allele frequencies between NeS and NeT were observed for the TFAM HaeIII, and between the line NeT and lines NeC and NeS for the FABP4 MspA1I SNP. Five haplotypes were observed for the two polymorphisms in the TFAM gene, haplotype AACC being the most frequent. None of the markers separately or according to haplotype was significantly associated with the growth and carcass traits. The low frequencies of alleles that are associated with high marbling scores and thick subcutaneous fat in taurine breeds might explain the low means for these traits in Nellore cattle.

Expression of genes related to mitochondrial function in Nellore cattle divergently ranked on residual feed intake

Several measures have been proposed to investigate and improve feed efficiency in cattle. One of the most commonly used measure of feed efficiency is residual feed intake (RFI), which is estimated as the difference between actual feed intake and expected feed intake based on the animal’s average live weight. This measure permits to identify and select the most efficient animals without selecting for higher mature weight. Mitochondrial function has been indicated as a major factor that influences RFI. The analysis of genes involved in mitochondrial function is therefore an alternative to identify molecular markers associated with higher feed efficiency. This study analyzed the expression of PGC1α, TFAM, UCP2 and UCP3 genes by quantitative real-time PCR in liver and muscle tissues of two groups of Nellore cattle divergently ranked on RFI values in order to evaluate the relationship of these genes with RFI. In liver tissue, higher expression of TFAM and UCP2 genes was observed in the negative RFI group. Expression of PGC1α gene did not differ significantly between the two groups, whereas UCP3 gene was not expressed in liver tissue. In muscle tissue, higher expression of TFAM gene was observed in the positive RFI group. Expression of PGC1α, UCP2 and UCP3 genes did not differ significantly between the two groups. These results suggest the use of TFAM and UCP2 as possible candidate gene markers in breeding programs designed to increase the feed efficiency of Nellore cattle.

Principal component analysis of breeding values for growth and reproductive traits and genetic association with adult size in beef cattle1

Principal component analysis was applied to evaluate the variability and relationships among univariate breeding values predicted for 9 weaning and yearling traits, as well as suggest functions of the traits that would promote a particular breeding objective. Phenotypic and pedigree information from 600,132 Nelore animals was used. Genetic parameters and breeding values were obtained from univariate analyses of birth to weaning weight gain; weaning to yearling weight gain; conformation, finishing precocity, and muscling scores at weaning and at yearling; and yearling scrotal circumference. The principal component mainly associated with maturity (precocious vs. late animals) was used as a pseudophenotype in bivariate analyses with either adult weight or adult height of cows. Direct heritability estimates ranging from 0.19 ± 0.01 to 0.41 ± 0.01 indicate that these 9 traits are all heritable to varying degrees. Correlations between the breeding values for the various traits ranged from 0.14 to 0.88. Principal component analysis was performed on the standardized breeding values. The first 3 principal components attained the Kaiser criterion, retaining 48.06%, 18.03%, and 12.97% of the total breeding value variance, respectively. The first component was characterized by positive coefficients for all traits. The second component contrasted weaning traits with yearling traits. The third component represented a contrast between late maturity animals (better for weight gain and conformation) and early maturity animals (better for finishing precocity, muscling, and scrotal circumference). Thus, the first 3 components represent 3 different potential selection criteria. Selecting for the first principal component would identify animals with positive breeding values for all studied traits. The second principal component may be used to identify animals with higher or lower maturation rates (precocity). Animals with negative values in the third principal component are regarded as early maturing. Genetic correlations between maturity (third principal component) with adult weight and adult height were 0.19 ± 0.02 and 0.32 ± 0.02, respectively. These results showed that the weaning and yearling bovine maturity is genetically associated with the adult size of the cows used in reproduction.

Reduced-rank models of growth and reproductive traits in Nelore cattle

In beef cattle genetic evaluation, principal component models of the additive genetic effect could be used to incorporate several traits in the same analysis, without an important increase in the number of parameters to be estimated. In this study, multitrait (MT) and reduced-rank models were compared for their ability to estimate parameters and predict breeding values for weaning weight, yearling weight, weaning hip height, yearling hip height, weaning to yearling weight gain, scrotal circumference, and age at the first calving. Data obtained were from 74,388 Nelore animals, born to 1441 sires and 28,502 cows. Six analyses were performed using a MT model that incorporated all the traits simultaneously and five reduced-rank models for the genetic additive direct (co)variance matrix, fitting the first one (PC1), two (PC2), three (PC3), four (PC4), and five (PC5) principal components. The model considering the first three principal components (PC3) provided the best fit. Direct and maternal heritability and the respective standard errors obtained from the MT and PC3 models were similar. In general, the PC3 model provided slightly stronger genetic correlations between the traits when compared with those obtained with the MT model. The rank correlations between the breeding values predicted with the MT and PC3 models for the different traits ranged from 0.93 to 0.99. When 2% and 10% of the best sires were selected on the basis of breeding values predicted by the MT model, the degree of concordance with the PC3 model ranged from 86% to 97%. The first three principal components explained most of the genetic variation among animals, suggesting that major changes should not be expected in the sires classification on the basis of breeding values predicted for growth and reproductive traits. Models of principal components could be used for beef cattle genetic evaluation, especially when considering several economic traits in the same analysis.

Association of ADIPOQ, OLR1 and PPARGC1A gene polymorphisms with growth and carcass traits in Nelore cattle.

In beef cattle farming, growth and carcass traits are important for genetic breeding programs. Molecular markers can be used to assist selection and increase genetic gain. The ADIPOQ, OLR1 and PPARGC1A genes are involved in lipid synthesis and fat accumulation in adipose tissue. The objective of this study was to identify polymorphisms in these genes and to assess the association with growth and carcass traits in Nelore cattle. A total of 639 animals were genotyped by PCR-RFLP for rs208549452, rs109019599 and rs109163366 in ADIPOQ, OLR1 and PPARGC1A gene, respectively. We analyzed the association of SNPs identified with birth weight, weaning weight, female yearling weight, female hip height, male yearling weight, male hip height, loin eye area, rump fat thickness, and backfat thickness. The OLR1 marker was associated with rump fat thickness and weaning weight (P < 0.05) and the PPARGC1 marker was associated with female yearling weight.

Genetic study of visual scores and hip height at different ages in Nelore cattle

The objective of the present study was to evaluate the genetic variability of visual scores used as selection criteria in Nelore cattle, as well as their associations with yearling hip height (YH) and mature hip height (MH), to determine whether the selection considered would change the hip height of animals. (Co)variance components were obtained by two-trait animal model using Gibbs sampling, including YH or MH in each analysis. Breeding values for YH and MH were used to obtain the response to selection. The posterior mean of the heritability estimates for conformation, finishing precocity (or body condition) and muscling at weaning (WC: 0.21 ± 0.02; WP: 0.21 ± 0.02; and WM: 0.18 ± 0.02 respectively) were lower than those obtained for the same traits at yearling (YC: 0.34 ± 0.02; YP: 0.37 ± 0.02; and YM: 0.38 ± 0.02 respectively). Heritability estimates for YH and MH were 0.28 ± 0.01 and 0.33 ± 0.03 respectively. The results suggested that these traits should respond to selection process, but in different magnitudes. Positive and high genetic correlations were estimated between WC and YH and YC and YH (0.80 ± 0.03 and 0.76 ± 0.03 respectively), and lower values between these scores and MH (0.48 ± 0.09 and 0.36 ± 0.07 respectively). Weak genetic associations were obtained between finishing precocity score and YH and muscling score and YH (ranging from 0.05 ± 0.05 to 0.18 ± 0.06). Moreover, negative and favourable genetic associations between each of WP, WM, YP and YM, and MH (ranging from –0.21 ± 0.07 to –0.12 ± 0.09) were estimated. Correlated responses are expected to be unfavourable for hip height, measured at yearling and at maturity, when animals better genetically evaluated for conformation in relation to finishing precocity and muscling scores are selected. Genetic changes obtained for YH and MH were null (–0.02 cm/year and 0.03 cm/year respectively). It can be explained, in part, by smaller weights in the selection index for conformation rather than finishing precocity and muscling, as applied in the studied herds. However, in herds of beef cattle not evaluated and selected for finishing precocity and muscling scores or that have cows with larger size than acceptable, the adoption of mature hip height as one of the selection criteria can be one alternative for obtaining females with a desirable size.

Genetic relations and indirect response to selection based on indices for scrotal circumference, visual scores and weight gain in beef cattle

Genetic improvement in beef cattle involves evaluation for fertility, growth, carcass and visual scores traits. In general, genetic and economic parameters of these traits are considered for selection index construction. The present study was conducted to establish the magnitude of genetic and phenotypic correlations between visual scores of conformation, precocity and muscling at weaning and at yearling, birth to weaning weight gain, weaning to yearling weight gain and yearling scrotal circumference, and between these traits with selection indices applied in this population. In addition, the expected gains were obtained in such traits by taking under consideration the adopted selection criteria based on indices. Positive and high genetic correlations were estimated between weaning traits (visual scores and weight gain), ranging from 0.70 ± 0.02 to 0.97 ± 0.01. In genetic terms, the same visual scores, but evaluated in two ages (weaning and yearling) showed positive and high associations, with values of 0.90 ± 0.01 (conformation), 0.88 ± 0.01 (precocity) and 0.84 ± 0.02 (muscling). Genetic associations between yearling visual scores with scrotal circumference and weaning to yearling weight gain ranging from 0.36 ± 0.02 to 0.53 ± 0.01. Using the weaning index, are expected genetic gains ranging from 0.26% to 0.70%/year of the phenotypic mean of each weaning trait. Using the yearling index, annual genetic gains estimated for weaning traits (ranging from 0.25% to 0.63% of the phenotypic mean of the trait) were lower than that obtained at yearling (ranging from 0.27% to 0.98% of the phenotypic mean of the trait). Selection for one of traits obtained at weaning should result, by correlated response, in gain for the others. Also, the choice of animals with higher breeding values for weaning visual scores, beyond anticipate the selection process, should lead to favourable changes in these traits at yearling. Selection for better body composition at yearling (higher scores) should provide increase in scrotal circumference and weight gain. The use of higher values of the indices should result in positively correlated response, but in different magnitudes for each trait used in these indices. However, in both weaning and yearling, higher genetic gains to visual scores are expected in the same period, when the selection is based on the values of these indices.