Francisco J. Pérez-Vázquez

Exposure to indoor air pollutants (polycyclic aromatic hydrocarbons, toluene, benzene) in Mexican indigenous women.

UNLABELLED Indoor air pollution is considered to be a serious public health issue in Mexico; therefore, more studies regarding this topic are necessary. In this context, we assessed exposure to polycyclic aromatic hydrocarbons (PAHs) and volatile organic compounds in: (i) women who use firewood combustion (indoor) for cooking and heating using traditional open fire; (ii) women who use firewood combustion (outdoor) for cooking and heating using traditional open fire; and (iii) women who use LP gas as the principal energy source. We studied 96 healthy women in San Luis Potosi, México. Urine samples were collected, and analyses of the following urinary exposure biomarkers were performed by high-performance liquid chromatography: 1-hydroxypyrene (1-OHP), trans, trans-muconic acid, and hippuric acid (HA). The highest levels of 1-OHP, trans, trans-muconic acid, and HA were found in communities where women were exposed to indoor biomass combustion smoke (or products; geometric mean ± s.d., 3.98 ± 5.10 μmol/mol creatinine; 4.81 ± 9.60 μg/l 1-OHP; 0.87 ± 1.78 mg/g creatinine for trans, trans-muconic acid; and 1.14 ± 0.91 g/g creatinine for HA). Our findings indicate higher exposure levels to all urinary exposure biomarkers studied in women who use indoor firewood combustion for cooking and heating (using traditional open fire). PRACTICAL IMPLICATIONS High mean levels of 1-hydroxypyrene, t,t-muconic acid, and hippuric acid were found in women who use firewood combustion (indoor) for cooking and heating using traditional open fire and taking into account that millions of women and children in Mexico are living in scenarios similar to those studied in this report, the assessment of health effects in women and children exposed to polycyclic aromatic hydrocarbons and volatile organic compounds is urgently needed. Moreover, it is immediately necessary an intervention program to reduce exposure.

DNA damage in Mexican children living in high-risk contaminated scenarios

The aim of this study was to evaluate the deoxyribonucleic acid (DNA) damage (as a biomarker of biological effects) in children living in areas at high risk of contamination in Mexico using the comet assay. The alkaline comet assay was performed in order to assess DNA damage levels in blood cells of 276 children living in eleven communities in four states of Mexico. Moreover, levels of arsenic and 1-hydroxypyrene (1-OHP) in urine and lead and total DDT [sum of 1,1-dichloro-2,2-bis(p-chlorophenyl) ethylene (DDE) and 1,1-bis(p-chlorophenyl)-2,2,2-trichloroethane (DDT)] in blood were quantified. We found urinary 1-OHP levels between <LOD and 14.5 μmol/mol creatinine; for arsenic, the urinary levels were 3.5-180 μg/g creatinine (range). Lead levels in blood ranged from 0.5 to 24 μg/dL and finally, the levels of total DDT (DDE and DDT) ranged from <LOD to 32,000 ng/g lipid. Regarding DNA damage (comet assay), the most important finding in our study was that children exposed to a chemical mixture [high levels of exposure to polycyclic aromatic hydrocarbons (PAHs) and DDT were found] had the significant highest DNA damage level (p<0.05) in their blood cells (olive tail moment=7.5±3.5), when compared with DNA damage levels in children living in the other scenarios assessed in this work. Finally, significant correlations were observed between urinary arsenic levels (r=0.32, p<0.05); urinary 1-OHP levels (r=0.65, p<0.01); total DDT in blood levels (r=0.59, p<0.01) and DNA damage. In conclusion, the data indicates that children living in areas which are at high risk of contamination showed high levels of biomarkers of exposure in urine or blood. Moreover, the exposure levels contribute to DNA damage and suggest an increased health risk in studied sites at risk of great pollution.

Concentrations of persistent organic pollutants (POPs) in human blood samples from Mexico City, Mexico.

Studies in Mexico have demonstrated exposure to persistent organic pollutants (POPs) in people living in different sites through the country. However, studies evaluating exposure to POPs in people living in Mexico City (one of most contaminated places in the world) are scarce. Therefore, the aim of this study was to assess the levels of polybrominated diphenyl ethers (PBDEs), polychlorinated biphenyls (PCBs), dichlorodiphenyltrichloroethane (DDT) and its metabolite dichlorodiphenyldichloroethylene (DDE) in the blood as exposure biomarkers in people living in Mexico City. A total of 123 participants (blood donors aged 20-60 years) were recruited during 2010 in Mexico City. Quantitative analyses of blood samples were performed using gas chromatography coupled with mass spectrometry. Levels of the assessed compounds ranged from non-detectable (<LOD) to 350 ng/g lipid; from 8.20 to 91.0 ng/g lipid and from <LOD to 34.0 ng/g lipid for total PBDEs, total PCBs and total DDT, respectively. The current study indicates POP exposure in the people assessed and highlights the need for further biomonitoring studies of these POPs in the region. In this regard, biomonitoring of toxins on a global scale may be the first step towards the prevention of toxin-induced illnesses in the population.

Using urinary 1-hydroxypyrene concentrations to evaluate polycyclic aromatic hydrocarbon exposure in women using biomass combustion as main energy source

Abstract Context: The use of solid fuels for cooking and heating is likely to be the largest source of indoor air pollution on a global scale. Objective: The aim of this study was to investigate the urinary excretion of 1-hydroxypyrene (1-OHP) in women living in a rural community, where biomass combustion is used as main energy source during a working day. Materials and methods: The study was performed on urine samples collected in 2012, of 30 healthy women who were residents of a rural community in San Luis Potosi, Mexico. Urine was collected from each woman at three time points (morning, post-lunch and evening) during a working day. The analysis of urinary 1-OHP was performed using high-performance liquid chromatography (HPLC). Also, a health-risk assessment was conducted. Results: The highest levels of 1-OHP in this study were found in the samples taken in the evening (geometric mean ± SD; 0.36 ± 0.13 µg/g creatinine). However, no significant differences among 1-OHP concentrations in the evening and post-lunch samples (0.27 ± 0.10 µg/g creatinine; 0.58 ± 0.67 µg/L) were observed. But, the 1-OHP levels (0.17 ± 0.13 µg/g creatinine; 0.19 ± 0.21 µg/L) found in samples collected in the morning were significantly lower than the 1-OHP levels found in samples collected during post-lunch and evening time. Discussion and conclusion: The data shown in this study demonstrated an increment in the exposure levels to PAHs in women across the shift. However, no health risk was found in this study.

Urinary 1-hydroxypyrene concentration from Mexican children living in the southeastern region in Mexico.

Biomass combustion indoors has been associated with generation of various pollutants, such as carbon monoxide, formaldehyde, respirable particles, toluene, benzene and polycyclic aromatic hydrocarbons (PAHs), among others. In this context, the aim of this study was to evaluate the urinary 1-hydroxypyrene (as a biomarker exposure to PAHs) levels in children living in three states in Mexico. We evaluated children living in communities that use biomass fuels to cook and to heat homes in the next states: Chiapas, Oaxaca and Quintana Roo. We found similar levels of 1-OHP in urine of children living in the three studied states, with mean levels of 3.5 ± 1.0; 4.5 ± 2.8 and 4.4 ± 2.5 μg/L (geometric mean ± standard deviation), respectively for Chiapas, Oaxaca and Quintana Roo. In conclusion, our data indicate high exposure levels to PAHs in children living in the states studied in this work.

Analysis of cytotoxic effects of silver nanoclusters on human peripheral blood mononuclear cells 'in vitro'.

The antimicrobial properties of silver nanoparticles (AgNPs) have made these particles one of the most used nanomaterials in consumer products. Therefore, an understanding of the interactions (unwanted toxicity) between nanoparticles and human cells is of significant interest. The aim of this study was to assess the in vitro cytotoxicity effects of silver nanoclusters (AgNC, < 2 nm diameter) on peripheral blood mononuclear cells (PBMC). Using flow cytometry and comet assay methods, we demonstrate that exposure of PBMC to AgNC induced intracellular reactive oxygen species (ROS) generation, DNA damage and apoptosis at 3, 6 and 12 h, with a dose‐dependent response (0.1, 1, 3, 5 and 30 µg ml–1). Advanced electron microscopy imaging of complete and ultrathin‐sections of PBMC confirmed the cytotoxic effects and cell damage caused by AgNC. The present study showed that AgNC produced without coating agents induced significant cytotoxic effects on PBMC owing to their high aspect ratio and active surface area, even at much lower concentrations (<1 µg ml–1) than those applied in previous studies, resembling what would occur under real exposure conditions to nanosilver‐functionalized consumer products. Copyright

Variability in DDT-induced apoptosis in Mexican indigenous populations

Context: In previous studies, we showed that DDT and its metabolites are able to induce apoptosis of peripheral blood mononuclear cells (PBMC) “in vitro” and “in vivo”, by a mechanism involving oxidative stress. Objective: The objective of this study was to evaluate the mechanism by which DDT induces apoptosis in PBMC in children exposed to DDT and its metabolites. Materials and methods: Eligibility criteria included children who: (1) have lived in the selected community since birth, (2) were between 6 and 14 years of age at the time of the study, (3) had not been exposed to medicaments or tobacco smoke, and (4) had had no infectious diseases in the month prior to the study. DDT and its metabolites were quantified using gas chromatography with an electron capture detector, PBMC apoptosis was measured using the TUNEL assay, DNA damage and oxidative damage were studied using the comet assay. Results: Apoptosis correlated to DDE exposure (p = 0.040), as previously found. DNA damage also correlated to DDT (p = 0.005) and DDE (p = 0.004) levels. However, neither exposure to DDT or DDE and oxidative damage, nor oxidative damage and apoptosis, were significantly correlated. Children living in Lacanja, Chiapas, one of the communities studied in this work, had the highest levels of exposure to DDT and its metabolites, yet had the lowest percentage of apoptosis. Conclusion: Resistance to DDE-induced apoptosis was found in children from one community. Further studies are needed in order to understand the mechanism involved in this apoptosis resistance.

Plasma asymmetric dimethylarginine (ADMA) levels in Mexican women exposed to polycyclic aromatic hydrocarbons (PAHs): A preliminary study

Recent studies indicate that exposure to environmental pollutants (as polycyclic aromatic hydrocarbons) is a very important risk factor for development of cardiovascular diseases (CVDs). Correspondingly, in recent times asymmetric dimethylarginine (ADMA) has been proposed as a new and meaningful biomarker predictor for the risk of CVDs. Therefore, the objective of this study was to evaluate plasma ADMA concentrations in Mexican women (n=155) exposed to polycyclic aromatic hydrocarbons (PAHs). Urinary 1-hydroxypyrene [(1-OHP), exposure biomarker for PAHs] levels were quantified using a high performance liquid chromatography (HPLC) technique and plasma ADMA concentrations were analyzed using a commercially available ELISA kit. Urinary 1-OHP levels in all women assessed ranged from <LOD to 8.80μmol/mol creatinine. Regarding plasma ADMA concentrations, the mean levels ranged from 0.66 to 1.00μmol/L. Moreover, a significantly relationship was found between plasma ADMA concentrations and urinary 1-OHP levels (p=0.005; r=0.390). In conclusion, the results showed in this study demonstrated that the urinary 1-OHP levels were significant predictors of plasma ADMA concentrations in Mexican women exposed to PAHs. Although with due caution regarding the low representativeness of the present screening, it can be considered as a preliminary work to perform prospective studies including greater number of individuals and focusing in a more complete characterization of the effects produced by PAHs exposure on cardiovascular health.

Human health risks associated with heavy metals in soil in different areas of San Luis Potosí, México

ABSTRACT The aim of this study was to develop a health risk assessment in different areas of San Luis Potosí, México. Four heavy metals (arsenic, mercury, cadmium, and lead) were analyzed in soil from communities assessed. The mean arsenic concentration was significantly higher (p < .05) in the city of San Luis Potosí (51.85 mg/kg) compared to the other assessed areas (5.52–8.43 mg/kg). For cadmium, the mean concentration was significantly higher (p < .05) in Santa Maria Picula (7.46 mg/kg) than in the other areas (3.72–4.15 mg/kg). Regarding mercury levels, a significantly higher (p < .05) mean concentration was found in Mezquitic (1.54 mg/kg) compared to other areas (0.56–0.81 mg/kg). Lastly, when comparing the mean lead concentration in the city of San Luis Potosí (108 mg/kg), it was found to be significantly lower (p < .05) than in other areas (219–227 mg/kg). Subsequently, a probabilistic health risk assessment was performed, ingestion was the major exposure pathway for all four metals. Maximum cumulative hazard index (HI) values showed higher risk in all sampled locations (HIs > 1.0), suggesting that these sites can pose a non-carcinogenic risk to the populations (children) living in those areas. This study highlights the necessity of establishing a biomonitoring program for the surveillance of the child populations living in the assessed locations.

DDT and DDE concentrations in the blood of Mexican children residing in the southeastern region of Mexico.

The aim of this study was to assess the levels of dichlorodiphenyltrichloroethane (DDT) and dichlorodiphenyldichloroethylene (DDE) in the blood of children living in the southeastern region of Mexico. In this study, we found high levels of DDT and its principal metabolite (DDE) in the blood of children residing in the communities studied. The levels of total DDT found in our study ranged from 4,676.4 ng/g lipid to 64,245.2 ng/g lipid. All of the children in the study had detectable levels of DDT and/or DDE. In conclusion, our data indicate that children living within the study areas are exposed to high levels of DDT and DDE. Moreover, these results can be used as a trigger to revisit local policies on environmental exposures.