F. Vincent Warren

Width-enhanced Binary Representation for Library Searching of Vapor Phase Infrared Spectra

A method is presented for the representation of vapor phase infrared spectra in a binary format which preserves both width and position information about each peak. Preselection of an optimally width-enhanced library of binary spectra, in advance of any actual library searching, is approached through the application of information theory and Grotchs method for prediction of matching histograms. A direct comparison of library searching results is conducted to complement the preselection efforts. The applicability of a channel-combination approach based on information theory is demonstrated. By this method, adequate library searching is achieved after a 25-fold reduction in the number of channels needed to describe each vapor phase infrared spectrum.

On the need for “Wide-Pores” in the Reversed-Phase Liquid Chromato- Graphic Separation of Proteins

Abstract Pore size information for 13 commercially available reversed-phase columns is determined by application of the GPC technique described by Halasz and Martin. In most cases, the experimental median pore diameter (MPD) is in good agreement with the nominal pore size for the packing material, although the discrepancies are large in a few cases. The measured MPDs are used as a basis for identifying whether pore size is a major contributor to resolution, peak width and sample capacity in the reversed-phase separation of proteins on commercially available columns.

Element-specific derivatization for enhanced detectability by the gas chromatograph-microwave emission detector (GC-MED)

Abstract The applicability or using the gas chromatograph-microwave plasma emission detector (GC-MED) in combination with chemical derivatization based on unique elements for enhanced detectability of selected compounds in complex samples is discussed. A number of potential derivatization schemes are surveyed and results for two specific approaches are presented. In addition to high sensitivity and element selectivity, the possibility of determining empirical formulas by plasma emission will be an advantage unique to this approach.