Fernanda Timm Seabra Souza
Universidade Federal do Rio Grande do Sul
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Fernanda Timm Seabra Souza.
International Journal of Developmental Neuroscience | 2018
Tatiane Grazieli Hammerschmidt; Graziela de Oliveira Schmitt Ribas; Maria Luiza Saraiva-Pereira; Márcia Polese Bonatto; Rejane Gus Kessler; Fernanda Timm Seabra Souza; Franciele Barbosa Trapp; Kristiane Michelin-Tirelli; Maira Graeff Burin; Roberto Giugliani; Carmen Regla Vargas
Niemann‐Pick type C (NP‐C), one of 50 inherited lysosomal storage disorders, is caused by NPC protein impairment that leads to unesterified cholesterol accumulation in late endosomal/lysosomal compartments. The clinical manifestations of NP‐C include hepatosplenomegaly, neurological and psychiatric symptoms. Current diagnosis for NP‐C is based on observation of the accumulated cholesterol in fibroblasts of affected individuals, using an invasive and time expensive test, called Filipin staining. Lately, two metabolites that are markedly increased in NP‐C patients are arising as biomarkers for this disease screening: 7‐ketocholesterol and cholestane‐3β,5α,6β‐triol, both oxidized cholesterol products.
Genetics and Molecular Biology | 2004
Fernanda Timm Seabra Souza; Alexandre Silva de Mello; Kristiane Michelin; Janice Carneiro Coelho
The effect of four antibiotics (amikacin, clindamycin, cephalothin and vancomycin) was investigated considering that bacterial infection in fibroblasts cultures is a very frequent event. The investigation included the effect of the antibiotics on fibroblast growth and on the activity of the enzyme glucocerebrosidase. The antibiotics were added to the fibroblast cultures and cell growth was evaluated by counting the number of cells and their viability. After cell harvesting, the enzyme activity and content of protein were measured. The results allowed us to conclude that none of the antibiotics affected the cellular number nor the cellular viability. The content of protein decreased when cephalothin and clindamycin were added to the cultures, and glucocerebrosidase was affected in the presence of amikacin. Vancomycin did not interfere with any of the parameters analyzed, so it was chosen to be used in cell cultures to prevent the contamination by gram positive bacteria.
Revista Argentina De Microbiologia | 2010
Fernanda Timm Seabra Souza; Luana Souza Sostruznik; Roberta Casagrande Scolari; Karen Joana Maciel de Castro; Carla Vieira Andrade; Roberto Giugliani; Janice Carneiro Coelho
This study was designed to evaluate the effect of mycoplasma contamination on acid hydrolase activity and the action of the mycoplasma removal agent (MRA), in cultures of human fibroblasts from individuals with lysosomal diseases. For this purpose, we measured the activity of the b-galactosidase, arylsulphatase B (ASB), hexosaminidase A and a-glucosidase enzymes. The activity of the above mentioned enzymes in fibroblasts contaminated by mycoplasma was measured before and after the addition of the MRA. The results were then compared to the enzymatic activity in contamination-free cultures. Only the ASB enzyme showed significant alteration in activity both in the presence of mycoplasma and MRA. The remaining enzymes did not suffer significant interference by the presence of the two agents. Of the four enzymes tested, three did not suffer significant alterations by the presence of the mycoplasma nor from the MRA. However, the activity measured in the ASB enzyme increased significantly in the presence of mycoplasma and MRA and could lead to a doubtful diagnosis. Therefore, we suggest that contamination should be prevented by using aseptic techniques as well as the MRA in those fibroblast cultures that cannot be discarded.
Clinical Biochemistry | 2007
Fernanda Timm Seabra Souza; Luana Souza Sostruznik; Roberta Casagrande Scolari; Karen Joana Maciel de Castro; Roberto Giugliani; Janice Carneiro Coelho
Arquivos De Neuro-psiquiatria | 2014
Paulo José Lorenzoni; Elaine H. Cardoso; Ana Crippa; Charles Marques Lourenço; Fernanda Timm Seabra Souza; Roberto Giugliani; Maria Luiza Saraiva-Pereira; Salmo Raskin; Isac Bruck; Cláudia Suemi Kamoi Kay; Rosana Herminia Scola; Lineu Cesar Werneck; Hélio A.G. Teive
Archive | 2016
Ana Carolina Silva Rodrigues Farias; Márcia Polese Bonatto; Hugo Bock; Maria Cristina Cotta Matte; Mirela Severo Gil; Fernanda Timm Seabra Souza; Rejane Gus Kessler; Roberto Giugliani; Maria Luiza Saraiva Pereira
Archive | 2015
Ana Carolina Silva Rodrigues Farias; Márcia Polese Bonatto; Hugo Bock; Maria Cristina Cotta Matte; Mirela Severo Gil; Fernanda Timm Seabra Souza; Rejane Gus Kessler; Roberto Giugliani; Maria Luiza Saraiva Pereira
Molecular Genetics and Metabolism | 2015
Roberto Giugliani; Graziela S. Ribas; Gabriel Civallero; Heryk Motta de Souzal; Maira Graeff Burin; Kristiane Michelin-Tirelli; Maria Luiza Saraiva-Pereira; Márcia Polese-Bonatto; Mirela Severo Gil; Rejane Gus Kessler; Fernanda Timm Seabra Souza; Talita Freitas; Carolina Fischinger Moura de Souza; Filippo Pinto e Vairo; Célio Luiz Rafaelli; Franciele Barbosa Trapp; Carmen Regla Vargas
Archive | 2014
Maria Luiza Saraiva Pereira; Roberto Giugliani; Rejane Gus Kessler; Fernanda Timm Seabra Souza; Mirela Severo Gil; Rafaella Mergener; Maria Cristina Cotta Matte; Hugo Bock; Márcia Polese Bonatto
Archive | 2014
Mirela Severo Gil; Márcia Polese Bonatto; Hugo Bock; Maria Cristina Cotta Matte; Rafaella Mergener; Fernanda Timm Seabra Souza; Rejane Gus Kessler; Roberto Giugliani; Maria Luiza Saraiva Pereira