Fan Jiajia
Dalian Ocean University
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Featured researches published by Fan Jiajia.
Hereditas (beijing) | 2009
Fan Jiajia; Bai JunJie; Li XiaoHui; He XiaoPing; He XiaoYan; Li Shengjie; Ye Xing; Wu LiXin
Forty microsatellite loci were selected to amplify the genomic DNA of cultured stock of largemouth bass (Micropterus salmoides L.) in China. Genotypic differences of these microsatellites loci were analyzed using 2-test between the maximal weight group and minimal weight group. Sixteen microsatellites showing significantly difference (P<0.1) were used in genotyping 121 individuals, and the associations between their genotypes and growth traits were examined. Microsatellite loci of JZL60, JZL67, JZL72, JZL124, MiSaTPW76, MiSaTPW117 and MiSaTPW173 were significantly associated with body weight, body length, and body height (P < 0.05 or P < 0.01). The most favorable genotypes for growth traits were AA at JZL60, BB at JZL67, AC at JZL72, BB at MiSaTPW76 and BC at MiSaTPW117. In addition, a total of 47 alleles for the 16 loci were detected (2 approximately 5 alleles for each locus). The average effective number of alleles (Ae), observed heterozygosity (Ho), expected heterozygosity (He) and mean polymorphic information content (PIC) was 2.938, 0.515, 0.500 and 0.445, respectively, both of which indicated that the population genetic diversity was medium.
Journal of Fisheries of China | 2010
Yu LingYun; Bai JunJie; Fan Jiajia; Li XiaoHui; Ye Xing
Myostatin or GDF-8,a member of the transforming growth factor-β(TGF-β) surperfamily,has been demonstrated to be a negative regulator of myogenesis and inhibited myoblast proliferation during cell cycle and myogenic differentiation in animals.In present research,two SNPs sites(C-1453T and T+33C) were identified from total MSTN gene in larghmouth bass by using PCR-SSCP technique and sequencing;The mutational site of C-1453 was between the regions of E8 box and Octamer(+).the mutation of T+33C was situated in the first extron of the MSTN gene,however the mutation of the first extron has not changed the encoded amino which belonged to the synonymy mutation.The three kinds of genotypes per mutation site were obtained by PCR-RFLP method and the genotype frequency of the mutation was caculated in the population of 128 individuals.A general linear model was used to statistically analyze associations of MSTN gene polymorphisms with major growth traits respectively,and the results indicated that there was no significant difference(P0.05) by all genotypes of single mutation site.Six diplotypes,with the minor allelic frequencies of above 3%,were constructed based on two SNPs in the experiment population.The means of diplotype D2 were over other diplotypes and the means of diplotype D5 were lower other diplotypes in terms of body width,body height,body length,body weight,and snout length therfore associations analysis indicated that there were significant associations between diplotype D2 and D5 in the five growth traits of largemouth bass.The study implies that diplotypes D2 and D5 of MSTN gene could be used as the moleculer genetic marker for selection breeding.
Journal of Fisheries of China | 2009
He XiaoYan; Liu XiaoLin; Bai JunJie; Li Shengjie; Fan Jiajia
Archive | 2013
Yu Lingyun; Bai JunJie; Wang Xiexiang; Fan Jiajia; Quan Yingchun; Li Shengjie; Ma Dongmei; Ye Xing
Archive | 2017
Fan Jiajia; Zhu Bing; Bai JunJie; Jiang Peng; Ma Dongmei; Li Shengjie
Archive | 2017
Li Shengjie; Fan Jiajia; Jiang Peng; Wang Junjie; Hu Jie
Archive | 2017
Ma Dongmei; Quan Yingchun; Fan Jiajia; Hu Jie; Tian Yuanyuan; Jiang Peng; Wang Junjie; Bai JunJie; Li Shengjie; Ye Xing
Archive | 2017
Ma Dongmei; Quan Yingchun; Fan Jiajia; Hu Jie; Tian Yuanyuan; Jiang Peng; Wang Junjie; Bai JunJie; Li Shengjie; Ye Xing
Archive | 2017
Fan Jiajia; Bai JunJie; Jiang Peng; Ma Dongmei; Li Shengjie
Archive | 2017
Ma Dongmei; Bai JunJie; Fan Jiajia; Jiang Peng; Wang Junjie; Yu Lingyun; Quan Yingchun; Li Shengjie