Faridul Alam

Effect of Rhizobium sp. BARIRGm901 inoculation on nodulation, nitrogen fixation and yield of soybean (Glycine max) genotypes in gray terrace soil.

Soybean plants require high amounts of nitrogen, which are mainly obtained from biological nitrogen fixation. A field experiment was conducted by soybean (Glycine max) genotypes, growing two varieties (Shohag and BARI Soybean6) and two advanced lines (MTD10 and BGM02026) of soybean with or without Rhizobium sp. BARIRGm901 inoculation. Soybean plants of all genotypes inoculated with Rhizobium sp. BARIRGm901 produced greater nodule numbers, nodule weight, shoot and root biomass, and plant height than non-inoculated plants. Similarly, inoculated plants showed enhanced activity of nitrogenase (NA) enzyme, contributing to higher nitrogen fixation and assimilation, compared to non-inoculated soybean plants in both years. Plants inoculated with Rhizobium sp. BARIRGm901 also showed higher pod, stover, and seed yield than non-inoculated plants. Therefore, Rhizobium sp. BARIRGm901 established an effective symbiotic relationship with a range of soybean genotypes and thus increased the nodulation, growth, and yield of soybean grown in gray terrace soils in Bangladesh. Graphical Abstract Rhizobium sp. BARIRGm901 isolation, seedling infectivity test, nitrogenase enzyme assay, and seed yield in soybean genotypes in gray terrace soil.

Effect of molybdenum on nodulation, plant yield and nitrogen uptake in hairy vetch (Vicia villosa Roth)

Abstract Hairy vetch (Vicia villosa Roth) is a leguminous cover crop that is generally used as a green manure to sustain soil health in arable land. Molybdenum (Mo) acts as a cofactor for the nitrogenase (NA) and nitrate reductase (NR) enzymes, which are important for nitrogen (N) fixation, nitrate reduction and N transport in plants. In this study, we applied various doses of Mo to soil to evaluate their efficacy on nodulation, nodule characteristics and biomass production of hairy vetch. Mo application increased the number and size of nodules and NA and NR enzyme activity in hairy vetch. This increase in enzyme activity increased N assimilation and led to higher biomass yield. Plants grown in soil that received 0.5 mg Mo kg−1 showed optimal physical and biochemical properties in nodules, and these properties may explain the increased N fixation in hairy vetch. Higher Mo doses (1.0 mg kg−1) led to the deterioration of nodule structure and, hence, reduced enzymatic activity in plants. The 16S rRNA gene sequencing and cluster analysis showed that the bacterial isolates found in the nodules of hairy vetch roots belonged to the Rhizobiaceae family and shared high sequence similarity with Rhizobium leguminosarum and Agrobacterium tumefaciens. Application of 0.63 mg Mo kg−1 to soil was the optimum dose to maximize the biomass yield of hairy vetch.

Evaluation of Nitrogen and Phosphorus Balance in Green Manure-Rice Cropping Systems without Incorporation of Green Manure Crops

BACKGROUND: The nutrient balance in Korea during 1985-2006 had continually increased and maintained the highest levels among OECD countries. The use of green manure crops such as barley and hairy vetch is common practice for reducing chemical fertilizer application and maintaining soil fertility. However, green manure crops can also be used as a livestock feeding material which may reduce nutrient balance in the national scale. We calculated nitrogen and phosphate balance under green manure-rice cultivating system where all green manure was removed and used for feeding livestock. METHODS AND RESULTS: The barley and hairy vetch grown in pure stands or in mixtures with different sowing rates were tested for rice cultivation without chemical fertilization. The conventional fertilization (NPK) for rice cultivation was selected to compare nutrient balance with green manure-rice cultivation. Nitrogen and phosphate balance were calculated according to the surface balance method of the PARCOM guidelines. Total aboveground biomass of mixture (barley and hairy vetch) was higher compared to that of pure barley or hairy vetch. Among the mixture with barley and hairy vetch, the highest aboveground biomass was observed in B75H25 (barley 75%+hairy vetch 25%). The nitrogen and phosphate balance in the B75H25 mixture was-104 kg N/ha and-50.3 kg P/ha, respectively. CONCLUSION(S): The barley and hairy vetch mixture proved to be a very effective strategy for biomass production of green manure. The amount of nitrogen and phosphate fertilizer to be applied is estimated to be 104 kg N/ha and 50.3 kg P/ha in order to maintain soil fertility if all green manure and rice straw were removed from rice field for livestock feeding.

Green Manuring Effect of Pure and Mixed Barley-Hairy Vetch on Rice Production

BACKGROUND: The mixtures of legumes and non legumes can be an efficient tool to combine the benefit of the single species in the cover crop practice. However, there is a lack of information on how the species proportion may affect N accumulation and how this can influence the nitrogen use of subsequent rice production. METHODS AND RESULTS: In this study barley and hairy vetch was selected as a green manure. The pure stands or mixtures with different seeding ratios was tested on green manure N accumulation and its following rice cultivation. Total aboveground biomass and N accumulation of mixture were higher compared to that of pure barley and hairy vetch. Among the mixtures, the highest aboveground biomass (8.07 Mg/ha) and N accumulation (131 kg/ha) was observed in B75H25 (barley 75% + hairy vetch 25%). The N accumulation of the mixture ranged from 99 kg/ha to 131 kg/ha which was much higher than amount of recommended (90 kg/ha) for rice. All mixture (barley 75%+hairy vetch 25%, barley 50%+hairy vetch 50%, barley 25%+hairy vetch 50%) produced 7-8% more rice yield than the conventional cultivation (NPK). The rice yield of in barley monocrop was 4% less than that of NPK. COLCLUSION(S): Adopting mixtures of barley and hairy vetch could be efficient strategy for rice production as an alternative of nitrogen fertilizer.

GENOTYPIC EVALUATION, HETEROTIC POTENTIAL AND COMBINING ABILITY OF YIELD AND FRUIT QUALITY ATTRIBUTING TRAITS IN TOMATO (Solanum lycopersicum L.).

1. Plant Breeding Division, Bangladesh Agricultural Research Institute, Gazipur 1701, Bangladesh. 2. Department of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur 1706, Bangladesh. 3. Department of Horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur 1706, Bangladesh. 4. Soil Science Division, Bangladesh Agricultural Research Institute, Gazipur 1701, Bangladesh. ...................................................................................................................... Manuscript Info Abstract ......................... ........................................................................ Manuscript History

Evaluation of Phosphorus Balance in Green Manure-Rice Cropping Systems with Different Incorporation Rate of Green Manure Crops

BACKGROUND: In Korea, green manure has been cultivated for reducing chemical fertilizer application, maintaining soil fertility, and feeding livestock in winter season. We evaluated the phosphate balance under green manure-rice cultivating system with different removal rates of green manure for maintaining soil fertility. METHODS AND RESULTS: The barley and hairy vetch mixture was selected as the green manure in this study. The barley and hairy vetch was sowed at a rate of 135 and 23 kg/ha, respectively, without fertilizer application. Total aboveground biomass was 12000 (barley: 5400 kg/ha, hairy vetch: 6600 kg/ha) kg/ha, and these green manure were incorporated with different input rates before rice planting. The input rates of green manure in this study were 0 (NPK+0%), 25 (NPK+25%), 50 (NPK+50%), 75 (NPK+75%) and 100 % (NPK+100) and the standard fertilization (NPK) without green manure cultivation. All treatments were applied with standard fertilizer (N-P-K: 90-19.6-48.3 kg/ha) before rice planting. The highest rice yield was observed in NPK+50% which was 20% higher compared with NPK. The phosphate balance with different incorporation rates of green manure was-104.0,-76.8,-52.9,-27.4, and 6.0 kg/ha for NPK+0%, NPK+25%, NPK+50%, NPK+75%, and NPK+100%, respectively. CONCLUSION(S): The use of green manure for livestock feeding in green manure-rice cropping systems could remove a huge amount of phosphate. This cropping system strongly requires phosphate application before green manure seeding for maintaining soil fertility.

Effect of Application Rate of Hairy Vetch on Ammonia Emission from Paddy Soil

BACKGROUND: Hairy ventch (Vicia villosa) is a good green manure for supplying nitrogen in arable soil. Ammonia emission from rice fields can occur, and the degree of this emission can be great. However, quantitative information of ammonia emission from paddy soil using green manure is required to obtain emission factors for rice cropping in Korea. METHODS AND RESULTS: Ammonia emission from flooding soil with different application rate of hairy vetch was measured using the closed chamber method. For this study, hairy vetch was applied at rates of 0 (control), 500 (H500), 1000 (H1000), 2000 (H2000), and 3000 (H3000) kg/ha (fresh matter basis). This experiment was conducted for 54 days under flooding condition. The total NH3 emission throughout the experiment period was 0.32, 0.54, 1.20, 4.20, and 6.20 kg/ha for control, H500, H1000, H2000, and H3000, respectively. The ratio of NH3 emission to applied nitrogen by hairy vetch for each treatment was 0.7, 1.4, 3.2, and 3.2% for H500, H1000, H2000, and H3000, respectively. CONCLUSION(S): A very small amount of ammonia emission was recorded in the present study. Therefore, the use of hairy vetch in paddy field instead of chemical fertilizer can reduce ammonia emissions.