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Dive into the research topics where Filippe E. F. Soares is active.

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Featured researches published by Filippe E. F. Soares.


Química Nova | 2014

Otimização do pré-tratamento hidrotérmico da palha de cana-de-açúcar visando à produção de etanol celulósico

Fernando Santos; José Humberto de Queiroz; Jorge Luiz Colodette; Mauro Manfredi; Maria Eliana; L. R. Queiroz; Filippe E. F. Soares

The sugarcane industry has huge potential for biorefinery concept application, given its development in recent years. In this context, cane sugar straw has become an attractive raw material for biofuel production. This study aims to investigate the chemical composition of cane sugar straw from different regions of Brazil, and to optimize a hydrothermal pretreatment stage for cellulosic ethanol production. Results of chemical characterization of the cane sugar straw for the regions assessed indicated little influence of place on straw chemical composition. Hydrothermal pretreatment showed high efficiency in hemicellulose removal. Hydrothermal pretreatments operating with temperatures of 190 and 210 oC presented satisfactory results, reaching values close to 100% hydrolysis.


Veterinary Parasitology | 2010

Ovicidal action of a crude enzymatic extract of the fungus Pochonia chlamydosporia against cyathostomin eggs.

Fabio Ribeiro Braga; Jackson Victor de Araújo; Rogério Oliva Carvalho; André R. Silva; Juliana Milani Araujo; Filippe E. F. Soares; Hugo Leonardo André Geniêr; Sebastião Rodrigo Ferreira; José Humberto de Queiroz

The aims of this study were to test the action of the fungal extract of Pochonia chlamydosporia (VC4) on the hatching of cyathostomin eggs plated in Petri dishes containing 2% water-agar (2% WA) and its enzymatic activity in fecal cultures, in two experimental assays (A and B). The fungus P. chlamydosporia (VC4) was cultured in Erlenmeyer flasks (250ml) containing 50ml of liquid minimal medium supplemented with 0.2% gelatin for production of the crude enzymatic extract. Approximately 1kg of fresh feces was collected directly from the rectum of crossbred horses naturally infected with cyathostomins. The fecal material was used to obtain eggs and prepare fecal cultures. For assay A, one thousand eggs were plated on 4.5cm diameter Petri dishes together with 5ml of VC4 fungal filtrate and incubated at 26 degrees C in the dark for 24h. The control group consisted of 1000 eggs in Petri dishes containing 10ml of distilled water, which were incubated under the same conditions. After 24h, the total number of cyathostomin larvae present in each plate of the treated and control groups was counted. For assay B, about 20g of feces were added with 10ml of fungal extract of P. chlamydosporia (VC4) and incubated at 26 degrees C for 8 days. Third stage larvae (L(3)) were recovered at the end of this period. Significant difference (p<0.01) was found for the number of larvae between the treated group and the control at end of assay A. A 72.8% reduction in the hatching of cyathostomin eggs was found in the plates of the treated group compared with the control group. At the end of 8 days, the fungal extract of P. chlamydosporia (VC4), in assay B, was effective in reducing the number of L(3) cyathostomins in the treated group by 67.0% compared with the control group. Significant difference (p<0.01) was found between the means of L(3) recovered from the treated group and the control group. The results of this work showed that crude enzymatic extract of P. chlamydosporia (VC4) was effective in reducing hatching of cyathostomin eggs and therefore could be used as a biological control agent of this nematode.


Biocontrol Science and Technology | 2012

An extracellular serine protease of an isolate of Duddingtonia flagrans nematophagous fungus

Fabio Ribeiro Braga; Jackson Victor de Araújo; Filippe E. F. Soares; Hugo Leonardo André Geniêr; José Humberto de Queiroz

Abstract This study aimed to present a protease produced by Duddingtonia flagrans fungus (AC001), and to evaluate its activity in the biological control of cyathostomin infective larvae (L3). The crude extract from D. flagrans grown in liquid medium was applied first to a DEAE-Sepharose™ and later to a CM-Sepharose™ ion exchange column. Protease activity was determined under different pHs and temperatures. Subsequently, the effects of metal ions and phenylmethylsulfonyl fluoride (PMSF) inhibitor on activity were evaluated. Next, the protease activity in the biological control of nematodes was tested. A new 38 kDa serine protease (Df1) was purified. Optimum activity was obtained at pH 8.0 and 60°C; CuSO4, ZnSO4 and PMSF strongly inhibited the activity. Df1 (AC001) showed an L3 reduction rate of 58%. In conclusion, a serine protease produced by D. flagrans (AC001) has been isolated, which is effective in the in vitro destruction of cyathostomin L3.


Biocontrol Science and Technology | 2012

Enzymatic analysis and in vitro ovicidal effect of Pochonia chlamydosporia and Paecilomyces lilacinus on Oxyuris equi eggs of horses

Fabio Ribeiro Braga; Jackson Victor de Araújo; Filippe E. F. Soares; Alexandre de Oliveira Tavela; Juliana Milani Araujo; Rogério Oliva Carvalho; Fernanda Mara Fernandes; José Humberto de Queiroz

Abstract The aim of this work was to analyse the enzymatic activity and in vitro ovicidal effect of Pochonia chlamydosporia (VC1 and VC4) and Paecilomyces lilacinus (PL1) on Oxyuris equi eggs of horses. The growth of isolates and their enzymatic production were evaluated on agar media supplemented with gelatin (GA), casein (CA), olive oil (OOA) or starch (SSA). The ovicidal effect was evaluated according to morphological alterations. Following, the P. chlamydosporia crude extract production and proteolytic content was evaluated (VC1 and VC4) in liquid medium at the interval of 15, 30, 45 and 60 min during incubation of P. chlamydosporia and P. lilacinus which grew and showed enzymatic activity on agar media (GA, CA, OOA and SSA). At the 15th day, VC1, VC4 and PL1 showed results on GA, CA, OOA and SSA media, for Type 3 effect of (78, 54, 52 and 68%), (72, 50, 58 and 78%) and (62, 57, 65 and 63%). Pochonia chlamydosporia was able to grow in liquid medium (gelatin) and at Day 5 showed proteolytic activity. The results of the present work suggest that P. chlamydosporia and P. lilacinus can be used in the biological control of O. equi eggs.


Research in Veterinary Science | 2012

Survival of Pochonia chlamydosporia in the gastrointestinal tract of experimentally treated dogs.

Juliana Milani Araujo; Jackson Victor de Araújo; Fabio Ribeiro Braga; Dayane M. Araújo; Sebastião Rodrigo Ferreira; Filippe E. F. Soares; Laércio dos Anjos Benjamin

The predatory capacity of the nematophagous fungus Pochonia chlamydosporia (isolate VC4) after passage through the gastrointestinal tract of dogs was assessed in vivo against Toxocara canis eggs. Twelve dogs previously wormed were divided into two groups of six animals and caged. The treatments consisted of a fungus-treated group (VC4) and a control group without fungus. Each dog of the fungus-treated group received a single 4 g dose of mycelial mass of P. chlamydosporia (VC4). Fecal samples from animals of both groups (treated and control) were collected at five different times (6, 12, 24, 36, and 48 h) after fungal administration, and placed in Petri dishes. Each Petri dish of both groups for each studied time interval received approximately 1000 T. canis eggs. Thirty days after the fecal samples were collected, approximately one hundred eggs were removed from each Petri dish of each studied time interval and evaluated by light microscopy (LM) and scanning electron microscopy (SEM). Microscopy examination of plates inoculated with the fungus showed that the isolate VC4 was able to destroy the T. canis eggs with destruction percentages of 28.6% (6 h), 29.1% (12 h), 32.0% (24 h), 31.7% (36 h), and 37.2% (48 h). These results suggest that P. chlamydosporia can be used as a tool for the biological control of T. canis eggs in feces of contaminated dogs.


Anais Da Academia Brasileira De Ciencias | 2018

Statistical tools application on dextranase production from Pochonia chlamydosporia (VC4) and its application on dextran removal from sugarcane juice

Bruna Leite Sufiate; Filippe E. F. Soares; Angélica de Souza Gouveia; Samara Silveira Moreira; Evandro Ferreira Cardoso; Gabriella Peterlini Tavares; Fabio Ribeiro Braga; Jackson Victor de Araújo; José Humberto de Queiroz

The aim of this study was to optimize the dextranase production by fungus Pochonia chlamydosporia (VC4) and evaluate its activity in dextran reduction in sugarcane juice. The effects, over the P. chlamydosporia dextranase production, of different components from the culture medium were analyzed by Plackett-Burman design and central composite design. The response surface was utilized to determine the levels that, among the variables that influence dextranase production, provide higher production of these enzymes. The enzymatic effect on the removal of dextran present in sugarcane juice was also evaluated. It was observed that only NaNO3 and pH showed significant effect (p<0.05) over dextranase production and was determined that the levels which provided higher enzyme production were, respectively, 5 g/L and 5.5. The dextranases produced by fungus P. chlamydosporia reduced by 75% the dextran content of the sugarcane juice once treated for 12 hours, when compared to the control treatment.


Parasitology Research | 2012

In vitro activity of a serine protease from Monacrosporium thaumasium fungus against first-stage larvae of Angiostrongylus vasorum

Filippe E. F. Soares; Fabio Ribeiro Braga; Jackson Victor de Araújo; Walter dos Santos Lima; Lanuze R. Mozer; José Humberto de Queiroz


Parasitology Research | 2013

Nematicidal activity of three novel extracellular proteases of the nematophagous fungus Monacrosporium sinense

Filippe E. F. Soares; Fabio Ribeiro Braga; Jackson Victor de Araújo; Hugo Leonardo André Geniêr; Angélica de Souza Gouveia; José Humberto de Queiroz


BMC Research Notes | 2013

Mycelial mass production of fungi Duddingtonia flagrans and Monacrosporium thaumasium under different culture conditions.

Manoel Eduardo da Silva; Jackson Victor de Araújo; Fabio Ribeiro Braga; Luana Alcântara Borges; Filippe E. F. Soares; Walter dos Santos Lima; Marcos Pezzi Guimarães


Tropical Animal Health and Production | 2014

Predatory capability of the nematophagous fungus Arthrobotrys robusta preserved in silica gel on infecting larvae of Haemonchus contortus

Fabio Ribeiro Braga; Rogério Oliva Carvalho; André R. Silva; Jackson Victor de Araújo; Luiza Neme Frassy; Andrea Lafisca; Filippe E. F. Soares

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Fabio Ribeiro Braga

Universidade Federal de Viçosa

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Juliana Milani Araujo

Universidade Federal de Viçosa

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Rogério Oliva Carvalho

Universidade Federal de Viçosa

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André R. Silva

Universidade Federal de Viçosa

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Luiza Neme Frassy

Universidade Federal de Viçosa

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