Ferenc Boldog
Karolinska Institutet
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Featured researches published by Ferenc Boldog.
Genomics | 1991
Eugene R. Zabarovsky; Ferenc Boldog; Rando L. Allikmets; Zoltan Marcsek; Lev L. Kisselev; Eric J. Stanbridge; George Klein; Janos Sümegi; Gösta Winberg
A novel procedure for construction of jumping libraries is described. The essential features of this procedure are as follows: (1) two diphasmid vectors (lambda SK17 and lambda SK22) are simultaneously used in the library construction to improve representativity, (2) a partial filling-in reaction is used to eliminate cloning of artifactual jumping clones and to obviate the need for a selectable marker. The procedure has been used to construct a representative human NotI jumping library (220,000 independent recombinant clones) from the lymphoblastoid cell line CBMI-Ral-STO, which features a low level of methylation of its resident EBV genomes. A human chromosome 3-specific NotI jumping library (500,000 independent recombinant clones) from the human chromosome 3 x mouse hybrid cell line MCH 903.1 has also been constructed. Of these recombinant clones 50-80% represent jumps to the neighboring cleavable NotI site. With our previously published method for construction of linking libraries this procedure makes a new genome mapping strategy feasible. This strategy includes the determination of tagging sequences adjacent to NotI sites in random linking and jumping clones. Special features of the lambda SK17 and lambda SK22 vectors facilitate such sequencing. The STS (sequence tagged site) information obtained can be assembled by computer into a map representing the linear order of the NotI sites for a chromosome or for the entire genome. The computerized mapping data can be used to retrieve clones near a region of interest. The corresponding clones can be obtained from the panel of original clones, or necessary probes can be made from genomic DNA by PCR.
Cytogenetic and Genome Research | 1991
Josiane Szpirer; Mozaffarul Islam; Ferenc Boldog; George Klein; S Ingvarsson
It has previously been shown that human chromosome 3 has banding homology to rat chromosome 8. We have previously isolated a cDNA from the D3F15S2 region and designated the gene as RIK. In the present study, we localized the homolog of this gene to rat chromosome 8.
Cancer Genetics and Cytogenetics | 1989
Ferenc Boldog; George Klein; Janos Sümegi
Physical maps have been constructed around loci DNF15S2, D3S2, and c-raf1 on the short arm of human chromosome 3 using pulsed field gradient gel electrophoresis. The normal restriction pattern has not been altered by a t(3;8)(p14.2;q24.1) characteristic for a hereditary form of renal cell carcinoma, indicating that the breakpoint itself is not included in any of the mapped areas. We have found a CpG island within the DNF15S2 locus, suggesting the presence of a functional gene in the region.
Oncogene | 1991
Ferenc Boldog; Bengt Persson; Eugene R. Zabarovsky; Rando L. Allikmets; Janos Sümegi; George Klein; Hans Jörnvall
Nucleic Acids Research | 1990
Eugene R. Zabarovsky; Ferenc Boldog; Teryl Thompson; David Scanlon; Gösta Winberg; Zoltan Marcsek; Eric J. Stanbridge; George Klein; Janos Sümegi
FEBS Letters | 1997
Rinat Gizatullin; A. I. Protopopov; Rando Allikmets; S Korolev; Jingfeng Li; Ferenc Boldog; K Tory; Veronika Zabarovska; Z. Marcsek; J Sumegi; George Klein; Eugene R. Zabarovsky; Lev L. Kisselev
Oncogene | 1990
S Ingvarsson; Ulf S.R. Bergerheim; Ferenc Boldog; Zoltan Marcsek; Kazutu Kunimi; Bryan Y.-T. Lin; Javier Saez Castresana; Wen-Hwa Lee; Eva Y.-H. P. Lee; George Klein; Janos Sümegi
Cancer Genetics and Cytogenetics | 1988
Ferenc Boldog; Janos Sümegi; George Klein
Genomics | 1993
Eugene R. Zabarovsky; Ekaterina S. Pokrovskaya; Veronika Zabarovska; Ji-Yi Wang; Peter Berglund; Ferenc Boldog; Eric J. Stanbridge; Janos Sümegi; George Klein; Gösta Winberg
Genes, Chromosomes and Cancer | 1991
Ferenc Boldog; Kristina Arheden; Stephan Imreh; Bodil Strömbeck; Laszlo Szekely; Zoltan Marcsek; Janos Sümegi; Felix Mitelman; George Klein