Francis E. Ray

Routes of metabolism of [36Cl] ring-substituted monochloroacetanilides☆☆☆

Abstract The excreted monohydroxy and deacetylated metabolites of 2-, 3- and 4-[ 36 Cl]chloroacetanilide in the urine of rats were determined. The extent of ortho-hydroxylation (relative to the amino group) decreased in the same order as the relative toxicities and antipyretic activities of the administered drugs: 4- 3- 2-. Neither total hydroxylation nor para -hydroxylation followed this order. Deacetylation of the parent compound increased as the toxicity and antipyretic activity decreased. Conjugation of the hydroxylated metabolites as etheral sulfates and glucuronides was observed.

Effect of tumors on liver and spleen uptake of radioactive material.

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Effect of Ionizing Radiation on 9,10-Dimethyl-1,2-Benzanthracene Tumorigenesis

9,10-Dimethyl-1,2-benzanthracene (DMBA) was applied to the skin of female Swiss albino mice in the following amounts; 0.00002, 0.12, 0.20, 0.40, 0,80, and 1.60 mg. The lowest dose produced no skin papillomas; 0,12 mg resulted in one tumor appearing at 34 weeks; there was an increase in the tumor incidence from this dosage up to the 0.80 mg dose level. Exposure of the mouse skin to 200, 400, or 600 r of GAMMA irradiation from a cobalt-60 source. or 800, 1600, or 3200 r of x irradiation failed to produce skin tumors. No enhancement of skin tumorigenic action was found when 200 r of gamma irradiation was applied concurrently with 0.12 mg of DMBA, or when 200, 400, or 600 r of gamma irradiation was applied concurrently with 0,00002 mg of DMBA. X irradiation with 1600 and 3200 r was found to inhibit the tumorigenic activity of 0.20 mg of DMBA when applied either after or prior to the chemical, With a dosage level of 0.80 mg of DMBA, however, these levels of x irradiation exhibited no inhibition.

The metabolism of 2-aminofluorene in the guinea pig.

Abstract The distribution of 2-aminofluorene following a single intraperitoneal injection has been studied in the guinea pig using the diazotization method, and the results have been compared with those obtained from similar studies in the rat. Less free diazotizable material was found in the guinea pig than in the rat, but more in combined form was present in the guinea pig. The total amount of free plus combined 2-aminofluorene was also greater in the guinea pig. These data show important differences in the metabolism of the two species and may account for the reportedly greater resistance of the guinea pig to chemical carcinogenesis.

Effect of rapid tissue growth on the uptake of fluorene-2, 7-di-(sulfonamido-2-naphthalene)-S35 by the liver and spleen of rats and hamsters.

Effect of Rapid Tissue Growth on the Uptake of Fluorene-2,7-Di-(Sulfonamido-2-Naphthalene)-S 35 by the Liver and Spleen of Rats and Hamsters

Gastric ascorbic acid in the gastritic rat.

Summary Concentrations of reduced, oxidized, and total ascorbic acid in the fore-stomach, glandular stomach, and adrenals of the rat have been reported. Direct contact of a eugenol emulsion with the gastric mucosa resulted in a gastritis and a 13% decrease in the total gastric ascorbic acid. Systemic absorption of the eugenol, avoiding direct gastric contact, did not induce these changes. A decrease in adrenal ascorbic acid after eugenol administration suggests a systemic stress effect also associated with the presence of the eugenol, but distinct from the direct gastric effect.

Effect of radiation on distribution of S35-labeled sulfonamide.

Summary 1. Male and female Wistar rats and CAF1/Jax mice show a reduction in weight of thymus and spleen 48 hours following whole-body irradiation of 150 r. 2. Unlike presence of a tumor in animal body, this irradiation dose does not affect uptake of fluorene-2,7-di-(sulfonamido-2-naphthalene) - S35 by liver and spleen. 3. Significantly different localization of the S35-labeled compound is found in blood cells and thymus of irradiated as compared to nonirradiated male mice.