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Dive into the research topics where Farah Benyettou is active.

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Featured researches published by Farah Benyettou.


Journal of Materials Chemistry B | 2015

Synthesis of silver nanoparticles for the dual delivery of doxorubicin and alendronate to cancer cells

Farah Benyettou; Rachid Rezgui; Florent Ravaux; T. Jaber; K. Blumer; Mustapha Jouiad; Laurence Motte; John-Carl Olsen; Carlos Platas-Iglesias; M. Magzoub; Ali Trabolsi

We present the synthesis of a silver nanoparticle (AgNP) based drug-delivery system that achieves the simultaneous intracellular delivery of doxorubicin (Dox) and alendronate (Ald) and improves the anticancer therapeutic indices of both drugs. Water, under microwave irradiation, was used as the sole reducing agent in the size-controlled, bisphosphonate-mediated synthesis of stabilized AgNPs. AgNPs were coated with the bisphosphonate Ald, which templated nanoparticle formation and served as a site for drug attachment. The unreacted primary ammonium group of Ald remained free and was subsequently functionalized with either Rhodamine B (RhB), through amide formation, or Dox, through imine formation. The RhB-conjugated NPs (RhB-Ald@AgNPs) were studied in HeLa cell culture. Experiments involving the selective inhibition of cell membrane receptors were monitored by confocal fluorescence microscopy and established that macropinocytosis and clathrin-mediated endocytosis were the main mechanisms of cellular uptake. The imine linker of the Dox-modified nanoparticles (Dox-Ald@AgNPs) was exploited for acid-mediated intracellular release of Dox. We found that Dox-Ald@AgNPs had significantly greater anti-cancer activity in vitro than either Ald or Dox alone. Ald@AgNPs can accommodate the attachment of other drugs as well as targeting agents and therefore constitute a general platform for drug delivery.


Langmuir | 2016

Redox-Responsive Viologen-Mediated Self-Assembly of CB[7]-Modified Patchy Particles

Farah Benyettou; Xiaolong Zheng; Elizabeth Elacqua; Yu Wang; Parastoo Dalvand; Zouhair Asfari; John-Carl Olsen; Dong Suk Han; Na’il Saleh; Mourad Elhabiri; Marcus Weck; Ali Trabolsi

Sulfonated surface patches of poly(styrene)-based colloidal particles (CPs) were functionalized with cucurbit[7]uril (CB[7]). The macrocycles served as recognition units for diphenyl viologen (DPV(2+)), a rigid bridging ligand. The addition of DPV(2+) to aqueous suspensions of the particles triggered the self-assembly of short linear and branched chainlike structures. The self-assembly mechanism is based on hydrophobic/ion-charge interactions that are established between DPV(2+) and surface-adsorbed CB[7]. DPV(2+) guides the self-assembly of the CPs by forming a ternary DPV(2+)⊂(CB[7])2 complex in which the two CB[7] macrocycles are attached to two different particles. Viologen-driven particle assembly was found to be both directional and reversible. Whereas sodium chloride triggers irreversible particle disassembly, the one-electron reduction of DPV(2+) with sodium dithionite causes disassembly that can be reversed via air oxidation. Thus, this bottom-up synthetic supramolecular approach allowed for the reversible formation and directional alignment of a 2D colloidal material.


Chemistry: A European Journal | 2015

Viologen-Templated Arrays of Cucurbit[7]uril-Modified Iron Oxide Nanoparticles

Farah Benyettou; Katia Nchimi-Nono; Mustapha Jouiad; Yoann Lalatonne; Laurence Motte; John-Carl Olsen; Na’il Saleh; Ali Trabolsi

Magnetic and fluorescent assemblies of iron-oxide nanoparticles (NPs) were constructed by threading a viologen-based ditopic ligand, DPV(2+), into the cavity of cucurbituril (CB[7]) macrocycles adsorbed on the surface of the NPs. Evidence for the formation of 1:2 inclusion complexes that involve DPV(2+) and two CB[7] macrocycles was first obtained in solution by (1)H NMR and emission spectroscopy. DPV(2+) was found to induce self-assembly of nanoparticle arrays (DPV(2+)⊂CB[7]NPs) by bridging CB[7] molecules on different NPs. The resulting viologen-crosslinked iron-oxide nanoparticles exhibited increased saturation magnetization and emission properties. This facile supramolecular approach to NP self-assembly provides a platform for the synthesis of smart and innovative materials that can achieve a high degree of functionality and complexity and that are needed for a wide range of applications.


Journal of Materials Chemistry B | 2013

Toward theranostic nanoparticles: CB[7]-functionalized iron oxide for drug delivery and MRI

Farah Benyettou; Yoann Lalatonne; Fabienne Warmont; Rana Assah; John-Carl Olsen; Mustapha Jouaid; Laurence Motte; Carlos Platas-Iglesias; Ali Trabolsi

Iron oxide (γ-Fe2O3) nanoparticles (NPs) were efficiently coated with the water soluble macrocycle cucurbit[7]uril (CB[7]) by microwave heating. Density functional theory (DFT) calculations support a binding model in which the carbonyl oxygens of CB[7] coordinate directly to surface Fe3+ ions. The modified particles (CB[7]NPs) are stable under a wide pH range (2-12) and have a transverse relaxivity, R2, of 113 s-1 mM-1. Nile red (NR) dye was loaded into the cavities of the surface-adsorbed CB[7]s, and intracellular delivery of the dye to HCT116 cells was observed by confocal laser scanning microscopy. The dye-loaded particles (CB[7]NPs⊃NR) have a R2 of 172 s-1 mM-1. The stability, biocompatibility, and dual purpose functionality (drug delivery and magnetic resonance imaging) of the CB[7]NPs herald the theranostic potential of this system.


Chemistry: A European Journal | 2016

Mesoporous γ-Iron Oxide Nanoparticles for Magnetically Triggered Release of Doxorubicin and Hyperthermia Treatment.

Farah Benyettou; Jaen Alonso Ocadiz Flores; Florent Ravaux; Rachid Rezgui; Mustapha Jouiad; Samer I. Nehme; Rajesh K. Parsapur; John-Carl Olsen; Parasuraman Selvam; Ali Trabolsi

Mesoporous iron-oxide nanoparticles (mNPs) were prepared by using a modified nanocasting approach with mesoporous carbon as a hard template. mNPs were first loaded with doxorubicin (Dox), an anticancer drug, and then coated with the thermosensitive polymer Pluronic F108 to prevent the leakage of Dox molecules from the pores that would otherwise occur under physiological conditions. The Dox-loaded, Pluronic F108-coated system (Dox@F108-mNPs) was stable at room temperature and physiological pH and released its Dox cargo slowly under acidic conditions or in a sudden burst with magnetic heating. No significant toxicity was observed in vitro when Dox@F108-mNPs were incubated with noncancerous cells, a result consistent with the minimal internalization of the particles that occurs with normal cells. On the other hand, the drug-loaded particles significantly reduced the viability of cervical cancer cells (HeLa, IC50 =0.70 μm), wild-type ovarian cancer cells (A2780, IC50 =0.50 μm) and Dox-resistant ovarian cancer cells (A2780/AD, IC50 =0.53 μm). In addition, the treatment of HeLa cells with both Dox@F108-mNPs and subsequent alternating magnetic-field-induced hyperthermia was significantly more effective at reducing cell viability than either Dox or Dox@F108-mNP treatment alone. Thus, Dox@F108-mNPs constitute a novel soft/hard hybrid nanocarrier system that is highly stable under physiological conditions, temperature-responsive, and has chemo- and thermotherapeutic modes of action.


Journal of Bioanalysis & Biomedicine | 2012

Ultra-Small Superparamagnetic Iron Oxide Nanoparticles Made to Order

Farah Benyettou; John-Carl Olsen; Laurence Motte; Ali Trabolsi

A fast, efficient and green microwave-assisted synthesis of ultra-small, superparamagnetic iron oxide nanoparticles is reported. By controlling the temperature and heating mode of a polyol reduction using triethylene glycol (TREG) as a green, high-boiling solvent, ultra-small nanoparticles (2-4 nm) exhibiting robust superparamagnetic behavior were obtained. The sizes of the nanoparticles were determined by TEM and DLS measurements. Using a ligand exchange process, the TREG molecules on the surface of the smallest nanoparticles (2 nm) were successfully replaced with Alendronate, an anti-cancer drug molecule, effectively transforming the nanoparticles into a potential theranostic agent.


RSC Advances | 2017

Selective growth inhibition of cancer cells with doxorubicin-loaded CB[7]-modified iron-oxide nanoparticles

Farah Benyettou; H. Fahs; R. Elkharrag; Rana A. Bilbeisi; B. Asma; Rachid Rezgui; Laurence Motte; M. Magzoub; Jérémy Brandel; John-Carl Olsen; Fabio Piano; Kristin C. Gunsalus; Carlos Platas-Iglesias; Ali Trabolsi

Cucurbit[7]uril-modified iron-oxide nanoparticles (CB[7]NPs) were loaded with doxorubicin hydrochloride (Dox) and tested as a drug delivery system. Dox was found to interact with the carbonyl-rich rims of the CB[7] macrocycles adsorbed on the surface of the nanoparticles. The Dox-loaded nanoparticles (Dox@CB[7]NPs) were stable at room temperature and physiological pH and released their Dox cargo under acidic conditions, in the presence of glutathione, or with heating. Dox@CB[7]NPs reduced the viability of HeLa and three other cancer-derived cell lines in vitro at lower IC50 than free Dox. They were also nontoxic to C. elegans. The sensitivity of HeLa cells to Dox@CB[7]NPs was enhanced when the temperature was elevated by application of an alternating magnetic field. Thus, Dox@CB[7]NPs show promise as agents for the intracellular delivery of Dox to cancer cells, for the selective and controlled release of the drug, and, more generally, as a possible means of combining chemotherapeutic and hyperthermic treatment modalities.


Scientific Reports | 2016

A Chemical Template for Synthesis of Molecular Sheets of Calcium Carbonate

Ina Rianasari; Farah Benyettou; Sudhir Kumar Sharma; Thomas N. Blanton; Serdal Kirmizialtin; Ramesh Jagannathan

Inspired by the discovery of graphene and its unique properties, we focused our research to develop a scheme to create nacre like lamellar structures of molecular sheets of CaCO3 interleaved with an organic material, namely carbon. We developed a facile, chemical template technique, using a formulation of poly(acrylic) acid (PAA) and calcium acetate to create lamellar stacks of single crystal sheets of CaCO3, with a nominal thickness of 17 Å, the same as a unit-cell dimension for calcite (c–axis = 17.062 Å), interleaved with amorphous carbon with a nominal thickness of 8 Å. The strong binding affinity between carboxylate anions and calcium cations in the formulation was used as a molecular template to guide CaCO3 crystallization. Computational modeling of the FTIR spectra showed good agreement with experimental data and confirmed that calcium ions are bridged between polymer chains, resulting in a net-like polymer structure. The process readily lends itself to explore the feasibility of creating molecular sheets of other important inorganic materials and potentially find applications in many fields such as super capacitors and “low k di-electric” systems.


ACS Applied Materials & Interfaces | 2017

Sequential Delivery of Doxorubicin and Zoledronic Acid to Breast Cancer Cells by CB[7]-Modified Iron Oxide Nanoparticles

Farah Benyettou; Marwa Alhashimi; Matthew O’Connor; Renu Pasricha; Jérémy Brandel; Hassan Traboulsi; Javed Mazher; John-Carl Olsen; Ali Trabolsi

Drug-loaded magnetic nanoparticles were synthesized and used for the sequential delivery of the antiresorptive agent zoledronic acid (Zol) and the cytotoxic drug doxorubicin (Dox) to breast cancer cells (MCF-7). Zol was attached to bare iron oxide nanoparticles (IONPs) via phosphonate coordination to form Z-NPs. The unbound imidazole of Zol was then used to complex the organic macrocycle CB[7] to obtain CZ-NPs. Dox was complexed to the CZ-NPs to form the fully loaded particles (DCZ-NPs), which were stable in solution at 37 °C and physiological pH (7.4). Fluorescence spectroscopy established that Dox is released in solution from DCZ-NPs suddenly (i) when the particles are subjected to magnetically induced heating to 42 °C at low pH (5.0) and (ii) in the presence of glutathione (GSH). Mass spectrometry indicated that Zol is released slowly in solution at low pH after Dox release. Magnetic measurements with a magnetic reader revealed that DCZ-NPs are internalized preferentially by MCF-7 cells versus nonmalignant cells (HEK293). Zol and Dox acted synergistically when delivered by the particles. DCZ-NPs caused a decrease in the viability of MCF-7 cells that was greater than the net decrease caused when the drugs were added to the cells individually at concentrations equivalent to those delivered by the particles. MCF-7 cells were treated with DCZ-NPs and subjected to an alternating magnetic field (AMF) which, with the nanoparticles present, raised the temperature of the cells and triggered the intracellular release of Dox, as indicated by fluorescence activated cell sorting (FACS). The cytotoxic effects of the DCZ-NPs on MCF-7 cells were enhanced 10-fold by AMF-induced heating. DCZ-NPs were also able to completely inhibit MCF-7 cell adhesion and invasion in vitro, indicating the potential of the particles to act as antimetastatic agents. Together these results demonstrate that DCZ-NPs warrant development as a system for combined chemo- and thermo-therapeutic treatment of cancer.


Chemistry: A European Journal | 2018

Palladium-Loaded Cucurbit[7]uril-Modified Iron Oxide Nanoparticles for C−C Cross-Coupling Reactions

Farah Benyettou; Laurence Motte; Hassan Traboulsi; Javed Mazher; Renu Pasricha; John-Carl Olsen; Ali Trabolsi; Erwan Guenin

Cucurbit[7]uril modified iron oxide nanoparticles (CB[7]NPs) were loaded with palladium to form nano-catalysts (Pd@CB[7]NPs) that, with microwave heating, catalysed Suzuki-Miyaura, Sonogashira, and Mizoroki-Heck cross-coupling reactions. Reactions were run in environmentally benign 1:1 ethanol/water solvent under convenient aerobic conditions. In a preliminary screening, conversions and yields were uniformly high with turn over frequencies (TOF) ranging from 64 to 7360 h-1 . The nano-catalysts could be recovered with a magnet and reused several times (6 times for Suzuki-Miayura reaction) without loss of activity.

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Ali Trabolsi

New York University Abu Dhabi

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John-Carl Olsen

Indiana University Kokomo

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Mustapha Jouiad

Masdar Institute of Science and Technology

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Rachid Rezgui

New York University Abu Dhabi

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Renu Pasricha

New York University Abu Dhabi

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Florent Ravaux

Masdar Institute of Science and Technology

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Katia Nchimi-Nono

New York University Abu Dhabi

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