Fábio Demolinari de Miranda

Mapeamento de QTL para conteúdos de proteína e óleo em soja

The objective of this study was to detect and map quantitative trait loci (QTL) affecting soybean (Glycine max L. Merr.) protein and oil contents. F2 plants, derived from the cross between the CS3032PTA276 line and the variety UFVS2012, were grown in a greenhouse and provided the leaves for DNA extraction and analysis. Forty-eight polymorphic microsatelite markers (SSR) were evaluated in the F2 population. Evaluation of the phenotype was performed in 207 families from F2:3 progenies, in a complete block design with three replicates, carried out in Vicosa, MG, Brazil, in 2006. Four QTL associated with protein content, in linkage groups D1a, G, A1, and I, and three QTL for oil content in groups A1, I and O were identified. Phenotypic variation for protein and oil explained by QTL ranged from 6.24 to 18.94% and 17.26 to 25.93%, respectively. New QTL associated with protein and oil contents were detected, besides those previously reported in other studies. Other regions may be involved in the genetic control of protein and oil contents in soybean besides those already known.

Transferability and characterization of microsatellite markers in five Bromeliaceae species belonging to the subfamilies Pitcairnioideae and Bromelioideae

Microsatellite markers previously developed for Pitcairnia albiflos Herb. and Pitcairnia geysksii L.B.Sm. were used in cross-amplification tests of five other Bromeliaceae species. Ten (76.9%) out of the 13 evaluated pair of primers had positive results for some of the species tested. The number of polymorphic alleles ranged between two and four in most species. Ho values ranged between zero, in Pitcairnia flammea Lindl. (PaA05), Aechmea ramosa Mart ex Schult & Schult and Billbergia horrida Regel (PaC05), and one in Billbergia euphemiae E. Morren (PaA05, PaA10, PaC05 and PaD07). This study showed that microsatellite markers developed for P. albiflos and P. geysksii effectively amplified the DNA samples of Pitcairnia flammea, Aechmea nudicaulis (L.) Griseb., Aechmea ramosa, Billbergia horrida and Billbergia euphemiae, validating the transferability of these markers to species of the Pitcairnioideae and Bromelioideae subfamilies.