Francesca Cimino

Eumelanin Coated PLA Electrospun Micro Fibers as Bioinspired Cradle for SH-SY5Y Neuroblastoma Cells Growth and Maturation

Within the framework of neurodegenerative disorder therapies, the fabrication of 3D eumelanin architectures represents a novel strategy to realize tissue-engineering scaffolds for neuronal cell growth and control by providing both mechanical support and biological signals. Here, an appropriate procedure combining electrospinning, spin coating and solid-state polymerization process is established to realize the scaffolds. For biological analysis, a human derived cell line SH-SY5Y from neuroblastoma is used. Cell maturation on eumelanin microfibers, random and aligned, is evaluated by using confocal analysis and specific markers of differentiating neurons (βIII tubulin and GAP-43 expression). Cell morphology is tested by SEM analysis and immunofluorescence techniques. As results, eumelanin coated microfibers prove capable to support biological response in terms of cell survival, adhesion and spreading and to promote cell differentiation toward a more mature neuronal phenotype as confirmed by GAP-43 expression over the culture.

Poly(Butylene Terephthalate) Based Composites Containing Alumina Whiskers: Influence of Filler Functionalization on Dielectric Properties

Poly(butylene terephthalate) (PBT) is one of the most widely used semicrystalline thermoplastics polyester because of its superior thermal and mechanical properties, high dimensional stability and excellent processability. In this research PBT-based nanocomposites, including various amounts (up to 10 wt%) of commercial alumina whiskers, have been prepared by using a Brabender internal chamber mixer and analysed in terms of morphological features and dielectric properties. Specific attention has been focused on the effect of the filler functionalization considering 3-glycidoxy propylmethoxysilane (GPS) or 3-methacryloxypropyltrimethoxysilane (MPS) as coupling agents. Tests, performed on compounds filled with neat and functionalized alumina whiskers, show a clear dependence of relative dielectric permittivity , invariance of dissipation factor (), and a sensible increase of volume electrical resistivity with the filler’s content and are encouraging for a future introduction of such composites in many electrical applications.

Low velocity impact behaviour of polypropylene and epoxy composite laminates reinforced with a basalt woven fabric

Woven basalt fiber composite laminates based on epoxy and polypropylene (PP) resins were dynamically loaded by a falling weight machine, by using a cylindrical impactor with a hemispherical nose. Laminates with the same thickness were investigated to compare their dynamic response. Impact tests were carried out at penetration to obtain the complete load-displacement curve and to measure the penetration energy, and at different energy levels to study the influence of the matrix on the damage start and propagation. A general better behavior of epoxy basalt fibre reinforced plates (BFRP) was noted while, surprisingly, the polypropylene based laminates didn’t show any penetration even at high impact energy highlighting the importance of this study.Woven basalt fiber composite laminates based on epoxy and polypropylene (PP) resins were dynamically loaded by a falling weight machine, by using a cylindrical impactor with a hemispherical nose. Laminates with the same thickness were investigated to compare their dynamic response. Impact tests were carried out at penetration to obtain the complete load-displacement curve and to measure the penetration energy, and at different energy levels to study the influence of the matrix on the damage start and propagation. A general better behavior of epoxy basalt fibre reinforced plates (BFRP) was noted while, surprisingly, the polypropylene based laminates didn’t show any penetration even at high impact energy highlighting the importance of this study.

Application of optical interferometric techniques for non-destructive evaluation of novel "green" composite materials

Nowadays the use of advanced composite materials in aeronautics, both civil and military, in automotive and in sport applications, citing some, is well established. The characteristics of composite materials in terms of weight, fatigue resistance and corrosion resistance make them competitive with respect to conventional ones. On the other side, the fabrication process of the most employed composites reinforced by carbon fibers or glass fibers, needs of complex steps that not always are environmental complaisant. Moreover, such fibers are not themselves “green”. For these reasons, in the last decades, the use of natural reinforcing fibers has gained an increasing attention allowing the development of new materials with the same advantages of composite systems but respecting the environment. Furthermore, such materials for their structural complexity are not always compatible with the use of standard non-destructive evaluation as the ultrasounds methods. In this work the efficiency of the employment of optical interferometric techniques as nondestructive evaluation methods in full field modality is proved on novel “green” composite materials. In particular, Electronic Speckle Pattern Interferometry has been tested on different kinds of specimens after flexural tests.

Electrospun Drug-Loaded Textiles for Biomedical and Healthcare Applications

Electrospinning is a versatile technology for the production of polymer micro/nanoscale fibers. It provides the direct addition of bioactive payloads into micro/nanofibers thus improving the encapsulation efficiency and reducing the burst release via proper selection of drug-polymer-solvent system or electrospinning setup. In addition, since electrospun fibers have one dimension at the microscopic scale but another dimension at the macroscopic one, it is possible to combine the advantages possessed by functional materials on the nanometer scale. A large surface to volume ratio, with the properties of conventional solid membranes, such as ease of manipulation and applicability in any size and shape, making them suitable for biomedical or healthcare applications both topically (i.e. skin) and locally (i.e. tumor). This communication deals with electrospun fibrous systems containing active compounds extracts from plants such as Artemisinin and Propolis; these systems have been properly realized to preserve the pharmacological activity of drugs and evaluated as potential delivery systems for vector-born deseases and cancer, reducing the damage to non-target organism and environment.