Farah Wahida Harun

In vivo Assessment of Antioxidant and Wound Healing Improvement of a New Schiff Base Derived Co (II) Complex in Rats

Co (II) complex (CMLA) was investigated to evaluate the rate of wound healing in rats. Animals were placed into four groups: gum acacia, Intrasite gel, 10 and 20 mg/ml of CMLA. Wounds were made on the dorsal neck area, then treated with Intrasite gel or CMLA; both of these treatments led to faster healing than with gum acacia. Histology of the wounds dressed with CMLA or Intrasite gel displayed a smaller scar width, required less time to heal and showed more collagen staining and fewer inflammatory cells in comparison to wounds dressed with the vehicle. Immunohistochemistry for Hsp70 and TGF-β showed greater staining intensity in the treated groups compared to the vehicle group. Bax staining was less intense in treated groups compared to the vehicle group, suggesting that CMLA and Intrasite gel provoked apoptosis, responsible for the development of granulation tissue into a scar. CD31 protein analysis showed that the treated groups enhanced angiogenesis and increased vascularization compared to the control group. Furthermore, a significant increase in the levels of GPx and SOD and a decrease in MDA were also observed in the treated groups. This results suggest that CMLA is a potentially promising agent for the wounds treatment.

Differentiation of edible fats from selected sources after heating treatments using fourier transform infrared spectroscopy (FTIR) and multivariate analysis

Lard consumption is forbidden for Muslim people and researches have been done to differentiate lard from other fats for Halal authentication. Therefore, this study was conducted to differentiate lard from other edible fats after heating treatments using Fourier transformed infrared spectroscopy (FTIR) and multivariate data analysis techniques. Five samples of fats from different sources; lard, chicken, mutton, tallow and palm based shortening were heated at different temperature (120, 180 and 240 °C) and time (30, 60, 120 and 180 min). The spectra in the form of multivariate data were acquired using FTIR spectroscopy. Principal components analysis (PCA), k-mean cluster analysis (k-mean CA) and linear discriminant analysis (LDA) were used to compare the ability of each technique to differentiate the fats after the heating treatments. It was found that the combination of PCA with k-mean CA was able to differentiate heated fats according to its origin. LDA method was successfully used to classify 80.5 % of samples in its group. Thus, PCA, CA and LDA can be used as multivariate data analysis to differentiate the heated edible fats.Lard consumption is forbidden for Muslim people and researches have been done to differentiate lard from other fats for Halal authentication. Therefore, this study was conducted to differentiate lard from other edible fats after heating treatments using Fourier transformed infrared spectroscopy (FTIR) and multivariate data analysis techniques. Five samples of fats from different sources; lard, chicken, mutton, tallow and palm based shortening were heated at different temperature (120, 180 and 240 °C) and time (30, 60, 120 and 180 min). The spectra in the form of multivariate data were acquired using FTIR spectroscopy. Principal components analysis (PCA), k-mean cluster analysis (k-mean CA) and linear discriminant analysis (LDA) were used to compare the ability of each technique to differentiate the fats after the heating treatments. It was found that the combination of PCA with k-mean CA was able to differentiate heated fats according to its origin. LDA method was successfully used to classify 80.5 % of s...

Synthesis, Characterization and Antibacterial Activityof Schiff Bases Derived from 4-Dimethylaminobenzaldehyde with Some Amino Acids and 4-Aminoantipyrine toward Cu (II), Ni (II), Co (II), Cd (II) and Mn (II) Ions

A new chain of Schiff base complexes made up of Cu (II), Co (II), Cd (II),Ni (II), and Mn (II) ions derivatives were obtained by the condensation of 4-dimethyl-aminobenzaldehyde, and 4-aminoantipyrin and some amino acids. The structures of all synthesized complexes were established on the basis of their elemental analysis (UV, FTIR, and 1 HNMR). The study of X-ray crystallographic shows that the compound (Z)-4-((4(dimethylamino)benzylidene)amino)-1,5-dimethyl-2-phenyl-1,2-dihydro-3H-pyrazol-3-one (L5) crystallized in monoclinic system with space group of C2/c and the unit cell dimensions are a= 17.7916, b= 6.8610, c= 29.7199, α =90.000 β =101.326 γ = 90.000., Z = 8 and V = 3557.20 Å 3 .The antibacterial activity of the complexes are tested against Bacillus subtilis, Bacillus cereus and Pseudomonas aeruginosa.

Synthesis and Characterization 6-Hydroxy 4 Metheyl -5 – Phenyla Zo Coumarins with Divalent Transition Metal Ions

6-hydroxy-4metheyl-5-phenyl azo coumarin have been prepared and characterized by elemental analysis, infrared (IR), proton nuclear magnetic resonance ( 1 H NMR) and Mass spectra. The important infrared (IR) spectral bands corresponding to the active groups in the ligand and the solid complexes under investigation were studied. Also the important fragments in the ligand and the complexes were done using mass spectra and the main peaks were corresponding to the molecular weights of the ligand and complexes. The solid complexes have been synthesized and studied by elemental and as well as by IR, 1 H NMR, mass spectra, molar conductance,. All of the prepared solid complexes behave as non-electrolytes in chloroform.