Fábio Lera Orsatti

Plasma hormones, muscle mass and strength in resistance-trained postmenopausal women.

OBJECTIVE To associate changes of body composition, muscle strength (MS) and plasma hormones (PH) in resistance-training protocol in sedentary postmenopausal women (PMW). DESIGN This randomized controlled trial, Brazilian 43 PMW (45-70-year-old) able for physical exercises were selected after they have accomplished medical and ethical criteria. They were assigned in two groups: RT, resistance training (n=22); and CT, not trained control (n=21); with supervision sessions of two to three exercise for large and one exercise for smaller groups in three series of 8-12 rep. (60-80%1RM) for each exercise. The training period lasted 16 weeks and was preceded by low-load exercise (40-50%1RM) adaptation period of 4 weeks (3/(times week)). Body weight, height, body mass index (BMI), and composition (BIA) along with fast-PH (FSH, LH, estradiol, cortisol, IGF-1 and testosterone) were assessed before (M0) and after (M16) the 4 weeks period with the MS (1RM) determined also at 8 weeks (M8). The values were correlated by Persons test and the means compared by Students t-test and ANOVA. RESULTS At baseline both groups were similar in age, time of PMW, body composition, MS and fast-PH. However after 16 weeks, RT presented higher BMI (2.1%), IGF-1 (37.8%) and MM gain (1.8+/-0.8 kg) than CT. MM correlated positively with IGF-1 (r=0.45, p<0.05) and MS progressively increased in all exercise greater in pectoral than legs and upper arms. CONCLUSION Former sedentary postmenopausal women submitted to resistance training gained MM and MS irrespectively of fat mass changes but significantly associated with IGF-1 increase.

Comparison of Predictive Equations for Resting Energy Expenditure in Overweight and Obese Adults

Objective. To compare values from predictive equations of resting energy expenditure (REE) with indirect calorimetry (IC) in overweight and obese adults. Methods. Eighty-two participants aged 30 to 60 years old were retrospectively analyzed. The persons had a body mass index ≥25 kg/m2. REE was estimated by IC and other five equations of the literature (Harris and Benedict, WHO1, WHO2, Owen, Mifflin). Results. All equations had different values when compared to those of IC. The best values were found by Harris and Benedict, WHO1, and WHO2, with high values of intraclass correlation coefficient and low values of mean difference. Furthermore, WHO1 and WHO2 showed lower systematic error and random. Conclusion. No predictive equations had the same values of REE as compared to those of indirect calorimetry, and those which least underestimated REE were the equations of WHO1, WHO2, and Harris and Benedict. The next step would be to validate the new equation proposed.

Low appendicular muscle mass is correlated with femoral neck bone mineral density loss in postmenopausal women

BackgroundAfter menopause, rapid bone mass loss occurs in response to hypoestrogenism. Several studies suggest that muscle mass and bone mineral density (BMD) are positively associated in postmenopausal women. Therefore, it may be assumed that postmenopausal low appendicular muscle mass (aMM) can increase BMD loss in a short period of time.ObjectiveThe purpose of this study was to assess relationship of aMM with femoral neck BMD in postmenopausal women.MethodsProspective, controlled clinical Trial including 64 women aged 45-70 years, who had not had their last menstruation for at least one year. Subjects were divided into two groups: low aMM (n = 32), and normal aMM (n-32). Femoral neck BMD and muscle mass were measured by DXA at baseline and after twelve months. Pairwise and independent t tests were used for data analysis.ResultsBaseline weight, BMI and muscle mass (total and appendicular) significantly differ between groups (p < 0.05). After twelve months, femoral neck BMD was significantly lower in the group with low aMM, whereas no significant difference was observed in the group with normal aMM (p < 0.05).ConclusionIn postmenopausal women, low appendicular muscle mass is associated negatively with femoral neck BMD in a short period of time.

Evaluation of Toll-Like Receptor 2 and 4 RNA Expression and the Cytokine Profile in Postmenopausal Women with Metabolic Syndrome

Objective To evaluate the gene expression of Toll-Like (TLR-2 and TLR-4) receptors and cytokine profile in postmenopausal women with or without metabolic syndrome (MetS). Methods In this cross-sectional study, 311 Brazilian women (age≥45 years and amenorrhea≥12 months) were included. Women showing three or more of the following diagnostic criteria were diagnosed as positive for MetS: waist circumference>88 cm, triglycerides≥150 mg/dL, HDL cholesterol<50 mg/dL, blood pressure≥130/85 mmHg, and fasting glucose≥100 mg/dL. The expression of TLR-2 and TLR-4 in peripheral blood was evaluated by RNA extraction and subsequent real time PCR analysis. The cytokine profile, tumor necrosis factor alpha (TNF-α) and interleukins 1β, 6, and 10, were measured by ELISA. Results The expression of TLR-2 RNA was demonstrated in 32.5% and TLR-4 in 20.6% of the subjects. There was no association between the expression of TLR-2 and TLR-4 and the presence or absence of MetS (P>0.05). A greater production of IL-6 was associated with TLR-2 and TLR-4 expressions and greater production of TNF-α was associated only with TLR-2 expression (P>0.05). Only the lower quartile of IL-10 was associated with the presence of the MetS (P>0.05). Conclusions TLR-2 and TLR-4 expressions were associated with increased pro-inflammatory cytokines, IL-6 and TNF-α, with no association with biomarkers of MetS. The low concentrations of IL-10 may suggest an anti-inflammatory modulation in postmenopausal women with MetS.

MUSCLE MASS GAIN AFTER RESISTANCE TRAINING IS INVERSELY CORRELATED WITH TRUNK ADIPOSITY GAIN IN POSTMENOPAUSAL WOMEN

Abstract Orsatti, FL, Nahas, EAP, Orsatti, CL, de Oliveira, EP, Nahas-Neto, J, da Mota, GR, and Burini, RC. Muscle mass gain after resistance training is inversely correlated with trunk adiposity gain in postmenopausal women. J Strength Cond Res 26(8): 2130–2139, 2012—The aim of this study was to evaluate alterations in trunk adiposity (TA) over 9 months of resistance training (RT) and associate these changes with the hypertrophy of muscle mass (MM) in postmenopausal women (PW). The investigation used a sample that consisted of 22 PW (44–69 years old). The group was subjected to RT (60–80% of 1 repetition maximum) for the total body 3 d·wk−1. Body composition (dual-energy x-ray absorptiometry) and plasma levels of insulin-like growth factor-1 (IGF-1), follicle-stimulating hormone, E2 (Immulite system), and interleukin-6 (IL-6; enzyme-linked immunosorbent assay) were assessed at the beginning and end of the experiment. After RT, only women who acquired up to 5% TA gained MM, whereas women who acquired >5% TA exhibited increased IL-6 and no MM gain (p < 0.05). The &Dgr;MM was negatively associated with time of menopause (r = −0.45, p < 0.05) and positively associated with baseline IGF-1 (r = 0.47, p < 0.05). Only &Dgr;LE (leg extension) was negatively associated with baseline IL-6 (p < 0.05). Trunk adiposity growth (&Dgr;TF, kilograms) was positively correlated with changes in IL-6 (r = 0.68, p < 0.05). The MM gain was negatively correlated with &Dgr;TF (r = −0.63, p < 0.05) and changes in IL-6 (r = −0.73, p < 0.05). After adjusting all of the confounding variables, only baseline IGF-1 (positively) and changes in IL-6 (negatively) influenced MM, and only the increase in TA influenced IL-6. Our study suggests that increased levels of TA during RT increase IL-6 concentrations, which is a significant negative predictor of MM gain in PW.

Effects of resistance training and soy isoflavone on body composition in postmenopausal women

Objective. To investigate the independent and additive effects of resistance training (RT) and soy isoflavone (ISO) on body composition in postmenopausal women (PW). Method. This study used a placebo-controlled, double-blind (soy), randomized (ISO versus placebo) × (RT versus No RT) design. A total of 80 PW, aged 45–70 years, were randomly (71 completed 9-months intervention): RT + ISO (n = 15), No RT + ISO (n = 20), RT + placebo (n = 18), and No RT + placebo (n = 18). ISO received 100 mg a day of isoflavone; and to RT attended supervised resistance training sessions. At baseline and 9-months, fat and muscle mass were estimated by DXA. ANOVA and test t were used. Results. RT groups showed significantly increased muscle strength (35.2%) and muscle mass (1.4%). Exercising attenuated gains in fat trunk and % body fat (P < .05). Significant decreases in muscle mass (−1.8%) and increases in fat mass of the whole-body (1.6%) and trunk (9.7%) was found in no-RT groups (P < .05). In ISO groups, there were no differences in body composition and muscle strength. ISO and RT had no additive effects. Conclusion. In PW: RT improved muscle mass and strength and attenuated gain of fat mass; ISO did not alter body composition and muscle strength; there were no additive effects of RT and ISO.

Association between anthropometric indicators of body fat and metabolic risk markers in post-menopausal women

Objective. to evaluate anthropometric indicators of body fat and their association with metabolic risk markers in postmenopausal women. Methods. A cross-sectional study with 80 Brazilian women (40–70 years) was carried out. Body mass index (BMI = weight/height2), waist circumference (WC) and waist-to-hip ratio (WHR) were obtained for anthropometric evaluation. Trunk fat mass (TFM) was measured by dual-energy X-ray absorptiometry. The following metabolic variables were evaluated: total cholesterol (TC), HDL, LDL, triglycerides (TG), as well as glycemia and insulin to determine insulin resistance (HOMA-IR). Results. Overweight and obesity were observed in 81% of the women. Values of WC >88 cm were observed in 68.5% of the women. On average, TC, LDL and TG levels were above normal levels in 60, 50 and 42.5% of the women, respectively; and HDL was normal in 82.5%. IR was observed in 37.5% of the women. Positive correlations were found between anthropometric indicators and TFM (P < 0.05). WC was most correlated with TFM (r = 0.92), followed by BMI (r = 0.88) and by WHR (r = 0.48; P < 0.05). All anthropometric indicators and TFM showed significant negative correlations with HDL and significant positive correlations with HOMA-IR (P < 0.05). Only WHR was significantly associated with dysglycemia (R2 = 12%), hypertriglyceridemia (R2 = 17%) and decreased HDL (R2 = 27%). WC was significantly associated with HOMA-IR (R2 = 34%). Conclusion. WC and WHR are anthropometric measures that showed strong correlation with TFM and with metabolic risk markers in postmenopausal women.

Indicadores antropométricos como marcadores de risco para anormalidades metabólicas

The purpose of this study was to determine which anthropometric indicator has the greatest bearing on the metabolic abnormalities in participants of a Lifestyle Change Program. It consisted of an exploratory, transversal and analytical survey, which assessed the body mass index (BMI), waist circumference (WC), percentage of body fat (%BF) and of muscle mass (%MM) of 273 adults and elderly subjects (over 40 years of age). Blood samples after an 8-hour diet were obtained to assess total cholesterol, high-density cholesterol, low-density cholesterol, triacylglycerol and glucose. Statistical analyses for differentiation between the groups and determination of associations were conducted. The level of significance was set at p<0.05. When the metabolic abnormalities were assessed as a dependent variable and BMI, WC, %BF, %MM as independent variables, it was seen that WC was the anthropometric indicator that showed the closest association with all metabolic abnormalities (P<0,0001), followed by %MM. The conclusion reached was that WC rather than BMI was the main marker of anthropometric risk for metabolic abnormalities frequently related to obesity. Given the same WC value, overweight and obese individuals had comparable health risks to eutrophic individuals.

Indicadores antropométricos e as doenças crônicas não transmissíveis em mulheres na pós-menopausa da região Sudeste do Brasil

PURPOSE: to evaluate the anthropometric measures as predictors of cardiovascular and metabolic risk in non-transmissible chronic diseases in postmenopausal women. METHODS: a clinical and sectional study enrolling 120 sedentary postmenopausal women (amenorrhea for at least 12 months, age 45 to 70 years was conducted). Exclusion criteria included insulin-dependent diabetes and use of statins or hormone therapy within the preceding six months. Anthropometric indicators included: weight, height, body mass index (BMI=weight/height2), and waist circumference (WC). Metabolic profiles as total cholesterol (TC), HDL, LDL, triglycerides (TG), glycemia, and insulin were measured and the atherogenic index of plasma (AIP) and Homeostasis model assessment-insulin resistance (HOMA-IR) were calculated. One-way analysis of variance (ANOVA) and Odds Ratio (OR) were used in the statistical analysis. RESULTS: subjects were classified on average as overweight and showed central fat distribution. Overweight and obesity were observed in 76% and abdominal obesity in 87.3% of the patients. On average, TC, LDL and TG levels were higher than recommended in 67.8, 55.9 and 45.8% of the women, respectively, and HDL was low in 40.7%. Values of WC >88 cm were observed in 14.8% of women with normal weight, 62.5% overweight and 100% obesity p>0.05). On average, the values of AIP, TG, and HOMA-IR increased significantly along with values of BMI and WC, while decreased HDL (p 88 cm, a risk association was observed with low HDL (OR=5.86; 95%CI=2.31-14.82), with higher TG (OR=2.61; 95%CI=1.18-5.78), with higher AIP (OR=3.42; 95%CI=1.19-9.78) and with IR (OR=3.63; 95%CI=1.27-10.36). There was a risk of low HDL (OR=3.1; 95%CI=1.44-6.85) with increased obesity (BMI>30 kg/m2). CONCLUSIONS: in the postmenopausal women, the simple measure of WC can predict cardiovascular and metabolic risk of non-transmissible chronic diseases.

Predicting Functional Capacity From Measures of Muscle Mass in Postmenopausal Women

Menopause increases body fat and decreases muscle mass and strength, which contribute to sarcopenia. The amount of appendicular muscle mass has been frequently used to diagnose sarcopenia. Different measures of appendicular muscle mass have been proposed. However, no studies have compared the most salient measure (appendicular muscle mass corrected by body fat) of the appendicular muscle mass to physical function in postmenopausal women.