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Feng Juan

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Featured researches published by Feng Juan.

Archive | 2018

哈维弧菌 rbsB 基因的克隆与表达

贝蕾; Bei Lei; 苏友禄; Su Youlu; 赵超; Zhao Chao; 徐力文; Xu Liwen; 刘广锋; Liu Guangfeng; 王雨; Wang Yu; 郭志勋; Guo Zhixun; 冯娟; Feng Juan

哈维弧菌( Vibrio harveyi )是海水鱼养殖中一种重要的病原菌。该研究根据已知 rbsB (核糖体结合蛋白)基因序列设计引物，扩增得到哈维弧菌354 ( V.harveyi 354) rbsB 基因的全序列(GenBank序列号MF797015)，预测该序列可编码292个氨基酸，分子量为30.7 kD，理论等电点为5.05，亲水性系数为0.043，为疏水性蛋白。根据RbsB氨基酸构建的系统进化树可以发现哈维弧菌RbsB蛋白和欧文弧菌( V.owensii CAIM 1854)的关系最近。构建了pGEX-4t-1- rbsB 重组质粒并转化至大肠埃希菌BL21 (DE3)中，得到的重组蛋白的相对分子量约59 kD，在37 ℃、IPTG浓度为0.6 mmol·L –1 诱导8 h时表达量最高。

Journal of fishery sciences of China | 2005

Changes of immune factors in serum of Halliotis diversicolor Reeve infected by virus

Wang Jiangyong; Guo Zhixun; Feng Juan; Pan Jin-pei; Chen Bi-sheng

Archive | 2013

Primer group, kit and detection method for detecting reovirus and bicistronic virus of scylla serrata

Guo Zhixun; Feng Juan; Su Youlu; Zhang Di; Wu Kaichang

Archive | 2013

Guava leaf and new application of extract thereof

Guo Zhixun; Li Zhuojia; Feng Juan; Wang Ruixuan

South China Fisheries Science | 2012

Identification and analysis of resistant plasmid of pathogenic bacteria Vibrio harveyi isolated from Haliotis diversicolor

Wang Ruixuan; Geng Yujing; Feng Juan; Wang Jiangyong

Archive | 2015

Real-time fluorescence quantification RT-PCR (Reverse Transcription-Polymerase Chain Reaction) method, primer group, probe and kit for detecting green crab dicistroviridae-1

Guo Zhixun; Zhang Di; Ma Hongling; Feng Juan; Su Youlu

South China Fisheries Science | 2010

Preparation of polyclonal antibody for major capsid protein (MCP) of RGNNV.

Su Youlu; Guo Zhixun; Feng Juan; Yan Yunfeng; Yu Dahui

Archive | 2017

Detection primer set of Streptococcus agalactiae, detection kit and multiplex PCR detection method

Su Youlu; Liu Chan; Feng Juan; Guo Zhixun; Wang Jiangyong

Archive | 2016

Detection primer group, detection kit, and detection method used for simultaneous detection of three pathogenic bacteria of marine fishes

Su Youlu; Feng Juan; Sun Xiuxiu; Guo Zhixun; Ma Hongling

Nanfang Shuichan Kexue | 2016

2012年~2014年の南シナ海の海水養殖魚類の病原菌哈維弧哈維弧の薬剤耐性分析【JST・京大機械翻訳】

Jiang Kui; Xu Liwen; Su Youlu; Wang Yu; Guo Zhixun; Xu Haidong; Gao Fang; Feng Juan

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Su Youlu

South China Agricultural University

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Wang Jiangyong

Chinese Academy of Fishery Sciences

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Xu Haidong

Shanghai Ocean University

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Wang Ruixuan

Shanghai Ocean University

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Yu Dahui

Chinese Academy of Fishery Sciences

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