Fengqing Gao
Soochow University (Suzhou)
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Featured researches published by Fengqing Gao.
Biological Trace Element Research | 2006
Fan Yang; Fashui Hong; Wenjuan You; Chao Liu; Fengqing Gao; Cheng Wu; Ping Yang
Previous research showed that nano-TiO2 could significantly promote photosynthesis and greatly improve growth of spinach, but, we also speculated that an increase of spinach growth by nano-TiO2 treatment might be closely related to the change of nitrogen metabolism. The effects of nano-anatase TiO2 on the nitrogen metabolism of growing spinach were studied by treating them with nano-anatase TiO2. The results showed that, nano-anatase TiO2 treatment could obviously increase the activities of nitrate reductase, glutamate dehydrogenase, glutamine synthase, and glutamic-pyruvic transaminase during the growing stage. Nano-anatase TiO2 treatment could also promote spinach to absorb nitrate, accelerate, inorganic nitrogen (such as NO3t-−N and NH4+−N) to be translated into organic nitrogen (such as protein and chlorophyll), and enhance the fresh weight and dry weights.
Biological Trace Element Research | 2007
Fan Yang; Chao Liu; Fengqing Gao; Mingyu Su; Xiao Wu; Lei Zheng; Fashui Hong; Ping Yang
The improvement of spinach growth is proved to relate to N2 fixation by nano-anatase TiO2 in this study. The results show that all spinach leaves kept green by nano-anatase TiO2 treatment and all old leaves of control turned yellow white under culture with N-deficient solution. And the fresh weight, dry weight, and contents of total nitrogen, % MathType!Translator!2!1!AMS LaTeX.tdl!TeX -- AMS-LaTeX! % MathType!MTEF!2!1!+- % feaaeaart1ev0aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbbjxAHX % garmWu51MyVXgatuuDJXwAK1uy0HwmaeHbfv3ySLgzG0uy0Hgip5wz % aebbnrfifHhDYfgasaacH8qrps0lbbf9q8WrFfeuY-Hhbbf9v8qqaq % Fr0xc9pk0xbba9q8WqFfea0-yr0RYxir-Jbba9q8aq0-yq-He9q8qq % Q8frFve9Fve9Ff0dmeaabaqaciGacaGaaeqabaWaaeWaeaaakeaaca % qGobGaaeisamaaDaaaleaacaaI0aaabaGaey4kaScaaaaa!3AF9!
Biological Trace Element Research | 2006
Fengqing Gao; Fashui Hong; Chao Liu; Lei Zheng; Mingyu Su; Xiao Wu; Fan Yang; Cheng Wu; Ping Yang
Biological Trace Element Research | 2006
Fashui Hong; Cheng Wu; Chao Liu; Kang Wu; Fengqing Gao; Fan Yang
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Biological Trace Element Research | 2006
Cheng Wu; Fashui Hong; Chao Liu; Yongling Wang; Kang Wu; Fengqing Gao; Fan Yang
Biometals | 2008
Fengqing Gao; Chao Liu; Chunxiang Qu; Lei Zheng; Fan Yang; Mingyu Su; Fashui Hong
, chlorophyll, and protein of spinach by nano-anatase TiO2 treatment presented obvious enhancement compared with control. Whereas the improvements of yield of spinach were not as good as nano-anatase TiO2 treatment under N-deficient condition, confirming that nano-anatase TiO2 on exposure to sunlight could chemisorb N2 directly or reduce N2 to NH3 in the spinach leaves, transforming into organic nitrogen and improving the growth of spinach. Bulk TiO2 effect, however, was not as significant as nano-anatase TiO2. A possible metabolism of the function of nano-anatase TiO2 reducing N2 to NH3 was discussed.
Biological Trace Element Research | 2007
Mingyu Su; Fashui Hong; Chao Liu; Xiao Wu; Xiaoqing Liu; Liang Chen; Fengqing Gao; Fan Yang; Zhongrui Li
Having a photocatalyzed characteristic, our previous research had proved that nano-anatase TiO2 is closely related to the photosynthesis of spinach. It could not only improve the light absorbance and the transformation from light energy to electron energy and to active chemical energy but also promote carbon dioxide (CO2) assimilation of spinach. However, the mechanism of carbon reaction promoted by nano-anatase TiO2 remains largely unclear. By electrophoresis and Western blot methods, the results of the experiments proved that Rubisco from the nano-anatase TiO2-treated spinach during the extraction procedure of Rubisco was found to consist of Rubisco and a heavier molecular-mass protein (about 1200 kDa) comprising both Rubisco and Rubisco activase. The Rubisco carboxylase activity was 2.67 times that of Rubisco from the control and it could hydrolyze ATP in the same manner as Rubisco activase. The total sulfhydryl groups and available sulfhydryl groups of the Rubisco were 32-SH and 21-SH per mole of enzyme more than those of the Rubisco purified from the control, respectively. The circular dichroism spectra showed that the secondary structure of Rubisco from the nano-anatase TiO2-treated spinach was very different from Rubisco of the control. It suggested that the mechanism of nano-anatase TiO2 activating Rubisco of spinach was that the complex of Rubsico and Rubisco activase was induced in spinach, which promoted Rubsico carboxylation and increased the rate of photosynthetic carbon reaction.
Biochemical and Biophysical Research Communications | 2006
Chao Liu; Fashui Hong; Kang Wu; Hong-bing Ma; Xueguang Zhang; Cheng-jiao Hong; Cheng Wu; Fengqing Gao; Fan Yang; Lei Zheng; Xuefeng Wang; Tao Liu; Yaning Xie; Jian-Hua Xu; Zhongrui Li
Cadmium is one of the most toxic heavy metals and is known to accumulate in freshwater food chains. The underlying mechanism for its genotoxicity has not been investigated for any freshwater fish. It has, however, been suggested that cadmium-induced carcinogenesis might involve either direct or indirect interaction of Cd2+ with DNA. The interaction between Cd2+ and DNA from the kidney of the silver crucian carp (Carassius auratus gibelio) in vitro and in vivo is investigated by spectrophotometric methods and agarose gel electrophoresis methods. Cd2+ could insert into DNA basepairs, bind to nucleic acid, and result in notable hypochromicities. The analysis of agarose gel electrophoresis, proves that Cd2+ at different concentrations does not cause DNA cleavage in vitro; however, kidneys display the classical laddering degradation of DNA in vivo, which is the result of the promotion of deoxyribonuclease activity or inhibition of superoxide dismutase and catalyse activity and the accumulation of reactive oxygen species caused by Cd2+ ions in vivo.
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2008
Xiao Wu; Fashui Hong; Chao Liu; Mingyu Su; Lei Zheng; Fengqing Gao; Fan Yang
The mechanism of Cd2+ on the DNA cleavage and Ce3+ on the DNA repair in the kidney of silver crucian carp (Carassius auratus gibelio) is investigated by agarose gel electrophoresis methods and assaying biochemical indexes. It proves that Cd2+ induces the classical laddering degradation of DNA in vivo, but DNA cleavage is repaired after injecting with a low Ce3+ concentration under various Cd2+ concentrations. The DNA cleavage caused by Cd2+ is the result of the activation of deoxyribonuclease (DNase) and accumulation of reactive oxygen species (ROS), and Cd2+ destroys the antioxidant system, which diminishes the activities of superoxide dismutase, catalase, and peroxidase, and the increase of the lipid peroxidation (LPO) level. However, Ce3+ could inhibit activation of Cd2+ on DNase activity, relieve inhibition of Cd2+ on activities of the antioxidant enzyme, and diminish ROS accumulation. The results show that Ce3+ could relieve the toxicity of Cd2+ to silver crucian carp.
Fish Physiology and Biochemistry | 2008
Cheng Wu; Ling Wang; Chao Liu; Fengqing Gao; Mingyu Su; Xiao Wu; Fashui Hong