Frances Kim

MP35-10 DETECTION OF PROSTATE CANCER-ASSOCIATED TRANSCRIPTS IN URINARY EXTRACELLULAR VESICLES

PC 01 28 PC 11 30 PC 01 35 PC 01 16 PC 10 53 PC 10 31 PC 10 08 PC 10 82 PC 11 93 PC 01 34 PC 01 27 PC 01 11 PC 11 67 PC 11 26 PC 01 30 PC 00 46 PC 01 00 PC 01 43 PCA3|PolyA_1.LM2 CXCL14|Reference_end.LM1 SPINK1|Reference_end.LM1 STK36|Reference_end.LM2 ANO7|Reference_end.LM2 TMEM144|Reference_end.LM2 TNFRSF19|Reference_end.LM2 AMACR|PolyA_1.LM2 GPI|PolyA_1.LM2 CAMKK2|Reference_end.LM2 MYBPC1|Reference_end.LM1 EDRF1|Reference_end.LM1 EPCAM|Reference_end.LM2 ANK3|PolyA_1.LM2 SPAG5|Reference_end.LM2 BTG2|Reference_end.LM2 TMEM45B|PolyA_1.LM1 IPO4|Reference_end.LM1 RABGAP1|Reference_end.LM2 ISX|Reference_end.LM2 PSMB2|PolyA_1.LM2 PSMB2|PolyA_1.LM1 SLC25A5|PolyA_1.HE RPL30|Reference_end.HE SOX4|Reference_end.LM1 CLTC|PolyA_2.LM2 DANCR|Reference_end.LM1 SMARCE1|PolyA_1.LM1 −1 −0.5 0 0.5 1 Value 0 60 Color Key and Histogram

MP100-16 LAPAROSCOPIC RENAL CRYOABLATION USING REAL-TIME INTRAOPERATIVE THERMAL MONITORING: 15 YEAR EXPERIENCE

effective, and non-invasive tissue ablation modality. Previous studies have shown that HIFU delivered laparoscopically can be used to ablate kidney tumors, potentially enabling a lower morbidity treatment with faster recovery time as compared to partial nephrectomy procedures. Challenges remain, however, including ensuring full tissue necrosis and consistent energy coupling to the target volume. The objective of this study was to evaluate whether a newly developed laparoscopic HIFU probe is able to address these challenges. METHODS: A laparoscopic porcine kidney model was used to investigate the safety and efficacy of the new 15mm HIFU probe. Under ultrasound guidance, kidneys of 12 pigs were targeted and ablated with HIFU, creating on average 2 ablation zones per kidney of varying sizes and locations in order to quantify the probe0s ability to deliver HIFU to any location on the kidney. Efficacy was evaluated via the analysis of ablation volume histology slides, real-time ultrasound images collected during HIFU delivery, and MRI and ultrasound contrast images. Safety was evaluated by surviving a subgroup of the animals (2w). Grosspathological data, sonication parameters, and workflow feedback was also collected during the study. RESULTS: Repeatable lesions could be created at a rate of 0.48cm/min and average energy densities of 584cal/cm. Histological evaluation indicated contiguous ablated volumes using these delivery parameters, extending from the transducer0s focal zone to the kidney surface, with a maximum treatment depth of 27mm. Ablated target volumes ranged from 5.1cm to 24.5cm. CONCLUSIONS: The results confirm the ability of the new probe to deliver HIFU in a consistent and reliable manner. Initial dose requirements for ablating tissue at various depths were also determined. Workflow feedback has resulted in additional system user interface improvements, with all of these results paving the way for a future clinical study.