Francielli Pantella Kunz de Jesus
Universidade Federal de Santa Maria
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Veterinary Microbiology | 2011
Francielli Pantella Kunz de Jesus; Claudia Lautert; Régis Adriel Zanette; D.L. Mahl; Maria Isabel de Azevedo; M.L.S. Machado; Valéria Dutra; Sônia de Avila Botton; Sydney Hartz Alves; Jânio Morais Santúrio
OBJECTIVES The first aim of this study was to evaluate the in vitro efficacies of fluconazole, ketoconazole, itraconazole and voriconazole on M. pachydermatis growth inhibition. This study also evaluated M. pachydermatis azole cross-resistance, comparing wild clinical isolates and the same isolates with in vitro-induced fluconazole resistance. METHODS Two techniques were used: (1) a broth microdilution method based on protocol M27-A3 from the Clinical and Laboratory Standards Institute to determine the minimum inhibitory concentration (MIC) and (2) the Fekete-Forgács method to induce fluconazole resistance in vitro. The isolates were divided into two groups: group 1 included fluconazole-susceptible clinical isolates (n=30) and group 2 contained the same isolates with in vitro-induced fluconazole resistance (n=30). RESULTS The two groups exhibited differences in susceptibility (p<0.001). Group 1 isolates were susceptible to azoles: ketoconazole (MIC 0.01-1.0 μg/mL), itraconazole (MIC 0.01-1.0 μg/mL), voriconazole (MIC 0.01-4.0 μg/mL), and fluconazole (MIC 0.01-4.0 μg/mL). Group 2 isolates demonstrated a wider range of MICs to azoles: ITZ (MIC 0.06-64.0 μg/mL), KTZ (MIC 0.25-32.0 μg/mL), VRZ (MIC 2.0-128.0 μg/mL), and FLZ (MIC 64.0-128.0 μg/mL). CONCLUSIONS It was shown that FLZ-resistant M. pachydermatis isolates exhibit cross-resistance to other azoles, reinforcing the importance of susceptibility tests as a guide for the therapeutic prescription of antifungals in medical and veterinary mycology.
Veterinary Microbiology | 2012
Maria Isabel de Azevedo; Sônia de Avila Botton; Daniela Isabel Brayer Pereira; Lizandra J. Robe; Francielli Pantella Kunz de Jesus; Camila D. Mahl; Mateus Matiuzzi da Costa; Sydney Hartz Alves; Jânio Morais Santúrio
Pythium insidiosum is an aquatic oomycete that is the causative agent of pythiosis. Advances in molecular methods have enabled increased accuracy in the diagnosis of pythiosis, and in studies of the phylogenetic relationships of this oomycete. To evaluate the phylogenetic relationships among isolates of P. insidiosum from different regions of Brazil, and also regarding to other American and Thai isolates, in this study a total of thirty isolates of P. insidiosum from different regions of Brazil was used and had their ITS1, 5.8S rRNA and ITS2 rDNA (ITS) region and the partial sequence of cytochrome oxidase II (COX II) gene sequenced and analyzed. The outgroup consisted of six isolates of other Pythium species and one of Lagenidium giganteum. Phylogenetic analyses of ITS and COX II genes were conducted, both individually and in combination, using four different methods: Maximum parsimony (MP); Neighbor-joining (NJ); Maximum likelihood (ML); and Bayesian analysis (BA). Our data supported P. insidiosum as monophyletic in relation to the other Pythium species, and COX II showed that P. insidiosum appears to be subdivided into three major polytomous groups, whose arrangement provides the Thai isolates as paraphyletic in relation to the Brazilian ones. The molecular analyses performed in this study suggest an evolutionary proximity among all American isolates, including the Brazilian and the Central and North America isolates, which were grouped together in a single entirely polytomous clade. The COX II network results presented signals of a recent expansion for the American isolates, probably originated from an Asian invasion source. Here, COX II showed higher levels bias, although it was the source of higher levels of phylogenetic information when compared to ITS. Nevertheless, the two markers chosen for this study proved to be entirely congruent, at least with respect to phylogenetic relationships between different isolates of P. insidiosum.
Brazilian Journal of Microbiology | 2013
Caroline Borges Weiler; Francielli Pantella Kunz de Jesus; Graziela Habib Nardi; Érico Silva Loreto; Janio Morais Santurio; Selene Dall’ Acqua Coutinho; Sydney Hartz Alves
Malassezia pachydermatis is associated with dermatomycoses and otomycosis in dogs and cats. This study compared the susceptibility of M. pachydermatis isolates from sick (G1) and healthy (G2) animals to azole and polyene antifungals using the M27-A3 protocol. Isolates from G1 animals were less sensitive to amphotericin B, nystatin, fluconazole, clotrimazole and miconazole.
Diagnostic Microbiology and Infectious Disease | 2018
Laura Bedin Denardi; Francielli Pantella Kunz de Jesus; Jéssica Tairine Keller; Carla Weiblen; Maria Isabel de Azevedo; Vanessa Oliveira; Janio Morais Santurio; Sydney Hartz Alves
Posaconazole (PSC) in combination with anidulafungin (AFG) was evaluated in a murine model of pulmonary aspergillosis. Immunosuppressed animals were infected via the nasal cavity with 2 different A. fumigatus strains. The animals received PSC (oral, 20mg/kg per day) and/or AFG (i.p., 10mg/kg per day) for 7days. On Day 8, the mice were euthanized and fungal burdens were determined from the lungs. Survival curves were constructed for mortality analysis. Compared to untreated groups, groups singly treated with PSC or AFG showed a reduced fungal burden in the lungs (P=0.0001-0.006) and prevention of mortality (66.66-83.33% of survival). Combination treatment with PSC and AFG significantly reduced the fungal burden (or sterilized the lungs) compared to the findings in the untreated and monotherapy groups and improved the survival rate to 100%. The PSC and AFG combination therapy was highly effective and should be evaluated in larger-scale experiments.
Revista Da Sociedade Brasileira De Medicina Tropical | 2017
Débora Alves Nunes Mario; Larissa Finger Schaffer; Luis Ricardo Peroza; Francielli Pantella Kunz de Jesus; Laura Bedin Denardi; Roselei Fachinetto; Sydney Hartz Alves
INTRODUCTION: We compared indicators of oxidative stress in the tissue of mice infected with strains from Sporothrix schenckii complex. METHODS: Mice were inoculated with Sporothrix brasiliensis, Sporothrix schenckii sensu stricto, Sporothrix globosa, Sporothrix mexicana or Sporothrix albicans. The activity of catalase and glutathione were accessed in the liver and spleen. RESULTS: Animals infected with S. brasiliensis exhibited splenomegaly and significant decrease in catalase activity, and protein and non-protein thiol content compared to animals infected with the other species. CONCLUSIONS: Sporothrix brasiliensis exhibits higher pathogenicity compared to other species of the Sporothrix schenckii complex by increasing oxidative stress in animal tissue.
Brazilian Journal of Infectious Diseases | 2017
Laura Bedin Denardi; Bianca Hoch Dalla-Lana; Francielli Pantella Kunz de Jesus; Cecília Bittencourt Severo; Janio Morais Santurio; Régis Adriel Zanette; Sydney Hartz Alves
The in vitro susceptibility of 105 clinical and environmental strains of Aspergillus fumigatus and Aspergillus flavus to antifungal drugs, such as amphotericin B, azoles, and echinocandins was evaluated by the broth microdilution method proposed by the European Committee on Antimicrobial Susceptibility Testing (EUCAST). Following the EUCAST-proposed breakpoints, 20% and 25% of the clinical and environmental isolates of A. fumigatus, respectively, were found to be resistant to itraconazole (Minimal Inhibitory Concentration, MIC>2.0mg/L). Voriconazole showed good activity against A. fumigatus and A. flavus strains, except for one clinical strain of A. fumigatus whose MIC was 4.0mg/L. Posaconazole (≤0.25mg/L) also showed appreciable activity against both species of Aspergillus, except for six A. fumigatus strains with relatively higher MICs (0.5mg/L). The MICs for Amphotericin B ranged from 0.06 to 1.0mg/L for A. fumigatus, but were much higher (0.5-8.0mg/L) for A. flavus. Among the echinocandins, caspofungin showed a geometric mean of 0.078 and 0.113 against the clinical and environmental strains of A. flavus, respectively, but had elevated minimal effective concentrations (MECs) for seven of the A. fumigatus strains. Anidulafungin and micafungin exhibited considerable activity against both A. fumigatus and A. flavus isolates, except for one environmental isolate of A. fumigatus that showed an MEC of 1mg/L to micafungin. Our study proposes that a detailed investigation of the antifungal susceptibility of the genus Aspergillus from different regions of Brazil is necessary for establishing a response profile against the different classes of antifungal agents used in the treatment of aspergillosis.
Acta Scientiae Veterinariae | 2014
Deise Flores Santurio; Francielli Pantella Kunz de Jesus; Régis Adriel Zanette; Karine Bizzi Schlemmer; Andressa Fraton; Leadir Lucy Martins Fries
Ciencia Rural | 2015
Carla Weiblen; Gustavo Machado; Francielli Pantella Kunz de Jesus; Janio Morais Santurio; Régis Adriel Zanette; Daniela Isabel Brayer Pereira; Gustavo Nogueira Diehl; Lucila Carboneiro dos Santos; Luis Gustavo Corbellini; Sônia de Avila Botton
Medical Mycology | 2016
Tatiana Corrêa Ribeiro; Carla Weiblen; Sônia de Avila Botton; Daniela Isabel Brayer Pereira; Francielli Pantella Kunz de Jesus; Camila Marina Verdi; Letícia Trevisan Gressler; Luís Antônio Sangioni; Janio Morais Santurio
Veterinária e Zootecnia | 2011
C. Lautert; Francielli Pantella Kunz de Jesus; Régis Adriel Zanette; Janio Morais Santurio; Sydney Hartz Alves