Fatih Özan

Prevalence of commonly found pathoses associated with mandibular impacted third molars based on panoramic radiographs in Turkish population

OBJECTIVE The objective was to determine the association between commonly found pathologic conditions and angulations and impaction depths of lower third molar teeth. STUDY DESIGN In this retrospective study 1,914 panoramic radiographs with 3,050 impacted lower third molars (ILTMs) were examined. RESULTS The problems and the prevalence evaluated were caries on mandibular second molar (12.6%), caries on ILTM (5.3%), bone loss at the distal aspect of ILTM (9.7%), and periodontal tissue damage on adjacent tooth (8.9%). A total of 26.5% of all the cases were affected by any one of the 4 pathologic changes. It was determined that horizontal and mesioangular ILTMs had high risk of caries development on second or third molar; in contrast, vertical and distoangular ILTMs had low risk. Distoangular and vertical ILTMs had high risk of development of bone loss at the distal aspect. Mesioangular and horizontal ILTMs had a high risk for periodontal tissue damage on an adjacent tooth. When evaluating impaction depth of the ILTM, class A had a higher risk of pathology than class B or C. CONCLUSIONS Horizontal and mesioangular impactions were found with more pathologic situations; especially in class A impaction depth. Angulation and impaction depth of the ILTM should be taken into consideration when making a decision whether to extract an ILTM or not.

In vitro evaluation of the amoebicidal activity of garlic (Allium sativum) extract on Acanthamoeba castellanii and its cytotoxic potential on corneal cells.

Free-living protozoa of the genus Acanthamoeba can cause one of the most severe, potentially sight-threatening infections of the eye, the so-called A. keratitis. A. keratitis is difficult to treat because, under adverse conditions, the amoeba encyst and medical therapy is often less effective against cysts than against trophozoites. The aim of this study was to investigate evaluate the in vitro effect of the nonpolar subfraction of the methanol extract of garlic (Allium sativum) on the growth of A. castellanii trophozoites and cysts and also its cytotoxicity on corneal cells in vitro. Extract was evaluated for its amoebicidal activity, using an inverted light microscope. The effect of the nonpolar extract with the concentrations, ranging from 0.78 to 62.5 mg/mL on the proliferation of A. castellanii trophozoites and cysts, were examined in vitro. For the determination of cytotoxicity of the extract on corneal cells, agar diffusion tests were performed. The present study demonstrates the in vitro effectiveness of the garlic against the A. castellanii growth curve. Evaluations revealed that garlic inhibits trophozoite growth in dose- and time-dependent ways. In the case of the cyctotoxic acitivities, it showed no cytotoxicity for the cornea cells in the concentration of 3.90 mg/mL. These findings indicate that nonpolar subfraction of the methanol extracts of garlic has amoebicidal, as well as its cysticidal, properties on Acanthamoeba trophozoites and cysts. Garlic alone, and in combination with other amoebicidal agents, may be used in clinical practices after further investigations.

Systemic propolis stimulates new bone formation at the expanded suture A histomorphometric study

OBJECTIVE To investigate the effects of systemically given propolis on the expanded premaxillary suture in a rat study model. MATERIALS AND METHODS The 24 rats were randomly divided into three groups-only expansion (OE), expansion plus propolis (PRO), and nonexpansion (control) groups. After the 5-day expansion period was completed, the OE and PRO groups underwent 12 days of mechanical retention. At the end of this period, the animals were euthanatized and their pre-maxillae were dissected and fixed. Histomorphometric examination was performed to determine the number of osteoclasts, osteoblasts, and capillaries as well as the intensity of inflammatory cells and amount of new bone formation. RESULTS Statistical analysis showed that the intensities of inflammatory cells, number of osteoblasts, and amount of new bone formation were greater in the PRO group than in the other groups. The PRO group also had more osteoclasts and new capillaries. CONCLUSION Systemic use of propolis may hasten new bone formation at the expanded suture in rats.

Cytotoxicity Analysis of Strontium Ranelate on Cultured Human Periodontal Ligament Fibroblasts: A Preliminary Report

BACKGROUND/PURPOSE The aim of this study was to analyze the cytotoxicity of strontium ranelate (SR) on human periodontal ligament fibroblasts (PDL cells) in vitro. METHODS PDL cells were obtained from healthy human third molars and cultured in Dulbeccos Modified Eagles Medium. The experimental groups were: G1, cultures treated with fresh medium (control); and G2, G3, G4 and G5: treated with SR at 20, 10, 5 and 2.5 mg/mL, respectively. The experimental times were 1, 6, 12 and 24 hours (short-term) for viability, and 2, 4, 6 and 8 days (long-term) for cell survival. The cells were counted using a hemocytometer. Data were then analyzed by one-way ANOVA and Tukeys tests (p < 0.05). RESULTS Cultures treated with the highest SR concentrations (G2 and G3) had significantly lower cell viability and cell numbers (p < 0.05) than those in G1, G4 and G5. SR at 2.5 mg/mL was non-cytotoxic to PDL cells. CONCLUSION SR was non-toxic at appropriate concentrations. Preclinical tests are needed to further assess its safety and effectiveness for tooth resorption prior to clinical use.

Propolis accelerates the consolidation phase in distraction osteogenesis.

Abstract We evaluated the effect of propolis on new bone formation after distraction osteogenesis (DO). This study examined 3 groups: control group, P100, and P200. Rabbits underwent DO of the left mandible after an osteotomy between the first molar and the mental foramen. Bone mineral content and bone mineral density were evaluated using dual-energy x-ray absorption 1 and 4 weeks after the procedure. The volume of connective tissue and new bone and the number of capillaries were measured using stereologic analysis after the subjects were killed. Dual-energy x-ray absorption showed that the bone mineral content and bone mineral density were higher in the groups treated with propolis by week 4, and these parameters were higher in the P200 group. Stereologic analysis showed no significant differences in connective tissue volume and number of capillaries among the groups. New bone volume was lowest in the P200 group. We concluded that propolis accelerates bone formation and may shorten the consolidation phase with DO.

Effect of Royal Jelly on new bone formation in rapid maxillary expansion in rats

Background The aim of this study was to evaluate the effects of long and short term systemic usage of royal jelly on bone formation in the expanded maxillary suture in a rat model. Material and Methods Twenty eight Wistar albino rats were randomly divided into 4 equal groups: Control (C); Only Expansion (OE), Royal Jelly (RJ) group, Royal Jelly was given to rats by oral gavage only during the expansion and retention period; Royal Jelly plus Nursery (RJN) group, Royal Jelly was given to rats by oral gavage during their nursery phase of 40 days and during the retention period. After the 5 day expansion period was completed, the rats underwent 12 days of mechanical retention. All rats were sacrificed in same time. Histological examination was performed to determine the number of osteoclasts, number of osteoblasts, number of capillaries, inflammatory cell infiltration, and new bone formation. Results New bone formation, number of osteoclasts, number of osteoblasts, and the number of capillaries in the expanded maxillary sutures were higher in the RJ and RJN groups than in the other groups. Statistical analysis also demonstrated that new bone formation and the number of osteoblasts was also highest in the RJN group. Conclusions The systemic administration of Royal Jelly in conjunction with rapid maxillary expansion may increase the quality of regenerated bone. Key words:Bone formation, rapid maxillary expansion, Royal jelly.

Intraosseous epidermoid cyst associated with impacted mandibular wisdom teeth: an uncommon entity.

Epidermoid and dermoid cysts of the jaws are seen rarely. The formation theories of the intraosseous epidermoid cyst (IEC) are not clear. The radiographic appearance is similar with unilocular cysts. Surgical enucleation is the suggested treatment method for epidermoid cysts. This case report presents bilateral mandibular intraosseous epidermoid cysts with impacted wisdom teeth which is the first documented case in the literature.

Guided Bone Regeneration Technique Using Hyaluronic Acid in Oral Implantology

Guided bone regeneration is a term used to describe the use of the barrier membranes to enhance complete osteogenesis by preventing the rapid ingrowth of fibroblasts into a bony defect and promoting the migration of osteogenic cells from adjacent bony edges or bone marrow into the defect in an unimpeded fashion. Hyaluronic acid (HA) is a glycosaminoglycan of the general formula (C14H22NO11)n and is an essential component of the extracellular matrix in connective tissue, which is found in abun‐ dance in the alveolar environment. The most important function of HA is its involve‐ ment in tissue healing and repair. It has been shown that HA stimulates cell proliferation, migration and angiogenesis, re-epithelialization and proliferation of basal keratinocytes and reduces collagen and scar tissue formation. This text presents our clinical experiences and outcomes following HA applications in various implant surgery procedures. According to our clinical outcomes, HA is a highly promising material for improving therapeutic outcomes for oral implantology.

Association of Toll-like receptors 2, 3, and 4 genes polymorphisms with periapical pathosis risk

Background The aim of this study was to investigate the role of gene variations of Toll-like receptors (TLR) 2, 3, and 4 on genetic susceptibility to periapical pathosis. Material and Methods One hundred patients were included in the study and divided into two groups as follows; Control Group (n=50) that have root canal treatment and no periapical lesion, Patient Group (n=50) that have root canal treatment and periapical lesion. TLR2 Arg753Gln, TLR3 (c.1377C/T) and TLR4 Asp299Gly and Thr399Ile polymorphisms were genotyped by using PCR-RFLP. Genotypical analysis of control and patient groups were investigated to disclose whether there is any association between periapical lesions and gene variations. Results There are no significant statistical differences between control and patient groups according to TLR 2 and 4 gene sequence. On the contrary, CC allele detected 74% for TLR 3 in patient group, and this difference was found to be statistically significant (p < 0.005). Conclusions According to these results, it can be suggested that patients with Toll-like receptor 3 gene polymorphisms could be susceptible to periapical pathosis. Key words:Toll-like receptors, periapical pathosis, endodontics.

Oral mikroorganizmalara karşı propolisin antimikrobiyal etkinliği

Cesitli amaclarla halk hekimliginde binlerce yildir dogal urunler kullanilmaktadir. Bu urunlerin arasinda, patojenik mikroorganizmalar uzerine antimikrobiyal aktivitesi cok iyi olan propolis, son zamanlarda cok fazla ilgi cekmektedir. Propolis icerigi, arinin gezdigi alanlardaki floraya ve toplandigi zamana gore oldukca farklilik ve zenginlik gostermektedir. Kimyasal bilesimlerindeki farkliliklara ragmen, tum propolis urunleri belirgin bicimde antimikrobiyal etkinlik gostermektedir. Bunlar, farmakolojik olarak aktif molekuller flavonoidler, fenolik asitler ve esterler olarak sayilabilir. Son zamanlarda yapilan calismalardan elde edilen verilere gore propolis, agizdaki patojenik mikroorganizmalar uzerine belirgin inhibisyon gostermektedir. Antimikrobiyal ajanlara karsi mikroorganizmalarin direnc gelistirmeleri, gelistirdikleri direnc mekanizmalarini bakteriler arasi iliski ile birbirlerine aktarmalari ve diger yan etkileri de goz onunde bulunduruldugunda agiz boslugu hastaliklarinin tedavisinde yeni tedavi yontemleri oldukca onem arz etmektedir.