Fabiany da Costa Gonçalves
Universidade Federal do Rio Grande do Sul
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Publication
Featured researches published by Fabiany da Costa Gonçalves.
World Journal of Gastroenterology | 2014
Fabiany da Costa Gonçalves; Natália Schneider; Fernanda Otesbelgue Pinto; Fabíola Schons Meyer; Fernanda Visioli; Bianca Pfaffenseller; Patrícia Luciana da Costa Lopez; Eduardo Pandolfi Passos; Elizabeth Obino Cirne-Lima; Luíse Meurer; Ana Helena da Rosa Paz
AIM To investigate the therapeutic effects of mesenchymal stem cells (MSCs) transplanted intraperitoneally and intravenously in a murine model of colitis. METHODS MSCs were isolated from C57BL/6 mouse adipose tissue. MSC cultures were analyzed according to morphology, cellular differentiation potential, and surface molecular markers. Experimental acute colitis was induced in C57BL/6 mice by oral administration of 2% dextran sulfate sodium (DSS) in drinking water ad libitum from days 0 to 7. Colitis mice were treated with 1 × 10(6) MSCs via intraperitoneal or intravenous injection on days 2 and 5. The disease activity index was determined daily based on the following parameters: weight loss, stool consistency and presence of blood in the feces and anus. To compare morphological and functional differences in tissue regeneration between different MSC injection modalities, mice were euthanized on day 8, and their colons were examined for length, weight, and histopathological changes. Inflammatory responses were determined by measuring the levels of different serum cytokines using a CBA Th1/Th2/Th17 kit. Apoptotic rates were evaluated by terminal deoxynucleotidyl transferase-mediated dUDP-biotin nick end labeling assay. RESULTS Intravenous infusion of MSCs was more effective than intraperitoneal treatment (P < 0.001) in reducing the clinical and histopathologic severity of colitis, which includes weight loss, diarrhea and inflammation. An histological evaluation demonstrated decreased colonic inflammation based on reduced crypt loss and reduced infiltration of inflammatory cells. This therapeutic effect was most likely mediated by the down-regulation of pro-inflammatory cytokines [interleukin (IL)-6 and tumor necrosis factor (TNF)]; and by the up-regulation of anti-inflammatory cytokines (IL-10 and IL-4). Intravenous transplantation also induced high levels of IFN that lead to activation of the immunosuppressive activity of the MSCs, which did not occur with intraperitoneal transplantation (P = 0.006). An increase in apoptotic T cells was observed after intravenous, but not intraperitoneal, MSC infusion, suggesting that MSCs can induce apoptosis in resistant T cells in colonic inflammation (P = 0.027). CONCLUSION Our results demonstrate that intravenous treatment is a superior method for reducing colon inflammation compared with intraperitoneal therapy.
PLOS ONE | 2015
Natália Schneider; Fabiany da Costa Gonçalves; Fernanda Otesbelgue Pinto; Patrícia Luciana da Costa Lopez; Anelise Bergmann Araujo; Bianca Pfaffenseller; Eduardo Pandolfi Passos; Elizabeth Obino Cirne-Lima; Luíse Meurer; Marcelo Lazzaron Lamers; Ana Helena da Rosa Paz
Glucocorticoids and immunosuppressive drugs are commonly used to treat inflammatory disorders, such as inflammatory bowel disease (IBD), and despite a few improvements, the remission of IBD is still difficult to maintain. Due to their immunomodulatory properties, mesenchymal stem cells (MSCs) have emerged as regulators of the immune response, and their viability and activation of their migratory properties are essential for successful cell therapy. However, little is known about the effects of immunosuppressant drugs used in IBD treatment on MSC behavior. The aim of this study was to evaluate MSC viability, nuclear morphometry, cell polarity, F-actin and focal adhesion kinase (FAK) distribution, and cell migratory properties in the presence of the immunosuppressive drugs azathioprine (AZA) and dexamethasone (DEX). After an initial characterization, MSCs were treated with DEX (10 μM) or AZA (1 μM) for 24 hrs or 7 days. Neither drug had an effect on cell viability or nuclear morphometry. However, AZA treatment induced a more elongated cell shape, while DEX was associated with a more rounded cell shape (P < 0.05) with a higher presence of ventral actin stress fibers (P < 0.05) and a decrease in protrusion stability. After 7 days of treatment, AZA improved the cell spatial trajectory (ST) and increased the migration speed (24.35%, P < 0.05, n = 4), while DEX impaired ST and migration speed after 24 hrs and 7 days of treatment (-28.69% and -25.37%, respectively; P < 0.05, n = 4). In conclusion, our data suggest that these immunosuppressive drugs each affect MSC morphology and migratory capacity differently, possibly impacting the success of cell therapy.
World Journal of Stem Cells | 2012
Fabiany da Costa Gonçalves; Ana Helena da Rosa Paz; Priscila Schmidt Lora; Eduardo Pandolfi Passos; Elizabeth Obino Cirne-Lima
AIM To investigate the interaction between mesenchymal stem cells (MSCs) and bone grafts using two different cultivation methods: static and dynamic. METHODS MSCs were isolated from rat bone marrow. MSC culture was analyzed according to the morphology, cell differentiation potential, and surface molecular markers. Before cell culture, freeze-dried bone (FDB) was maintained in culture for 3 d in order to verify culture medium pH. MSCs were co-cultured with FDB using two different cultivation methods: static co-culture (two-dimensional) and dynamic co-culture (three-dimensional). After 24 h of cultivation by dynamic or static methods, histological analysis of Cell adhesion on FDB was performed. Cell viability was assessed by the Trypan Blue exclusion method on days 0, 3 and 6 after dynamic or static culture. Adherent cells were detached from FDB surface, stained with Trypan Blue, and quantified to determine whether the cells remained on the graft surface in prolonged non-dynamic culture. Statistical analyses were performed with SPSS and a P < 0.05 was considered significant. RESULTS The results showed a clear potential for adipogenic and osteogenic differentiation of MSC cultures. Rat MSCs were positive for CD44, CD90 and CD29 and negative for CD34, CD45 and CD11bc. FDBs were maintained in culture for 3 d and the results showed there was no significant variation in the culture medium pH with FDB compared to pure medium pH (P > 0.05). In histological analysis, there was a significant difference in the amount of adhered cells on FDB between the two cultivation methods (P < 0.05). The MSCs in the dynamic co-culture method demonstrated greater adhesion on the bone surface than in static co-culture method. On day 0, the cell viability in the dynamic system was significantly higher than in the static system (P < 0.05). There was a statistical difference in cell viability between days 0, 3 and 6 after dynamic culture (P < 0.05). In static culture, cell viability on day 6 was significantly lower than on day 3 and 0 (P < 0.05). CONCLUSION An alternative cultivation method was developed to improve the MSCs adhesion on FDB, demonstrating that dynamic co-culture provides a superior environment over static conditions.
Cytotherapy | 2018
Fabiany da Costa Gonçalves; Michele Aramburu Serafini; Helena Flores Mello; Bianca Pfaffenseller; Anelise Bergmann Araujo; Fernanda Visioli; Ana Helena da Rosa Paz
BACKGROUND AIMS Although mesenchymal stromal cells (MSCs) have shown therapeutic potential in intestinal tissue repair, controversy concerning their short survival and poor biodistribution in recipient tissues still remains. Therefore, we investigated the paracrine role of MSC in three-dimensional culture of colon with experimental colitis. METHODS Colitis was induced in mice by oral administration of dextran sulfate sodium (DSS) for 7 days. Inflammatory responses were assessed on the basis of clinical signs, morphological, and histopathological parameters. On days 2 and 5, colonic explants were removed, and a three-dimensional culture was performed. The structural integrity of the intestinal mucosa was tested by treating the cultures with MSC or conditioned medium (CM) for 24 h, and then the colons were analyzed for histology/immunohistochemistry and interleukin (IL)-6 production. RESULTS Histological analysis demonstrated that both MSC and CM treatment reduced colon damage in organ culture. An increase in cell proliferation (Ki-67 staining) was observed after CM treatment. Additionally, MSC treatment was able to reduce CD3+ cells. The therapeutic effect of MSC and CM was mediated by the downregulation of IL-6. DISCUSSION The intestinal in vitro model has shown to be potentially useful for studying cellular interactions in a three-dimensional cell arrangement. Moreover, our results provide strong evidence that both MSC and CM treatments can alleviate colonic damage in organ culture. Importantly, these results suggest that MSC-secreted factors are able to protect the colon from inflammation caused by DSS-induced colitis independent of cell transplantation.
Acta Scientiae Veterinariae | 2013
Tuane Nerissa Alves Garcez; Ana Helena da Rosa Paz; Alessandra Bileski Magrisso; Helena Flores Mello; Fabiany da Costa Gonçalves; Fabíola Schons Meyer; Emerson Antonio Contesini; Elizabeth Obino Cirne-Lima
Biotechnology Letters | 2017
Fabiany da Costa Gonçalves; Mateus Grings; Natália Schneider Nunes; Fernanda Otesbelgue Pinto; Tuane Nerissa Alves Garcez; Fernanda Visioli; Guilhian Leipnitz; Ana Helena da Rosa Paz
Acta Scientiae Veterinariae | 2013
Fabiany da Costa Gonçalves; Natália Schneider; Helena Flores Mello; Eduardo Pandolfi Passos; Luíse Meurer; Elizabeth Obino Cirne-Lima; Ana Helena da Rosa Paz
Biotechnology Letters | 2017
William Lorenzi; Fabiany da Costa Gonçalves; Natália Schneider; Éverton Franco Silva; Fernanda Visioli; Ana Helena da Rosa Paz; Mauricio Guidi Saueressig
Archive | 2016
Lia Franciê Ribeiro dos Santos Bruschi; Eduardo Cremonese Filippi Chiela; Nayê Balzan Schneider; Anelise Bergmann Araujo; Fabiany da Costa Gonçalves; Ana Helena da Rosa Paz; Patrícia Luciana da Costa Lopez
Archive | 2016
Michele Aramburu Serafini; Fabiany da Costa Gonçalves; Natália Schneider; Fernanda Visioli; Ana Helena da Rosa Paz