Fábia de Oliveira Luna

Debris ingestion by the Antillean Manatee (Trichechus manatus manatus)

The Antillean manatee inhabits coastal regions of North and Northeastern Brazil and currently is considered an endangered species in the country. Aiming to gather information for the development of public policies focusing on the conservation of manatees, the National Center for Research and Conservation of Aquatic Mammals of the Chico Mendes Institute for Biodiversity has been rescuing, rehabilitating and releasing these mammals since the 1980s. Over the last 36 years, 40 manatees were released by the CMA/ICMBio and four of them were rescued again due to debris ingestion. Two of these manatees died and the other two were taken back into captivity for a new rehabilitation process. The four mammals had confirmed diagnosis of plastic debris ingestion. These findings demonstrate that the environment where the manatees live after being released had a significant amount of garbage which may hinder the success of the species conservation in Brazil.

Reports of strandings and sightings of bottlenose dolphins (Tursiops truncatus) in northeastern Brazil and Brazilian oceanic islands

This study revises and updates information on bottlenose dolphin Tursiops truncatusstrandings and sightings in northeastern Brazil. A total of 72 strandings were recorded from 1992 to 2010, and 51 sightings were recorded from 1988 to 2010 along the states from Ceara to Bahia, including Fernando de Noronha and Sao Pedro and Sao Paulo archipelagos, Rocas Atoll and Abrolhos Bank. Most strandings were recorded in the states of Pernambuco, Bahia and Rio Grande do Norte. The number of reports was higher during summer, but no statistically significant difference was found between summer and other seasons. The majority of the stranded animals were adults (70.6%). Stranded calves were not observed. Total body length ranged from 138 to 321cm: 138 to 310cm for males; and 168 to 288cm for females. The average adult body length was 281cm (SD = 0.22). For stranding events where sex was determined (n = 29), a highly statistically significant difference was found between the number of males (n = 21) and females (n = 8). A total of six animals (8.3%) showed evidence of fisheries interactions such as net marks or removal of meat, blubber and eyes. Twenty-two sightings were recorded in the northeastern region, mostly in small islands and Rocas Atoll. This finding shows that the bottlenose dolphin is mainly an oceanic species in the region. More efforts are needed to improve our understanding of the populations and conservation status of bottlenose dolphins in this region.

IDENTIFICATION OF BACTERIA IN BLOOD CULTURES FROM CLINICALLY ILL CAPTIVE ANTILLEAN MANATEES (TRICHECHUS MANATUS MANATUS).

Abstract Between September 2001 and March 2013, 62 bacterial cultures (37 aerobic and 25 anaerobic) were performed on 37 blood samples from 23 Antillean manatees (Trichechus manatus manatus) that were kept in captivity at the Brazilian National Center for Research and Conservation of Aquatic Mammals (CMA) in Pernambuco (CMA-PE) and Alagoas (CMA-AL), Brazil. All of the animals sampled exhibited clinical signs at the time of sampling including abscesses (n = 8), debilitation and anorexia (n = 22), and profound lethargy-moribundity (n = 7). The 4 animals with profound lethargy-moribundity died shortly after sampling of unknown causes. Bacteria were isolated from 15/37 (40.5%) and aerobic blood cultures from 13/23 animals (56.5%). None of the anaerobic cultures were positive. Aeromonas caviae, Aeromonas hydrophila, Aeromonas sp., Escherichia coli, Leclercia adecarboxylata, Pantoea agglomerans, Pseudomonas aeruginosa, Pseudomonas stutzeri, Pseudomonas sp., Sphingomonas paucimobilis, coagulase-negative Staphylococcus, and Staphylococcus epidermidis were each found in only one animal; Staphylococcus spp. was found in two; and Vibrio fluvialis in four. Thirteen samples had only one bacteria isolated, one sample had two bacteria, and one sample had three bacteria isolated. Regarding sex, age group, and origin among the manatees examined, 54.5% (6/11) of the females, 58.3% (7/12) of the males, 40% (2/5) of the calves, 66.7% (8/12) of the juveniles, 50% (3/6) of the adults, 55.5% (10/18) at CMA-PE, and 60% (3/5) at CMA-AL were found to be positive for bacterial growth during at least one sampling time. All Antillean manatees were clinically ill. Regarding clinical signs, bacteria were found in 50% (11/22) of blood samples of the animals showing debilitation and anorexia, 1 of 8 (12.5%) of blood samples of the animals showing abscesses, and 3 of 7 (42.9%) of blood samples of the animals showing profound lethargy-moribundity.

SEROPREVALENCE OF TOXOPLASMA GONDII IN CAPTIVE ANTILLEAN MANATEE (TRICHECHUS MANATUS MANATUS) IN BRAZIL.

Abstract Antillean manatees (Trichechus manatus manatus) are aquatic mammals that inhabit marine waters from Central America to the northeastern region of Brazil, and they are an endangered species. Infection with Toxoplasma gondii through intake of water or food contaminated with oocysts has been reported among marine mammals. The present study aimed to evaluate the prevalence of antibodies to T. gondii in West Indian manatees living in captivity in northeastern Brazil. Serum samples from 55 West Indian manatees from three different captive groups were tested for T. gondii antibodies by means of the modified agglutination test using a cutoff of 1:25. The samples were screened at dilutions of 1:25, 1:50, and 1:500, and positive samples were end-titrated using twofold serial dilutions; antibodies were found in six Antillean manatees (10.9%) with titers of 1:50 in three, 1:500 in one, 1:3,200 in one, and 1:51,200 in one manatee. This study is the first report of T. gondii antibodies in captive Antillean manatees in Brazil.

RETROSPECTIVE SURVEY FOR PATHOGENS IN STRANDED MARINE MAMMALS IN NORTHEASTERN BRAZIL: BRUCELLA SPP. INFECTION IN A CLYMENE DOLPHIN (STENELLA CLYMENE)

ABSTRACT:  We surveyed 13 carcasses of marine mammals (12 Trichechus manatus and one Stenella clymene) that had stranded in northeastern Brazil during 1990–2013 for infectious diseases by screening tissues from the collection of the Brazilian National Center of Research and Conservation of Aquatic Mammal, Chico Mendes Institute for Biodiversity Conservation. Brucella spp. and Mycobacterium spp. were investigated by culturing and PCR of tissue samples, whereas Sarcocystidae parasites, Leptospira spp., and Morbillivirus were surveyed for using specific PCR assays. Brucella spp. and Mycobacterium spp. were not isolated through microbiologic culturing, and all animals were negative for detection of Sarcocystidae parasites, Leptospira spp., Mycobacterium spp., and Morbillivirus by PCR assays. All manatees were negative for Brucella spp. infection, but Brucella ceti was detected in the brain tissue of an S. clymene calf by using a PCR assay.

Three Decades of Antillean Manatee (Trichechus manatus manatus) Stranding Along the Brazilian Coast

Alive Antillean manatee stranding is one of the main challenges for the conservation of the species in Brazil. In the present study, data on Antillean manatee stranding recorded by the CMA/ICMBio’s Wild Animals Rehabilitation Center from 1987 to 2015 were analyzed. The CMA and the REMANE’s (Northeastern Aquatic Mammal Stranding Network’s) institutions have rescued on average five Antillean manatees per year. Approximately 56% of the animals were alive when rescued, and 43.7% were found dead. The states of Ceará and Rio Grande do Norte have the highest records of stranding, rescue, and assistance of alive calves. Stranding of alive specimens is more frequent during the austral summer, especially in January, February, and March. In the North region, the main threat to the Antillean manatee population seems to be interaction with fishing activities. Studies on the causes of dependent calf stranding in northeast Brazil are urgently needed to promote the development of public policies for the conservation of the species. Brazil’s manatee rehabilitation centers must undergo improvements to better assist stranded calves, which need special care to survive.