Fabrizio Buldrini

Genetic diversity of the rare and endangered meadow violet (Viola pumila Chaix) at the southern margin of its range

Viola pumila Chaix is a rare violet with a Eurasian-continental distribution. It has undergone a severe population decline during the last decades, and is endangered in Europe. Its southernmost extrazonal occurrence is in northern Italy, with only four populations. Inter-simple sequence repeat markers were used to detect genetic diversity level and distribution in the Italian stand and to compare it to a small sample from Germany. Estimates of genetic diversity revealed retention of genetic variation and weak genetic structure in the Italian populations of V. pumila. Comparable levels of genetic variation were detected in the German population. Principal component and cluster analyses on the whole data-set grouped individuals regardless of their geographical distribution, pointing out that genetic diversity in the V. pumila populations surveyed is homogeneously distributed. Analysis of molecular variance showed that most of the genetic variation is within populations rather than among them. Our results indicate that habitat fragmentation has not induced genetic depauperation and differentiation in the Italian stand of V. pumila; moreover, they provide evidence of past extensive outcrossing and a common evolutionary history of the examined populations. The high diversity detected in this study suggests effective measures for conservation strategies of this rare species.

On the Trail of Date-Plum (Diospyros lotus L.) in Italy and Its First Archaeobotanical Evidence

Diospyros lotus L. is an arboreal species native to the Balkans and Caucasus and ranging to the Far East. In Italy, it has been cultivated for centuries and has reverted to the wild state in some regions. During archaeological excavations carried out in the historical center of Modena (northern Italy), two floral calyxes of D. lotus were discovered in a layer dating from the first century ce. These are the first and only remains of D. lotus found in an archaeological context in Italy thus far. The first historical mention of this species as an arboreal plant in Italy occurs in the 1565 edition of Dioscorides’ Commentarii de Medica materia by Pietro Andrea Mattioli. Our research allowed us to establish that the first three Italian herbaria containing samples of D. lotus, dating to the 1551–1570 period, are the Erbario B of the Biblioteca Angelica of Rome, the Erbario Aldrovandi (Bologna) and the Erbario Cesalpino (Florence). However, archaeobotanical remains reveal that the fruits of this species were known during the Roman period, probably arriving in northern Italy as a luxury food owing to their exceptional flavor.Diospyros lotus L. è una specie arborea nativa dei Balcani e del Caucaso, il cui areale si estende fino all’Estremo Oriente, coltivata in Italia da qualche secolo e inselvatichitasi in talune regioni. In occasione di scavi archeologici condotti nel centro storico di Modena, sono stati scoperti due calici fiorali di D. lotus in uno strato risalente al I sec. d.C.: trattasi dei primi e per ora unici reperti di questa specie rinvenuti in Italia in contesti archeologici. Le prime sicure citazioni storiche della sua presenza sono invece molto piú tarde: chi per primo la descrisse come individui arborei presenti sul territorio italiano è Pietro Andrea Mattioli, nell’edizione del 1565 dei suoi Commentarii ai Discorsi di Dioscoride. Dalle ricerche da noi condotte emerge che pressoché contemporanei sono pure gli erbari italiani che per primi includono campioni di D. lotus: l’Erbario B della Biblioteca Angelica di Roma, l’Erbario Aldrovandi (Bologna) e l’Erbario Cesalpino (Firenze), datati fra il 1551 e il 1570. Le testimonianze archeobotaniche rivelano però che almeno i frutti della pianta erano già noti ai Romani ed erano giunti in Italia settentrionale probabilmente come cibo di lusso per il loro particolare gusto.

“Spigo nardo”: from the Erbario Estense a possible solution for its taxonomical attribution

The spikenard is a plant mentioned since early antiquity, mostly known for its intense and pungent aroma that made it a precious ingredient of many cosmetic recipes for luxury perfumes. The nature of the spikenard has been controversial since the ancient era and, still today, there is the need for a correct attribution of such a name to a currently accepted botanical species. Thanks to a specimen from the Este Herbarium (Erbario Estense), preserved in the Modena State Archives (Italy), we tried to bring our contribution to the debate concerning this topic. We checked all the Italian ancient herbaria in search of samples of spikenard, analysed past literary and medical–pharmaceutical sources from classical antiquity to the XIX century, and searched former and present ethno-botanical uses. Our investigation allowed us to ascertain that the spikenard samples preserved in the Renaissance Italian herbaria belong to Nardostachys jatamansi (D. Don) DC., a herbaceous species belonging to the Valerianaceae, native to alpine regions of the north-western Himalaya, still used in Ayurveda medicine and as a component of cosmetic products.

Diversification of Portulaca oleracea L. complex in the Italian peninsula and adjacent islands

Abstract There is an increasing interest in the taxonomy and distribution of the forms of the Portulaca oleracea complex. The information accruing from specimens collected in the Italian peninsula and surrounding islands (Sicily, Sardinia, Corsica and Malta) is here described. Eleven morphotypes were recorded: ‘P. cypria’, ‘P. granulatostellulata’, ‘P. nitida’, ‘P. oleracea’, ‘P. papillatostellulata’, ‘P. rausii’, ‘P. sardoa’, ‘P. sativa’, ‘P. sicula’, ‘P. trituberculata’, ‘P. zaffranii’ and a still unclear form Portulaca oleracea f. Three occur in almost all the Italian peninsula and adjacent islands; three are scattered in the Italian peninsula and in the adjacent islands; the remnant have a distribution restricted to the islands such as Sicily and Sardinia. The morphotypes can be divided into two main categories: seeds smooth, without ornamentations; seeds with ornamentations. The morphotypes with ornamentations are more widespread than smooth ones, probably because seed ornamentations play an important role in seed dispersal, which is perhaps mainly anthropochorous given that P. oleracea is a synanthropic species that can tolerate mechanical disturbance. There are cases of multiple occurrence, as evidenced by the presence of different morphotypes in some of the sites. Such populations can count up to five morphotypes growing together. Seven morphotypes were here recorded from Malta; they are all hexaploid, even those which in other areas are tetraploid.

Ten years of citizen science data collection of wetland plants in an urban protected area

Abstract Floodplain landscape is the result of man’s presence over a long period of time. This has caused the degradation, or even loss, of several habitats, especially wetlands, which frequently preserve rare biodiversity, even though they are subjected to intense human presence. Human activities can be of value, but can also cause problems in species preservation. The protected area “Ansa e Valli del Mincio” (northern Italy) is an outstanding case study in this respect: it is located in a densely urbanized context, but has great value from a conservation standpoint. This paper presents the results of 10 years of monitoring (2003–2012) of the spatial distribution of two native species (Trapa natans and Nymphaea alba) and the invasive Nelumbo nucifera. Data collected by volunteers from the protected area were processed in GIS and analysed using a number of configurational landscape metrics. Nymphaea alba and N. nucifera became more widespread; T. natans underwent a serious decline, mostly due to the interference of N. nucifera. An oscillating trend of this species is visible until 2008, followed by substantial stability. Furthermore, the potential area of each species was calculated. On the whole, citizen science is a valuable tool to enhance biodiversity knowledge and safeguarding, especially in wetlands that are used for tourism and surrounded by residential areas. As in other contexts, the volunteer contribution was particularly helpful in data collection on a local scale, over a considerable time span.