Fabiana Fedatto Bernardon

Morphological, molecular and phylogenetic analyses of Diplotriaena bargusinica Skrjabin, 1917 (Nematoda: Diplotriaenidae)

The nematode Diplotriaena bargusinica is a bird air sac parasite, and its taxonomy is based mainly on morphological and morphometric characteristics. Increasing knowledge of genetic information variability has spurred the use of DNA markers in conjunction with morphological data for inferring phylogenetic relationships in different taxa. Considering the potential of molecular biology in taxonomy, this study presents the morphological and molecular characterization of D. bargusinica, and establishes the phylogenetic position of the nematode in Spirurina. Twenty partial sequences of the 18S region of D. bargusinica rDNA were generated. Phylogenetic trees were obtained through the Maximum Likelihood and Bayesian Inference methods where both had similar topology. The group Diplotriaenoidea is monophyletic and the topologies generated corroborate the phylogenetic studies based on traditional and previously performed molecular taxonomy. This study is the first to generate molecular data associated with the morphology of the species.

Rhinonyssidae in Chrysomus ruficapillus (Vieillot, 1819) (Passeriformes: Icteridae) from Southern Brazil

ABSTRACT: We examined 122 specimens of Chrysomus ruficapillus (Vieillot, 1819) from southern Brazil for nasal mites. The mites, Sternostoma strandtmanni Furman, 1957; Ptilonyssus sairae (Castro, 1948); Ptilonyssus icteridius (Strandtmann and Furman, 1956); and Ptilonyssus sp. were identified. Ptilonyssus sairae was the most prevalent (14.8%), and S. strandtmanni had the highest mean infection intensity (5.53). Co-infections occurred in 15 hosts, and P. sairae and P. icteridius were simultaneously present in 11 birds. This is the first record of S. strandtmanni and P. sairae in C. ruficapillus and the southernmost record of P. icteridius in the Americas.

Helminths of Molothrus bonariensis (Gmelin, 1789) (Passeriformes: Icteridae) from southernmost Brazil

Information about helminths of Molothrus bonariensis (Gmelin, 1789) (Passeriformes: Icteridae) are scarce; in this sense the objective of this paper was to contribute to its knowledge. Five hosts of southern Brazil were examined and the helminths Prosthogonimus ovatus, Tanaisia valida (Digenea), Diplotriaena bargusinica and Synhimantus (Dispharynx) nasuta (Nematoda) were identified. The species T. valida, P. ovatus and S. (D.) nasuta are for the first time registered for the bird in Brazil. Prosthogonimus ovatus, T. valida, D. bargusinica e S. (D.) nasuta are first recorded in M. bonariensis in the southern Brazilian state Rio Grande do Sul.

Rhinonyssidae (Acari: Gamasida) in Ardeidae (Aves: Pelicaniformes) in Brazil

The order of Pelecaniformes is composed of families Ardeidae (Leach, 1820), Thereskiornithidae (Poche, 1904), and Pelecanidae (Rafinesque, 1891) (CRBRO, 2011). The family Ardeidae gathers more than 60 species of herons, egrets and bitterns being one of the largest and more representative with characteristics adapted to wetlands (Sick, 1997). In the State of Rio Grande do Sul, 13 species are recorded Botaurus pinnatus (Wagler, 1829), Ixobrychus involucris (Vieillot, 1823), Nycticorax nycticorax (Linnaeus, 1758), Butorides striata (Linnaeus, 1758), Bubulcus ibis (Linnaeus, 1758), Ardea cocoi Linnaeus, 1766, Egretta thula (Molina, 1782), Syrigma sibilatrix (Temminck, 1824), Ixobrychus exilis (Gmelin, 1789), Tigrisoma lineatum (Boddaert, 1783), Nyctanassa violacea (Linnaeus, 1758), Egretta caerulea (Linnaeus, 1758), and Ardea alba Linnaeus, 1758 (Bencke et al., 2010). Nasal mites are endoparasites that inhabit the respiratory system of birds. They can be found mainly in the membrane lining nasal turbinates. They can also be found in the anterior portion of the nostrils, larynx, trachea, lungs, conjunctival and air sacs (Amaral and Rebouças, 1974a). The Ardeidae are commonly parasitized by species of nasal mites that make up the genus Tinaminyssus Strandtmann and Wharton, 1958 (Rhinonyssidae) (Bregetova, 1950 apud Pence, 1975; Zumpt and Till, 1955 apud Pence, 1972; Fain, 1956 apud Pence, 1972; Pence, 1972; Amaral and Rebouças, 1974b). In this context, the study aimed to report the occurrence of nasal mites from birds of the family Ardeidae in Brazil, contributing to the knowledge of biodiversity of the nasal mites in this country. The species of birds examined were Tigrisoma lineatum, Nycticorax nycticorax, Ixobrychus involucris, Butorides striata, Bubulcus ibis, Ardea alba, Ardea cocoi, Syrigma sibilatrix, and Egretta thula, a total of 30 birds from the municipalities of Pelotas, Rio Grande and Capão do Leão, in the State of Rio Grande do Sul. The animals were donated, after death, by Núcleo de Reabilitação da Fauna Silvestre and Centro de Triagem de Animais Silvestres of Universidade Federal de Pelotas (NURFS-CETAS/UFPel). For collecting the mites, a cut was made from one of the nostrils until reaching the external orifice of the correspondent ear, repeating the process on the opposite side. Next, the nasal turbinates were cut lengthwise and the top of the head was bent backwards until it forms a right angle with the lower part (Fain,1957 apud Amaral and Rebouças, 1974a). After the cavity was washed with water jet through a 150 m sieve, and the resulting content, as well as the nasal cavity, were examined under a stereomicroscope. The mites were fixed in ethanol 70° GL and clarified in lactophenol, and mounted on a slide with a coverslip in Hoyer’s. The identification was based on morphological characteristics according to Pence (1972) and Pence (1975). The parameters evaluated were prevalence and mean intensity according to Bush et al. (1997). The specimens were deposited in the Arthropoda Collection of the Laboratory of Parasitology for Wild Animals of Biology Institute, Federal University of Pelotas (no 444-467). The prevalence was 23% of the total number of birds examined. The species positive for mites were Nycticorax nycticorax, Butorides striata, Bubulcus ibis, Syrigma sibilatrix, and Egretta thula. The mites were identified as belonging to Tinaminyssus (Gamasida: Rhinonyssidae), and two species, Tinaminyssus sp. and Tinaminyssus belopolskii Bregetova, 1950, were found (Table 1). Tinaminyssus belopolskii was described in Russia in Ardea cinerea (Linnaeus, 1758) (Bregetova, 1950 apud Pence, 1975), other Ardeidae reported for this nasal mite were Egretta caerulea (Linnaeus, 1758), Butorides virescens (Linnaeus, 1758), Egretta thula (Molina, 1782), and Egretta tricolor (Statius Müller, PL, 1776), in the State of Louisiana, United States of America (Pence, 1972). Tinaminyssus bubulci Zumpt and Till, 1955 was reported in Bubulcus ibis (Linnaeus, 1758) in Central Africa (Zumpt and Till, 1955 apud Pence, 1972) and in North America (Pence, 1972). Tinaminyssus ixobrychi Fain, 1956 was reported in Africa in Ixobrychus minutus (Linnaeus, 1766) (Fain, 1956 apud Pence, 1972). Tinaminyssus neoixobrychi Pence, 1972 was reported in Ixobrychus exilis (Gmelin, JF, 1789), in North America (Pence, 1972). In Brazil, Tinaminyssus belopolskii was reported for the first time by Amaral and Rebouças (1974b), in the State of São Paulo, parasitizing heron species of the genus Ardea, but their species were not identified by the authors of the paper. The Ardeidae Butorides striata, Bubulcus ibis, Syrigma sibilatrix and Egretta thula are recorded as new hosts for Tinaminyssus belopolskii, in Brazil, and Nycticorax nycticorax is a new host for Tinaminyssus sp.. This report extends the geographic distribution of T.

Checklist of the parasitic nasal mites of birds in Brazil

Abstract The diversity of avian nasal mites has been incompletely studied in South America and most reports have been made on the fauna of Brazil, especially from the 1940s to 1970s. Recently, the development of new research has contributed records of species not previously reported in the country. As a result, a compilation of records of nasal mites (Astigmata: Cytoditidae, Turbinoptidae; Prostigmata: Ereynetidae; Mesostigmata: Rhinonyssidae) of birds in Brazil is presented. One hundred and twenty-four species of nasal mites were recorded parasitizing 168 bird species. The Rhinonyssidae was the family with the highest number of mites reported (108 species). Most nasal mite hosts in Brazil are native birds, however, mites have also been reported parasitizing captive exotic birds, as well as introduced species and domesticated birds.

Lagochilascariasis in cats (Felis catus domesticus) in southern Brazil

Lagochilascariasis, a parasitic disease little known in Brazil, is caused by an ascarid nematode that has a peculiar life cycle, with a predilection site for the cervical region in the final hosts: humans, cats and dogs. We aimed to record the occurrence of Lagochilascaris minor in domestic cats from rural areas in the Municipality of Pelotas, Rio Grande do Sul State, Brazil, with reports of clinical signs and the treatment applied.