Faruk Aral

Effect of melatonin on epididymal sperm quality after testicular ischemia/reperfusion in rats

OBJECTIVE To determine the effect of melatonin, a pineal secretory product that prevents testicular ischemia/reperfusion (IR) injury through its antioxidative properties, on epididymal sperm quality in a rat testicular IR injury model. DESIGN Experimental study. SETTING University pharmacology laboratory. ANIMAL(S) Fifty-six 8-week-old male Wistar albino rats. INTERVENTION(S) Left testicular artery and vein occluded for 1 hour; before the bilateral orchiectomy, the organ was allowed to reperfuse 30 days. Melatonin (10 mg/kg IP) or vehicle (1% ethanol in saline) was administrated for 10 minutes before reperfusion and for 1 hour after reperfusion. MAIN OUTCOME MEASURE(S) After 24 hours of reperfusion, the rats were decapitated, and the testicular tissue samples were obtained for histologic examination. In addition, after 30 days of reperfusion, the epididymal sperm concentration, motility, and abnormal sperm rates were determined in the sperm collected from the epididymis. RESULT(S) A statistically significant decrease in sperm concentration resulted from IR as well as an increase in sperm abnormalities, but the sperm motility did not change. Melatonin treatment did not prevent the IR-induced reduction in sperm concentration. However, melatonin treatment statistically significantly decreased the sperm abnormalities when compared with the IR injured samples. CONCLUSION(S) Melatonin may improve sperm morphology for a protective effect in IR-induced testicular injury.

The Effect Of Grape Molasses As An Extender On Motility, Viability And Fertility In Rainbow Trout (Oncorhynchus Mykiss W., 1792) Sperm

Bu calismada, gokkusagi alabaliklarinin ( Oncorhynchus mykiss ) dondurulan spermasina % 5, 7 ve 10 oraninda katilan pekmezin sperma motilitesi, hareketliligi ve dollenme orani uzerine etkisi incelendi. Alti adet erkek balik spermasi % 7 dimetil sulfoksit ile birlikte % 5, 7 ve 10 oraninda pekmez iceren sulandirici ile sulandirildi. Spermalar 0.25 ml payetlerde, sivi azot buharinda dondurularak 2 hafta boyunca sivi azot icerisinde muhafaza edildi. Spermatozoa motilitesi uzerine pekmez etkisi acisindan, %10 oraninda uzum pekmezinin (P 0.05) dollenme orani uzerine pekmezin tum oranlarinda onemli bir fark bulunamadigi saptandi. Ancak, % 10 oraninda kullanilan pekmez ile dondurulan spermalardan elde edilen dollenme orani glikoz sulandiricisi icin elde edilen en yuksek degerden daha iyi oldu (96.4%). Calisma sonuclarina gore, % 10 oraninda pekmezin gokkusagi alabaliklarinin spermalarinin dondurulmasinda kullanilabilecegi kanaatine varildi.