Francis Cogé
Collège de France
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Featured researches published by Francis Cogé.
Journal of Biological Chemistry | 2008
Adeline Giganti; Marianne Rodriguez; Benjamin Fould; Natacha Moulharat; Francis Cogé; Pascale Chomarat; Jean-Pierre Galizzi; Philippe Valet; Jean-Sébastien Saulnier-Blache; Jean A. Boutin; Gilles Ferry
Autotaxin is a type II ectonucleotide pyrophosphate phosphodiesterase enzyme. It has been recently discovered that it also has a lysophospholipase D activity. This enzyme probably provides most of the extracellular lysophosphatidic acid from lysophosphatidylcholine. The cloning and tissue distribution of the three isoforms (imaginatively called α, β, and γ) from human and mouse are reported in this study, as well as their tissue distribution by PCR in the human and mouse. The fate of the α isoform from human was also studied after purification and using mass spectrometry. Indeed, this particular isoform expresses the intron 12 in which a cleavage site is present, leading to a rapid catabolism of the isoform. For the human isoform γ and the total autotaxin mRNA expression, quantitative PCR is presented in 21 tissues. The isoforms were expressed in two different hosts, insect cells and Chinese hamster ovary cells, and were highly purified. The characteristics of the six purified isoforms (pH and temperature dependence, Km and Vmax values, and their dependence on metal ions) are presented in this study. Their sensitivity to a small molecule inhibitor, hypericin, is also shown. Finally, the specificity of the isoforms toward a large family of lysophosphatidylcholines is reported. This study is the first complete description of the reported autotaxin isoforms.Autotaxin is a type II ectonucleotide pyrophosphate phosphodiesterase enzyme. It has been recently discovered that it also has a lysophospholipase D activity. This enzyme probably provides most of the extracellular lysophosphatidic acid from lysophosphatidylcholine. The cloning and tissue distribution of the three isoforms (imaginatively called alpha, beta, and gamma) from human and mouse are reported in this study, as well as their tissue distribution by PCR in the human and mouse. The fate of the alpha isoform from human was also studied after purification and using mass spectrometry. Indeed, this particular isoform expresses the intron 12 in which a cleavage site is present, leading to a rapid catabolism of the isoform. For the human isoform gamma and the total autotaxin mRNA expression, quantitative PCR is presented in 21 tissues. The isoforms were expressed in two different hosts, insect cells and Chinese hamster ovary cells, and were highly purified. The characteristics of the six purified isoforms (pH and temperature dependence, K(m) and V(max) values, and their dependence on metal ions) are presented in this study. Their sensitivity to a small molecule inhibitor, hypericin, is also shown. Finally, the specificity of the isoforms toward a large family of lysophosphatidylcholines is reported. This study is the first complete description of the reported autotaxin isoforms.
FEBS Letters | 2007
Gilles Ferry; Adeline Giganti; Francis Cogé; Fabien Bertaux; Kader Thiam; Jean A. Boutin
Autotaxin is a member of the phosphodiesterase family of enzymes, (NPP2). It is an important secreted protein found in conditioned medium from adipocytes. It also has a putative role in the metastatic process. Based on these observation, further validation of this potential target was necessary, apart from the classical biochemical ones. The construction of a knock out mouse strain for ATX was started. In this paper, we report the generation of a mouse line displaying an inactivated ATX gene product. The KO line was designed in order to generate a functional inactivation of the protein. In this respect, the threonine residue T210 was replaced by an alanine (T210A) leading to a catalytically inactive enzyme. If the experimental work was straight forward, we disappointedly discovered at the final stage that the breeding of heterozygous animals, ATX −/+, led to the generation of a Mendelian repartition of wild‐type and heterozygous, but no homozygous were found, strongly suggesting that the ATX deletion is lethal at an early stage of the development. This was confirmed by statistical analysis. Although other reported the same lethality for attempted ATX−/− mice generation [van Meeteren, L.A., Ruurs, P., Stortelers, C., Bouwman, P., van Rooijen, M.A., Pradère, J.P., Pettit, T.R., Wakelam, M.J.O., Saulnier‐Blache, J.S., Mummery, C.L., Moolenar, W.H. and Jonkers, J. (2006) Autotaxin, a secreted lysophospholipase D, is essential for blood vessel formation during development, Mol. Cell. Biol. 26, 5015–5022; Tanaka, M., Okudaira, S., Kishi, Y., Ohkawa, R., Isei, S., Ota, M., Noji, S., Yatomi, Y., Aoki, J., and Arai, H. (2006) Autotaxin stabilizes blood vessels and is required for embryonic vasculature by producing lysophosphatidic acid, J. Biol. Chem. 281, 25822–25830], they used more drastic multiple exon deletions in the ATX gene, while we chose a single point mutation. To our knowledge, the present work is the first showing such a lethality in any gene after a point mutation in an enzyme catalytic site.
British Journal of Pharmacology | 2009
Francis Cogé; Sp Guenin; I Fery; M Migaud; Séverine Devavry; C Slugocki; Céline Legros; Christine Ouvry; W Cohen; N Renault; Olivier Nosjean; B Malpaux; Philippe Delagrange; Jean A. Boutin
Background and purpose: For many years, it was suspected that sheep expressed only one melatonin receptor (closely resembling MT1 from other mammal species). Here we report the cloning of another melatonin receptor, MT2, from sheep.
Chemico-Biological Interactions | 2010
Gilles Ferry; Sabrina Hecht; Sylvie Berger; Natacha Moulharat; Francis Cogé; Gérald Guillaumet; Veronique Leclerc; Said Yous; Philippe Delagrange; Jean A. Boutin
Quinone reductase 2 is a cytosolic enzyme which catalyses the reduction of quinones, such as menadione and coenzymes Q. Despite a relatively close sequence-based resemblance to NAD(P)H:quinone oxidoreductase 1 (QR1), it has many different features. QR2 is the third melatonin binding site (MT3). It is inhibited in the micromolar range by melatonin, and does not accept conventional phosphorylated nicotinamides as hydride donors. QR2 has a powerful capacity to activate quinones leading to unexpected toxicity situations. In the present paper, we report the characterization of three QR2 modulators: melatonin, resveratrol and S29434. The latter compound inhibits QR2 activity with an IC(50) in the low nanomolar range. The potency of the modulators ranged as follows, from the least to the most potent: melatonin<resveratrol<S29434. These molecular tools might permit to explore and better understand the relationship existing between QR2 catalytic activity and the various pathological situations in which QR2 has a key role.
Journal of Biomolecular Screening | 2010
Jean-Pierre Cabaniols; Christine Ouvry; Véronique Lamamy; Isabelle Fery; Marie-Laure Craplet; Natacha Moulharat; Sophie-Pénélope Guénin; Stéphane Bedut; Olivier Nosjean; Gilles Ferry; Séverine Devavry; Cécile Jacqmarcq; Céline Lebuhotel; Luc Mathis; Christophe Delenda; Jean A. Boutin; Philippe Duchâteau; Francis Cogé
The development of cell-based assays for high-throughput screening (HTS) approaches often requires the generation of stable transformant cell lines. However, these cell lines are essentially created by random integration of a gene of interest (GOI) with no control over the level and stability of gene expression. The authors developed a targeted integration system in Chinese hamster ovary (CHO) cells, called the cellular genome positioning system (cGPS), based on the stimulation of homologous gene targeting by meganucleases. Five different GOIs were knocked in at the same locus in cGPS CHO-K1 cells. Further characterization revealed that the cGPS CHO-K1 system is more rapid (2-week protocol), efficient (all selected clones expressed the GOI), reproducible (GOI expression level variation of 12%), and stable over time (no change in GOI expression after 23 weeks of culture) than classical random integration. Moreover, in all cGPS CHO-K1 targeted clones, the recombinant protein was biologically active and its properties similar to the endogenous protein. This fast and robust method opens the door for creating large collections of cell lines of better quality and expressing therapeutically relevant GOIs at physiological levels, thereby enhancing the potential scope of HTS.
American Journal of Physiology-heart and Circulatory Physiology | 2016
Stéphane Bedut; Christine Seminatore-Nole; Véronique Lamamy; Sarah Caignard; Jean A. Boutin; Olivier Nosjean; Jean-Philippe Stephan; Francis Cogé
Cardiomyocytes derived from human embryonic stem cells (hESCs) or induced pluripotent stem cells (hiPSCs) are increasingly used for in vitro assays and represent an interesting opportunity to increase the data throughput for drug development. In this work, we describe a 96-well recording of synchronous electrical activities from spontaneously beating hiPSC-derived cardiomyocyte monolayers. The signal was obtained with a fast-imaging plate reader using a submillisecond-responding membrane potential recording assay, FluoVolt, based on a newly derived voltage-sensitive fluorescent dye. In our conditions, the toxicity of the dye was moderate and compatible with episodic recordings for >3 h. We show that the waveforms recorded from a whole well or from a single cell-sized zone are equivalent and make available critical functional parameters that are usually accessible only with gold standard techniques like intracellular microelectrode recording. This approach allows accurate identification of the electrophysiological effects of reference drugs on the different phases of the cardiac action potential as follows: fast depolarization (lidocaine), early repolarization (nifedipine, Bay K8644, and veratridine), late repolarization (dofetilide), and diastolic slow depolarization (ivabradine). Furthermore, the data generated with the FluoVolt dye can be pertinently complemented with a calcium-sensitive dye for deeper characterization of the pharmacological responses. In a semiautomated plate reader, the two probes used simultaneously in 96-well plates provide an easy and powerful multiparametric assay to rapidly and precisely evaluate the cardiotropic profile of compounds for drug discovery or cardiac safety.
British Journal of Pharmacology | 2003
Christelle Mérial-Kieny; Michel Lonchampt; Francis Cogé; Patrick Verwaerde; Jean-Pierre Galizzi; Jean A. Boutin; Max Lafontan; Nigel Levens; Jean Galitzky; Michel Félétou
Endothelin‐1 (ET‐1) and tumor necrosis factor α (TNFα) by their action on adipocytes have been independently linked to the pathogenesis of insulino‐resistance. In isolated adipocytes, TNFα induces the expression of the inducible nitric oxide synthase (iNOS). The purpose of the present work was, in the 3T3‐F442A adipocyte cell line, to characterise TNFα‐induced iNOS expression and to determine whether or not ET‐1 could influence TNFα‐induced iNOS expression and NO production. In differentiated 3T3‐F442A, treatment with TNFα (20 ng ml−1) induced the expression of a functional iNOS as demonstrated by nitrite assay, Western blot, reverse transcription–polymerase chain reaction and Northern blot analysis. TNFα‐induced iNOS expression requires nuclear factor κB activation, but does not necessitate the activation of the PI‐3 kinase/Akt and P38–MAP kinase pathways. ET‐1, but not ET‐3, inhibited the TNFα‐induced expression of iNOS protein and mRNA as well as nitrite production. The effects of ET‐1 were blocked by a specific ETA (BQ123, pA2 7.4) but not by a specific ETB receptor antagonist (BQ788). 3T3‐F442A adipocytes express the mRNAs for prepro‐ET‐1 and the ET‐A receptor subtype, but not for the ET‐B subtype. The inhibitory effect of ET‐1 was not affected by bisindolylmaleimide, SB 203580 or indomethacin, inhibitors of protein kinase C, p38‐MAP kinase and cyclooxygenase, respectively, and was not associated with cAMP production. However, the effect of ET‐1 was partially reversed by wortmannin, suggesting the involvement of PI3 kinase in the transduction signal of ET‐1. Differentiated 3T3‐F442A adipocytes did not release ET‐1 with or without exposure to TNFα, although the mRNA for preproET‐1 was detected in both pre‐ and differentiated adipocytes. Thus, these results confirm that adipocytes are a target for circulating ET‐1 and demonstrate that the activation of the ETA receptor subtype can prevent TNFα‐induced iNOS expression.
European Journal of Pharmacology | 2012
Séverine Devavry; Céline Legros; Chantal Brasseur; William Cohen; Sophie-Pénélope Guénin; Philippe Delagrange; Benoît Malpaux; Christine Ouvry; Francis Cogé; Olivier Nosjean; Jean A. Boutin
The main melatonin receptors are two G-protein coupled receptors named MT(1) and MT(2). Having described the molecular pharmacology of the human versions of these receptors, we turned to two of the three species most useful in studying melatonin physiology: rat and sheep (a diurnal species used to understand the relationship between circadian rhythm and depression). We also employed previously used compounds to describe the mouse melatonin receptors; despite the early cloning of mouse receptors, few molecular pharmacology studies on these receptors exist. To our surprise, we detected no major differences between the data obtained from mice and those from other species.
Biochemical and Biophysical Research Communications | 1990
Francis Cogé; Monique Krieger-Pouliet; François Gros; Jean Thibault
The L-DOPA decarboxylase mRNA levels were determined by a sensitive S1 nuclease method in four organs and one tumor of adult rat. S1 mapping analysis, with probes corresponding to the mRNA coding region, showed that this region is conserved in all L-DOPA decarboxylase mRNA of neuronal and non-neuronal tissues. The mRNA was not very abundant; its representation varies approximately from 0.00035% of total RNA in the mid brain to 0.013% of total mRNA in the pheochromocytoma. A strong correlation between mRNA level and enzyme amount was observed (correlation coefficient = 0.99). The results indicate that the level of mRNA is a primary factor determining the L-DOPA decarboxylase level.
Archives of Biochemistry and Biophysics | 2008
Jean A. Boutin; Carine Saunier; Sophie-Pénélope Guénin; Sylvie Berger; Natacha Moulharat; Arnaud Gohier; Philippe Delagrange; Francis Cogé; Gilles Ferry
Melatonin is a neurohormone implicated in both biorhythm synchronization and neuroprotection from oxidative stress. Its functions are mediated by two G-protein-coupled-receptors (MT1 and MT2) and MT3, which corresponds to quinone oxidoreductase 2 (QR2). To determine the binding site of QR2 for melatonin, point mutations of residues crucial for the enzymatic activity of hQR2 were performed. The substitution of the hydrophobic residues Phe126, Ile128 and Phe178 by tyrosines at the active site significantly increased enzymatic activity and decreased the affinity of a structural analog of melatonin, the 2[(125)I]iodo-MCANAT. The mutation of residues implicated in zinc chelating (His(173); His(177)) had no effect on radioligand binding. Destabilisation of the cofactor FAD by mutation N18E showed that 2[(125)I]iodo-MCANAT binding was closely linked to the conformational integrity of human QR2. Surprisingly, the mutations C222F and N161A, which are distant from the determined binding site of the ligand, increased the affinity of 2[(125)I]iodo-MCANAT for hQR2. What seems to better explain the binding variations among the mutants are the activity recorded with BNAH and coenzyme Q1. Various hypotheses are discussed based on the various parameters used in the study: nature of the substrates and co-substrates and nature of the amino acid changes. This study, which constitutes the first structural analysis of hQR2, should enable to better understand the biological role of melatonin on this enzyme and particularly, the discrepancies between the pharmacologies of the melatonin binding site (MT3) and the QR2 catalytic activity.